Changes in the transcriptome of abdominal subcutaneous adipose tissue in response to short-term overfeeding in lean and obese men

Shea, Jennifer; French, Curtis R.; Bishop, Jessica; Martin, Glynn; Roebothan, Barbara; Pace, David; Fitzpatrick, Donald G.; Sun, Guang

doi:10.3945/ajcn.2008.25970

Cited by 38 publications

(41 citation statements)

References 49 publications

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“…Importantly, we technically validated our microarray data on all five analysed genes using qPCR. Additionally, the overlap in SAT gene expression data between our study and previous short-term overfeeding studies further supports the biological significance of our data [18,19]. Nevertheless, small changes in gene expression should be interpreted cautiously.…”

Section: Discussionsupporting

confidence: 84%

“…We found a large overlap in the transcriptional response to overfeeding in SAT between our HFO study and two previous short-term (7-14 days) overfeeding studies (ESM Table 6) [18,19].…”

Section: Changes In Sat Expression In Response To Hfosupporting

confidence: 52%

“…The altered transcriptional activity of SAT after 5 days of HFO included upregulation of numerous genes involved in energy metabolism. Previous short-term overfeeding studies mainly found SAT transcriptional changes in genes involved in lipid [18,19] and carbohydrate metabolism [19]. Despite the excess lipid content of the HFO diet compared with the control diet [16], we found that genes upregulated by HFO were enriched in many (10 out of 15) of the KEGG pathways related to carbohydrate metabolism, but only to a few (4 out of 17) of the KEGG pathways related to lipid metabolism (www.…”

Section: Discussionmentioning

confidence: 61%

“…Excess energy intake has unfavourable effects on whole-body insulin sensitivity independent of changes in body weight in both healthy lean [1,16] and obese diabetic individuals [17]. Additionally, short-and long-term overfeeding may alter adipose tissue histology [18] and gene expression [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Adipose tissue transcriptomics and epigenomics in low birthweight men and controls: role of high-fat overfeeding

et al. 2016

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Aims/hypothesis Individuals who had a low birthweight (LBW) are at an increased risk of insulin resistance and type 2 diabetes when exposed to high-fat overfeeding (HFO). We studied genome-wide mRNA expression and DNA methylation in subcutaneous adipose tissue (SAT) after 5 days of HFO and after a control diet in 40 young men, of whom 16 had LBW. Methods mRNA expression was analysed using Affymetrix Human Gene 1.0 ST arrays and DNA methylation using Illumina 450K BeadChip arrays. Results We found differential DNA methylation at 53 sites in SAT from LBW vs normal birthweight (NBW) men (false discovery rate <5%), including sites in the FADS2 and CPLX1 genes previously associated with type 2 diabetes. When we used reference-free cell mixture adjustments to potentially adjust for cell composition, 4,323 sites had differential methylation in LBW vs NBW men. However, no differences in SAT gene expression levels were identified between LBW and NBW men. In the combined group of all 40 participants, 3,276 genes (16.5%) were differentially expressed in SAT after HFO (false discovery rate <5%) and there was no difference between LBW men and controls. The most strongly upregulated genes were ELOVL6, FADS2 and NNAT; in contrast, INSR, IRS2 and the SLC27A2 fatty acid transporter showed decreased expression after HFO. Interestingly, SLC27A2 expression correlated negatively with diabetes-and obesity-related traits in a replication cohort of 142 individuals. DNA methylation at 652 CpG sites (including in CDK5, IGFBP5 and SLC2A4) was altered in SAT after overfeeding in this and in another cohort. Conclusions/interpretation Young men who had a LBW exhibit epigenetic alterations in their adipose tissue that potentially influence insulin resistance and risk of type 2 diabetes. Short-term overfeeding influences gene transcription and, to some extent, DNA methylation in adipose tissue; there was no major difference in this response between LBW and control participants.

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Section: Discussionsupporting

confidence: 84%

“…We found a large overlap in the transcriptional response to overfeeding in SAT between our HFO study and two previous short-term (7-14 days) overfeeding studies (ESM Table 6) [18,19].…”

Section: Changes In Sat Expression In Response To Hfosupporting

confidence: 52%

Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adipose tissue transcriptomics and epigenomics in low birthweight men and controls: role of high-fat overfeeding

et al. 2016

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“…Our laboratory and others have shown that changes in nutritional status, such as overfeeding, have major effects on adipose tissue metabolism (19,20), circulating concentrations of adipokines (21), and gene expression profiles (22). A positive energy balance may also influence circulating concentrations of gut hormones, such as PYY, and subsequently affect appetite.…”

Section: Introductionmentioning

confidence: 99%

Serum peptide YY in response to short-term overfeeding in young men

Cahill

Shea

Randell

et al. 2011

The American Journal of Clinical Nutrition

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Background: Peptide YY (PYY), a gut hormone that inhibits appetite, has been linked to the development of obesity. Objective: This study investigated the nutritional regulation of PYY after 7 d of overfeeding (70% above normal energy requirements) in normal-weight, overweight, and obese men. Design: Sixty-nine men (aged 19-29 y) participated in the study. We analyzed the relation between fasting serum PYY before and after a 7-d overfeeding challenge in normal-weight, overweight, and obese men. In addition, we analyzed PYY with obesity-related phenotypes including weight, percentage body fat (measured by dual-energy X-ray absorptiometry), body mass index (BMI), total cholesterol, HDL, LDL, glucose, insulin, insulin resistance, and b cell function evaluated by the homeostasis model assessment of insulin resistance (HOMA-IR) and b cell function (HOMA-b) at baseline and in response to the energy surplus. Results: Fasting serum PYY concentrations at baseline were not significantly different between the normal-weight, overweight, and obese subjects on the basis of dual-energy X-ray absorptiometry or BMI. Although the PYY concentration significantly increased due to overfeeding, no differences were observed between adiposity statuses. In addition, basal PYY was negatively correlated with the changes of total cholesterol, HDL, and LDL in normal weight. In addition, the increase in PYY after overfeeding was positively correlated with HDL cholesterol and glucose in normal-weight subjects. Conclusions: Our findings suggest that fasting PYY concentrations are not associated with adiposity status. Moreover, the 7-d overfeeding challenge significantly increased fasting PYY, which is likely a protective response to the positive energy balance.Am J Clin Nutr 2011;93:741-7.

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Short‐term, high fat feeding‐induced changes in white adipose tissue gene expression are highly predictive for long‐term changes

Voigt

Agnew

Schothorst

et al. 2013

Molecular Nutrition Food Res

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Changes in the transcriptome of abdominal subcutaneous adipose tissue in response to short-term overfeeding in lean and obese men

Abstract: Differential expression of these 6 genes may represent a protective mechanism at the molecular level in lean subjects in response to an energy surplus. These genes represent valuable candidates for downstream studies related to obesity.

Cited by 38 publications

References 49 publications

Adipose tissue transcriptomics and epigenomics in low birthweight men and controls: role of high-fat overfeeding

Adipose tissue transcriptomics and epigenomics in low birthweight men and controls: role of high-fat overfeeding

Serum peptide YY in response to short-term overfeeding in young men

Short‐term, high fat feeding‐induced changes in white adipose tissue gene expression are highly predictive for long‐term changes

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