Changes in the Tear Protein Patterns of Diabetic Patients Using Two-Dimensional Electrophoresis.

Human tear proteomics and peptidomics in ophthalmology: Toward the translation of proteomic biomarkers into clinical practice

“…2DE was stablished in the nineties as a powerful technique for protein separation and proteomic downstream analysis, especially applicable for quantitative proteomic comparisons (26). A significant number of studies using 2DE for the differential analysis of tear samples are available (14,16,(27)(28)(29). The specific optimization of the 2D protocol for tear analysis has been reported (30,31).…”

Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors

“…For example, electrophoretic and chromatographic analyses suggest that the tear protein patterns of diabetic patients are very different from those of healthy subjects [4,5]. Biochemical characterization of tear proteins is also important for understanding tear deficiencies, contact lens incompatibilities, tear film instabilities and several other eye diseases.…”

Proteomics in ocular fluids

“…Tears can be eluted from these strips using buffers compatible for further analysis [18]. The minimal invasiveness of the methods of sample collection makes tears a very interesting option for proteomic studies, not only in ocular diseases, but also in other conditions, e.g., diabetes mellitus [19][20][21][22] or cancer [23]. Tear film proteins were extensively analyzed by HPLC [24,25], 2-DE [26,27] and other chromatographic approaches [28].…”