Changes in the Tear Protein Patterns of Diabetic Patients Using Two-Dimensional Electrophoresis

Herber, Simone; Grus, Franz H.; Sabuncuo, Perihan; Augustin, Albert J.

doi:10.1007/978-1-4615-0717-8_88

Cited by 22 publications

(13 citation statements)

References 2 publications

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“…Among the sources for liquid biopsy, tear fluid (TF) representing ocular fluid is not frequently used, but is a good source for non-invasive investigation, as it is easy to assess and clear and it exhibits limited bacterial contamination. Therefore, TF has been used to diagnose various ocular and systemic diseases such as glaucoma 3 , 4 , dry eye disease (DED) 1 , 5 , 6 , thyroid-associated orbitopathy 7 , diabetes mellitus 8 , and cancer 9 – 11 .…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of human lacrimal and tear fluid in dry eye disease

Jung

Hwang

et al. 2017

Sci Rep

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To understand the pathophysiology of dry eye disease (DED), it is necessary to characterize proteins in the ocular surface fluids, including tear fluid (TF) and lacrimal fluid (LF). There have been several reports of TF proteomes, but few proteomic studies have examined LF secreted from the lacrimal gland (LG). Therefore, we characterized the proteins constituting TF and LF by liquid chromatography mass spectrometry. TF and LF were collected from patients with non-Sjögren syndrome DED and from healthy subjects. Through protein profiling and label-free quantification, 1165 proteins from TF and 1448 from LF were identified. In total, 849 proteins were present in both TF and LF. Next, candidate biomarkers were verified using the multiple reaction monitoring assay in both TF and LF of 17 DED patients and 17 healthy controls. As a result, 16 marker proteins were identified (fold-change > 1.5, p-value < 0.05), of which 3 were upregulated in TF and 8 up- and 5 down-regulated in LF. In conclusion, this study revealed novel DED markers originating from the LG and tears by in-depth proteomic analysis and comparison of TF and LF proteins.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of human lacrimal and tear fluid in dry eye disease

Jung

Hwang

et al. 2017

Sci Rep

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“…The tear film covering the surface of the eye is a complex body fluid containing thousands of molecules with different structures and functions [ 3 – 7 ]. A molecular analysis of tear film composition is a useful source of information for the diagnosis, prognosis and treatment of diseases of the eye, as well as systemic diseases in humans [ 8 – 11 ]. In addition to its clinical utility, the identification of biomarkers in tear film may be useful in developing new pharmacologically active molecules and diagnostic tests [ 12 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Dog Tear Film Proteome In-Depth Analysis

Winiarczyk

Banach

et al. 2015

PLoS ONE

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In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the protein components in each fraction. In total, 125 tear proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -protein kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occuring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.

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“…In these studies, the detection of dry-eye syndrome based on the specific electrophoretic patterns was demonstrated to be superior to detection by clinical standard tests (e.g., Schirmer test [BST]) and break-up time [BUT] 8 -10 ). Furthermore, altered protein profiles in tears were found, not only in dry eye, but also in some other ocular and systemic diseases (e.g., diabetes mellitus [11][12][13][14] ) and in cases with other factors that might interfere with the ocular surface (e.g., contact lenses and smoking 15,16 ). All these studies provide evidence that specific proteins or peptides in tear film can be used as diagnostic biomarkers for dry eye, ocular surface diseases, and even systemic disease states such as diabetes mellitus.…”

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confidence: 99%

“…Using this technology, we were able to analyze and quantify single proteins in tear film and to establish a tear film protein map. 11,12,18,20,21 However, the 2-D electrophoretic analysis of proteins is very time consuming and is limited by problems in reproducibility-particularly important when this technique is used for mass-screening of many samples in clinical routine. To eliminate gel-to-gel variation, very complex and tedious procedures to eliminate gel deterioration must be performed to ensure the quality of the spot-matching process.…”

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confidence: 99%