Changes in thiol/disulfide homeostasis in patients with chronic kidney disease

Solak, İ̇brahim; Güney, İ̇brahim; Mercan, Seher; Erel, Özcan; Neşeli̇oğlu, Salim; Çetinkaya, Çiğdem Damla; Eryılmaz, Mehmet Ali

doi:10.5455/medscience.2019.08.9168

Cited by 3 publications

(8 citation statements)

References 9 publications

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“…More studies are needed for evaluating its role in early or advanced ccRCC. TDH status was used to evaluate the severity of multiple renal and inflammatory diseases (urolithiasis, obstructive uropathy, urinary tract infections, autosomal dominant polycystic disease, acute renal failure, chronic kidney disease, hemodialysis, peritoneal dialysis, renal transplantation) [ 29 , 30 , 31 , 32 , 34 , 35 , 63 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

“…The balance between the serum levels of thiol-proteins (albumin, cysteine, cysteinyl-glycine, glutathione, homocysteine, γ-glutamyl-cysteine-albumin) and the disulfides plays a protective role in cellular redox homeostasis [ 26 , 29 ]. In the literature, abnormal thiol/disulfide homeostasis was not evaluated in cancer patients, but it was associated with urolithiasis, hemodialysis, peritoneal dialysis, renal transplantation patients, acute renal failure, chronic kidney disease, obstructive uropathy, autosomal dominant polycystic kidney disease, urinary tract infections, and malignancy [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

et al. 2020

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Posttranslational modifications are dynamic enzymatic-mediated processes, regulated in time and space, associated with cancer development. We aimed to evaluate the significance of posttranslational modifications in the pathogenesis of clear cell renal cell carcinoma. The authors developed a prospective, observational study during a period of three years and included 55 patients with localized renal cell carcinoma and 30 heathy subjects. Glycosylation, nitration and carbonylation, thiol-disulfide homeostasis, methylation, phosphorylation and proteolytic cleavage were evaluated in the serum of the evaluated subjects in the present study. Our results showed some characteristics for early ccRCC: high production of cytokines, substrate hypersialylation, induced nitrosative and carbonylic stress, arginine hypermethylation, thiol/disulfide homeostasis (TDH) alteration, the regulatory role of soluble receptors (sRAGE—soluble receptor for advanced glycation end products, sIL-6R—soluble receptor for Interleukin 6) in RAGE and IL-6 signaling, the modulatory effect of TK1—thymidine kinase 1 and TuM2-PK—tumoral pyruvate-kinase 2 in controlling the level of phosphometabolites in neoplastic cells. These data could be the initial point for development of a panel of biomarkers such as total sialic acid, orosomucoids, nitrotyrosine, carbonylic metabolites, Asymmetric Dimethylarginines (ADMA), Symmetric Dimethylarginines (SDMA), and thiol-disulfide equilibrium for early diagnosis of ccRCC. Moreover, they could be considered a specific disease posttranslational modification signature which underlines the transition from early to advanced stages in this neoplasia, and of a therapeutic target in kidney oncogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

et al. 2020

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“…In the CKD group, the levels of thiols, total thiols, and disulphides correlated positively with the albumin concentration, and negatively with the urea concentration, whereas the thiol and total thiol levels were positively correlated with the eGFR and negatively with the creatinine level [ 89 ]. Two other recent studies on adult CKD patients not on HD determined that total plasma thiol, and the (“native”) thiol levels were significantly lower in CKD patients than in healthy controls [ 90 , 91 ]. One study also found a significant reduction in the level of disulphides but no significant difference in the disulphide/thiol, disulphide/total thiol, and thiol/total thiol ratios between the CKD and healthy control groups [ 91 ].…”

Section: P-sh and S -Thiolated Proteins In The Mod...mentioning

confidence: 99%

“…One study also found a significant reduction in the level of disulphides but no significant difference in the disulphide/thiol, disulphide/total thiol, and thiol/total thiol ratios between the CKD and healthy control groups [ 91 ]. The other study found a significant variation in disulphide/thiol and disulphide/total thiol ratios only [ 90 ]. This discrepancy could be explained by the fact that the control group in the latter study had a significantly higher BMI than the CKD group, causing an increase in oxidative stress in the control group with increased levels of disulphides [ 92 ].…”

Section: P-sh and S -Thiolated Proteins In The Mod...mentioning

confidence: 99%

“…The protein thiolation index (PTI, a new biomarker of oxidative stress that is defined as the molar ratio of the sum of all LMM-SH bound to S -thiolated plasma proteins to free P-SH [ 46 , 108 ]) is higher in HD patients than in controls, whereas the concentration of P-SH and that of total plasma thiols are lower in HD patients than in healthy subjects [ 90 , 101 , 105 ] ( Figure 3 ). P-SH and PTI provide different but complementary information.…”

Section: P-sh and S -Thiolated Proteins In Hd Pati...mentioning

confidence: 99%

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Blood Thiol Redox State in Chronic Kidney Disease

Garavaglia

Giustarini

Colombo

et al. 2022

IJMS

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Thiols (sulfhydryl groups) are effective antioxidants that can preserve the correct structure of proteins, and can protect cells and tissues from damage induced by oxidative stress. Abnormal levels of thiols have been measured in the blood of patients with moderate-to-severe chronic kidney disease (CKD) compared to healthy subjects, as well as in end-stage renal disease (ESRD) patients on haemodialysis or peritoneal dialysis. The levels of protein thiols (a measure of the endogenous antioxidant capacity inversely related to protein oxidation) and S-thiolated proteins (mixed disulphides of protein thiols and low molecular mass thiols), and the protein thiolation index (the molar ratio of the S-thiolated proteins to free protein thiols in plasma) have been investigated in the plasma or red blood cells of CKD and ESRD patients as possible biomarkers of oxidative stress. This type of minimally invasive analysis provides valuable information on the redox status of the less-easily accessible tissues and organs, and of the whole organism. This review provides an overview of reversible modifications in protein thiols in the setting of CKD and renal replacement therapy. The evidence suggests that protein thiols, S-thiolated proteins, and the protein thiolation index are promising biomarkers of reversible oxidative stress that could be included in the routine monitoring of CKD and ESRD patients.

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Thiol-Disulfide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Cross-Sectional Cohort Study

Elcioglu,

Koktasoglu,

Ozturk

et al. 2024

Preprint

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Background: Oxidative stress (OS) is implicated in the pathogenesis of various chronic inflammatory diseases, including polycystic kidney disease (PKD). While there are indications of OS involvement in PKD development, conclusive evidence is lacking. This study aimed to investigate Thiol-disulfide homeostasis (TDH), a closely related marker to OS, in PKD patients with different renal functions. Methods: A cross-sectional cohort study included 140 participants divided into four groups: ADPKD with eGFR > 60 ml/min/1.73m2 (PKD), ADPKD with eGFR < 60 ml/min/1.73m2 (PKD_CKD), chronic kidney disease unrelated to diabetes or ADPKD (CKD), and healthy controls (HC). Serum for TDH evaluation was collected after an 8-hour fast. Routine metabolic parameters were assessed, and TDH parameters were determined using the automated photometric Erel method. Results: No significant differences were observed between groups in age, sex, BMI, and smoking, except the PKD group had a significantly higher proportion of males than the other groups. Serum TTHIOL and NTHIOL levels were significantly higher in PKD_CKD compared to CKD (p=0.001 and p<0.001, respectively). Serum disulfide values showed no significant differences between PKD_CKD and CKD (p=0.464) or between PKD and HC (p=0.279). Conclusions: Our study reveals altered OS through TDH in ADPKD patients with varying eGFR levels. Significantly higher TTHIOL and NTHIOL levels were found in ADPKD patients with eGFR <60 ml/min/1.73 m2 compared to non-diabetic CKD patients with similar eGFR. Additionally, a significant positive correlation was identified between TTHIOL levels and eGFR values.

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Changes in thiol/disulfide homeostasis in patients with chronic kidney disease

Cited by 3 publications

References 9 publications

Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

Blood Thiol Redox State in Chronic Kidney Disease

Thiol-Disulfide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Cross-Sectional Cohort Study

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