Changes in the protein concentration and volume of the haemolymph in relation to yolk deposition, ovariectomy, allatectomy, and cautery of the median neurosecretory cells in Melanoplus sanguinipes

Abstract

The protein concentration and volume of the haemolymph may change with no apparent relation to one another in normal, ovariectomized, allatectomized, and median-neurosecretorycell-cauterized (mNSC-cauterized) females. Therefore, protein levels in the haemolymph are more meaningfully expressed in terms of the total protein content. In normal females, fluctuations in the haemolymph volume tend to parallel changes in the protein concentration during the first and subsequent gonotrophic periods. However, significantly less protein accumulates during the latter periods. The suggestion that these fluctuations partly reflect changes in the vitellogenic requirements of the oocytes is supported by the finding that both the volume and protein concentration increase significantly after ovariectomy.Allatectomy or mNSC cautery prevents the normal accumulation of protein in the haemolymph. In allatectomized females, the slight increase in protein concentration is accompanied by a decline in haemolymph volume. Cautery of the mNSC, provided it is performed within 3 h of emergence, results in a low protein concentration but has no effect on the haemolymph volume. The observations are discussed in terms of the corpora allata and mNSC control of haemolymph protein synthesis.

Qualitative and quantitative changes in hemolymph proteins during an ovarian cycle and after insemination in Thermobia domestica (packard) (thysanura, lepismatidae)

“…1). A similar process was observed, for example, in Melanoplus [28], Diplopferu [29], and Curausius [30]. After ecdysis, the protein level increased in T. domesticu and on the fourth day reached a peak of 30.5 pglpl, which was almost the same as that found on the day before ecdysis (Fig.…”

Parasitism of the grasshopper Melanoplus sanguinipes by a sarcophagid fly, Blaesoxipha atlanis: influence of solitary and gregarious development on host and parasitoid

“…Whereas the percentage of total DM allocated to gonadal tissues reached a plateau at 5% in unmated males 6 days after ecdysis, it remained constant at 3% for 10 days after ecdysis in unmated females, increasing to 14% thereafter. Miranpuri et al (1991) reported that the concentration of protein and carbohydrate was less in the hemolymph of males than females, varying up to four-fold with the stage of oocyte development in the latter (Elliott & Gillott, 1977). Host age was also shown to affect parasitoid growth and development in other systems, either because host resources were depleted (Sandlan, 1982) or became unavailable due to host development (Otto & Mackauer, 1998).…”

Identification, characterization, and developmental profile of a high molecular weight, juvenile hormone‐binding protein in the hemolymph of the migratory grasshopper, Melanoplus sanguinipes

“…Adult female M. sanguinipes show relatively high concentrations of JHBP during days 4 to 8, a period coinciding with the time of production and deposition of yolk in the first egg batch, processes strongly influenced by JH (Elliott and Gillott, 1977). In L. maderae (Koeppe et al, 1987) the concentration of JHBP in the hemolymph is stage-dependent with a peak during oocyte maturation suggesting that rates of synthesis of these proteins might be highest during ovarian maturation.…”