Changes in the Profile of Fecal Microbiota and Metabolites as Well as Serum Metabolites and Proteome After Dietary Inulin Supplementation in Dairy Cows With Subclinical Mastitis

Wang, Yue; Nan, Xuemei; Zhao, Yiguang; Jiang, Linshu; Wang, Hui; Zhang, Fan; Hua, Dengke; Li, Jun; Yang, Liguo; Yao, Junhu; Xiong, Benhai

doi:10.3389/fmicb.2022.809139

Cited by 8 publications

(1 citation statement)

References 69 publications

(115 reference statements)

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“…However, despite the relevant role played by bacteria in SM etiology, the milk microbial composition associated to this clinical status is still far from being completely dissected. Indeed, most of publicly available metagenomic studies only employed 16S rRNA gene microbial profiling-based sequencing, thus preventing an accurate and complete characterization of the bovine milk microbiota associated to SM down to the species level (3,6,7,11,12). At the same time, studies limited to culture-dependent investigations, despite being able to identify the presence of underrepresented pathogenic microorganisms in subclinical bovine milk whose detection can escape metagenomics due to the intrinsic limit of this molecular approach (13), do not allow to obtain an accurate overview of how the milk microbiota can change during SM (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of milk microbiota in the bovine subclinical mastitis

Alessandri

Sangalli

Facchi

et al. 2023

Preprint

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Subclinical mastitis is one of the most widespread diseases affecting dairy herds with detrimental effects on animal health as well as on milk productivity and quality. Despite the multi-factorial nature of this intramammary infection, the presence of pathogenic bacteria is regarded one of the main drivers of subclinical mastitis, leading to a disruption of the homeostasis of the bovine milk microbial community. However, the bovine milk microbiota alterations associated with subclinical mastitis still represents a largely unexplored research area. In this context, the species-level milk microbiota of a total of 75 milk samples, collected from both healthy and subclinical mastitis-affected cows from two different stables, was deeply profiled through an ITS, rather than a traditional, and less informative, 16S rRNA gene microbial profiling-based sequencing. Surprisingly, the obtained data of the present pilot study, not only revealed that subclinical mastitis is characterized by a reduced number of species in the bovine milk microbiota, but also that this disease does not induce standard alterations of the milk microbial community across stables. In addition, a flow cytometry-based total bacterial cell enumeration highlighted that subclinical mastitis is accompanied by a significant increment in the number of milk microbial cells. Furthermore, the combination of the metagenomic approach and total bacterial cell enumeration allowed to identify different potential microbial marker strictly correlated with subclinical mastitis across stables.

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Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of milk microbiota in the bovine subclinical mastitis

Alessandri

Sangalli

Facchi

et al. 2023

Preprint

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Nutrition, gastrointestinal microorganisms and metabolites in mastitis occurrence and control

Wang,

Zhao,

Tang

et al. 2024

Animal Nutrition

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Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice

Zhao

Hao

et al. 2023

npj Biofilms Microbiomes

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Although emerging evidence shows that gut microbiota-mediated metabolic changes regulate intestinal pathogen invasions, little is known about whether and how gut microbiota-mediated metabolites affect pathogen infection in the distal organs. In this study, untargeted metabolomics was performed to identify the metabolic changes in a subacute ruminal acidosis (SARA)-associated mastitis model, a mastitis model with increased susceptibility to Staphylococcus aureus (S. aureus). The results showed that cows with SARA had reduced cholic acid (CA) and deoxycholic acid (DCA) levels compared to healthy cows. Treatment of mice with DCA, but not CA, alleviated S. aureus-induced mastitis by improving inflammation and the blood-milk barrier integrity in mice. DCA inhibited the activation of NF-κB and NLRP3 signatures caused by S. aureus in the mouse mammary epithelial cells, which was involved in the activation of TGR5. DCA-mediated TGR5 activation inhibited the NF-κB and NLRP3 pathways and mastitis caused by S. aureus via activating cAMP and PKA. Moreover, gut-dysbiotic mice had impaired TGR5 activation and aggravated S. aureus-induced mastitis, while restoring TGR5 activation by spore-forming bacteria reversed these changes. Furthermore, supplementation of mice with secondary bile acids producer Clostridium scindens also activated TGR5 and alleviated S. aureus-induced mastitis in mice. These results suggest that impaired secondary bile acid production by gut dysbiosis facilitates the development of S. aureus-induced mastitis and highlight a potential strategy for the intervention of distal infection by regulating gut microbial metabolism.

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Changes in the Profile of Fecal Microbiota and Metabolites as Well as Serum Metabolites and Proteome After Dietary Inulin Supplementation in Dairy Cows With Subclinical Mastitis

Abstract: Graphical AbstractEffects of dietary inulin supplementation on fecal microorganisms and metabolites, as well as serum metabolites and proteins in dairy cows with subclinical mastitis.

Cited by 8 publications

References 69 publications

Metagenomic analysis of milk microbiota in the bovine subclinical mastitis

Metagenomic analysis of milk microbiota in the bovine subclinical mastitis

Nutrition, gastrointestinal microorganisms and metabolites in mastitis occurrence and control

Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice

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