Changes in the surface charge of cells induced by electrical pulses

Томов, Тома; Tsoneva, Iana

doi:10.1016/0022-0728(89)87304-8

Cited by 3 publications

(5 citation statements)

References 19 publications

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“…PEF treatment opens the pores in the cell membranes and releases proteins, ions and other intracellular compounds [Liu et al, 2011], which can change the charge on the cell wall surface and consequently reduce the potential barrier for the influx of TPP þ . The PEF influence on the charge of the yeast cell wall has been shown previously [Tomov and Tsoneva, 1989]. The obtained results confirm the assumption that the influence of the PEF on the yeast cell wall increases its permeability to TPP þ .…”

Section: Discussionsupporting

confidence: 84%

“…The fluorescent probe calcein blue was used to investigate the aqueous pathways suitable for the movement of both ions and uncharged molecules inside and outside the cells. The PEF influence on the charge of the yeast cell wall has been shown previously [Tomov and Tsoneva, 1989]. This also correlates with the accumulation of TPP þ .…”

Section: Discussionsupporting

confidence: 57%

“…These include treatment of the cells chemically by adding reducing agents such as dithiothreitol (DTT) or antibiotics [Brajtburg et al, 1990;Klis et al, 2007]. Various charged molecule adsorption measurements are taken to determine the changes induced in the yeast cell wall surface charge by the PEF [Tomov and Tsoneva, 1989;Neumann et al, 1996]. In order to detect changes of the permeability of the yeast cells, fluorescent probes are used.…”

Section: Introductionmentioning

confidence: 99%

“…In order to detect changes of the permeability of the yeast cells, fluorescent probes are used. Various charged molecule adsorption measurements are taken to determine the changes induced in the yeast cell wall surface charge by the PEF [Tomov and Tsoneva, 1989;Neumann et al, 1996]. Bleomycin has also been used as a probe in yeast viability studies affected by PEF.…”

Section: Introductionmentioning

confidence: 99%

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Electric field‐induced effects on yeast cell wall permeabilization

Stirkė

Zimkus

Ramanavičienė

et al. 2013

Bioelectromagnetics

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The permeability of the yeast cells (Saccharomyces cerevisiae) to lipophilic tetraphenylphosphonium cations (TPP(+) ) after their treatment with single square-shaped strong electric field pulses was analyzed. Pulsed electric fields (PEF) with durations from 5 to 150 µs and strengths from 0 to 10 kV/cm were applied to a standard electroporation cuvette filled with the appropriate buffer. The TPP(+) absorption process was analyzed using an ion selective microelectrode (ISE) and the plasma membrane permeability was determined by measurements obtained using a calcein blue dye release assay. The viability of the yeast and the inactivation of the cells were determined using the optical absorbance method. The experimental data taken after yeasts were treated with PEF and incubated for 3 min showed an increased uptake of TPP(+) by the yeast. This process can be controlled by setting the amplitude and pulse duration of the applied PEF. The kinetics of the TPP(+) absorption process is described using the second order absolute rate equation. It was concluded that the changes of the charge on the yeast cell wall, which is the main barrier for TPP(+) , is due to the poration of the plasma membrane. The applicability of the TPP(+) absorption measurements for the analysis of yeast cells electroporation process is also discussed.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electric field‐induced effects on yeast cell wall permeabilization

Stirkė

Zimkus

Ramanavičienė

et al. 2013

Bioelectromagnetics

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“…When the new membrane electric potential difference ADW 0 F DW i A locally reaches a critical value (DW c ) (nowadays estimated between 0.25 and 0.6 V for living cells [4][5][6][7]), a local alteration of the membrane structure leads to membrane permeabilization. The biological relevance of Eq.…”

Section: Introductionmentioning

confidence: 99%