Changes in total RNA, polyadenylated RNA, and actin mRNA during meiotic maturation of mouse oocytes

Bachvarova, Rosemary F.; Leon, Victor De; Johnson, Andrew C.; Kaplan, G; Paynton, Barbara V.

doi:10.1016/0012-1606(85)90036-3

Cited by 189 publications

(123 citation statements)

References 30 publications

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“…This process, called cytoplasmic polyadenylation, was initially thought to be confined to gametes and embryos, although recent evidence suggests that it is also important in neurons (23). The molecular mechanisms regulating cytoplasmic polyadenylation have been studied primarily in mouse (24,25) and in Xenopus oocytes (26)(27)(28)(29) and appear to be highly conserved. In addition to the nuclear cleavage and polyadenylation signal, AAUAAA, a second cisacting sequence in the 3Ј UTR of the mRNA, the cytoplasmic polyadenylation element (CPE) (consensus sequence UUUUUA 1-2U), is necessary for cytoplasmic polyadenylation (30).…”

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confidence: 99%

An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos

Seli

Lalioti

Flaherty

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

118

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Gene expression during oocyte maturation, fertilization, and early embryo development until zygotic gene activation is regulated mainly by translational activation of maternally derived mRNAs. This process requires the presence of a poly(A)-binding protein. However, the cytoplasmic somatic cell poly(A)-binding protein (PABP1) is not expressed until later in embryogenesis. We recently identified an embryonic poly(A)-binding protein (ePAB) in Xenopus. ePAB is the predominant cytoplasmic PABP in Xenopus oocytes and early embryos and prevents deadenylation of mRNAs, suggesting its importance in the regulation of gene expression during early Xenopus development. Here we report the identification of the mouse ortholog of Xenopus ePAB. The mouse ePAB gene on chromosome 2 contains 14 exons that specify an alternatively spliced mRNA encoding a protein of 608 or 561 aa with Ϸ65% identity to Xenopus ePAB. Mouse ePAB mRNA is expressed in ovaries and testis but not in somatic tissues. In situ hybridization localizes ePAB RNA to oocytes and confirms its absence from surrounding somatic cells in the mouse ovary. During early development, mouse ePAB is expressed in prophase I and metaphase II oocytes and one-cell and two-cell embryos and then becomes undetectable in four-or-more-cell embryos. In contrast, PABP1 mRNA expression is minimal in oocytes and early embryos until the eight-cell stage when it increases, becoming predominant at the blastocyst stage. The expression of mouse ePAB before zygotic gene activation argues for its importance in translational activation of maternally derived mRNAs during mammalian oocyte and early preimplantation embryo development.translational activation ͉ embryogenesis ͉ oogenesis M echanisms for establishing the germline and carrying out oogenesis in evolutionarily distant animals exhibit common themes. Gametes develop from primordial germ cells that are set aside during early embryogenesis (1). In most metazoans, primordial germ cells have an extragonadal origin and migrate to reach the somatic gonad, where they proliferate by mitosis to form oocytes (1). Oocytes, in turn, enter meiosis only to be arrested at the prophase of the first meiotic division (2, 3). This first meiotic arrest may last up to a few years in Xenopus and several decades in humans and is characterized by synthesis and storage of large quantities of dormant mRNA (4, 5). The resumption of meiosis is stimulated by progesterone in Xenopus (6, 7) or by gonadotropins in mouse and human (8, 9) and marks the onset of oocyte maturation.Oocyte maturation is accompanied by a complex network of translational activation and repression of dormant maternal mRNAs (10-13), whereas transcription is limited at best. These maternal mRNAs drive the oocyte's reentry into meiosis and control the rate of mitosis during the early cleavage divisions of the embryo (13-16). The transcriptional silencing that begins with oocyte maturation persists during the initial mitotic divisions of the embryonic cells. In Xenopus, after 12 rapid synchronous cle...

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mentioning

confidence: 99%

An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos

Seli

Lalioti

Flaherty

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

118

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“…As more and more pro-hormones, hormones (and their mRNAs, Einspanier et al, 1986) are discovered in the ovary some may be candidates as recently mentioned for the SRIF (pig, Mori et al, 1985) and for the anti-mullerian hormone (AMH) (rat, Takahashi et al, 1986 Lintern-Moore, 1978 ;Kaplan et al, 1982 ;Bachvarova et al, 1985). After the growth period a low rate of RNA synthesis is maintained, even if nucleolus compaction indicates that rRNA transcription is impaired.…”

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Mammalian oocyte maturation

Thibault¹,

Szöllősi²,

Gérard³

1987

Reprod. Nutr. Dévelop.

207

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“…En ovocitos bovinos y porcinos, la unión de eIF4E al complejo eIF4F se relaciona con un aumento en la fosforilación de eIF4E y es un indicativo de que el ovocito está en metafase II (35,36) . Además, este proceso promueve la unión del factor específico de la poliadenilación citoplásmica (CPSF) con la secuencia 5´AAUAAA´3; que a su vez permite que la proteína embrionaria de unión de adeninas (ePAB) adicione adeninas al UTR´3, lo que provoca que el UTR´5 se estabilice para la correcta traducción del RNAm (37)(38)(39)(40)(41) . En ratón la expresión de ePAB se presenta en la profase I y metafase II de ovocitos y en embriones de 2 a 4 células, ya que se activa el genoma embrionario; esta expresión es suprimida, y debido a esto es considerada un RNAm materno (42) .…”

Section: Inactivación/activación De Los Rnam Maternosunclassified

“…In cattle and swine oocytes, the union of eIF4E to the complex eIF4F is related to an increase in eIF4E phosphorylation and is an indicative that the oocyte is at metaphase II (35,36) . In addition, this process promotes the union of the specific factor of cytoplasmic polyadenylation (CPSF) with the sequence 5´AAUAAA´3; at the same time allowing the embryonic protein of binding adenine (ePAB) to add adenine to the UTR´3, which causes the UTR´5 to stabilize for the correct translation of the mRNA (37)(38)(39)(40)(41) . In mouse ePAB expression occurs in prophase I and metaphase II oocytes and embryos from 2-4 cells, since it activates the embryonic genome; this expression is suppressed, and due to this is considered a maternal mRNA (42) .…”

Section: Inactivación/activación De Los Rnam Maternosmentioning

confidence: 99%

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

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Changes in total RNA, polyadenylated RNA, and actin mRNA during meiotic maturation of mouse oocytes

Cited by 189 publications

References 30 publications

An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos

An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos

Mammalian oocyte maturation

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

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