Changes in the Pancreas in Experimental Diabetes and the Effect of Lycopene on These Changes: Proliferating, Apoptotic, and Estrogen Receptor α Positive Cells

Abstract

This study aimed to investigate the changes occurring in estrogen receptor (ER) α-positive cells, proliferating cells, apoptotic cells and malondialdehyde (MDA) expression in the pancreas of experimentally induced adult diabetic rats and to determine the effect of orally administered lycopene on these changes. Experimental diabetes was induced using a single dose of 50 mg/kg streptozotocin (STZ). Following the administration of STZ, four groups of animals were established: Control + corn oil, control + lycopene, diabetic + corn oil and diabetic + lycopene. The expressions of ER α, Ki-67, and MDA were determined through immunohistochemistry in sections taken from pancreas tissue samples at the end of the experiment. Apoptotic cells were determined through the TUNEL method. In the diabetic groups, the densities of ER α expression in islets and ER α-positive cells in exocrine parts increased. Whereas the number of proliferating Ki-67 positive cells was higher in the diabetic groups, no significant difference was observed in terms of apoptotic cell number between the control and diabetic groups. Lycopene in general did not have any effect on any of the parameters analyzed in the study. The presence of ER α-positive cells around the islets was demonstrated for the first time in diabetic groups. Based on these observations, demonstrating the antioxidant effects of lycopene in the pancreas of diabetic rats may be possible by increasing the dose and/or the duration of lycopene. Anat Rec, 2017. © 2017 Wiley Periodicals, Inc. Anat Rec, 300:2000-2007, 2017. © 2017 Wiley Periodicals, Inc.

Lycopene ameliorates islet function and down-regulates the TLR4/MyD88/NF-κB pathway in diabetic mice and Min6 cells

“…It should be noted that a study from Sandikci's group revealed that an increase in apoptotic cells was not observed in STZ-induced diabetic rats. 63 One potential explanation for the conflicting findings may be attributed to the different diabetes animal models between these two studies. While STZ was used to obtain hyperglycemia models in a previous investigation, HFD/STZ was used to establish diabetic animal models in our investigation.…”

Female Wistar rats present particular glucose flux when submitted to classic protocols of experimental diabetes

“…Another important factor is the estradiol role in β cells protection against various insults, among which is STZ exposure [ 11 , 29 ]. Also, the α estradiol receptor expression increases in diabetogenic drugs presence, indicating the importance of this defence mechanism [ 46 ]. Therefore, it is likely that even with no observable changes in insulinemia, the classic protocols are able to disrupt, at least partially, insulin secretion in female Wistar rats, and outcome already observed in studies with female mice [ 47 ].…”

INFORMATIVE BIOMARKERS IN THE STUDY OF THE PROCESSES OF PROLIFERATION, APOPTOSIS AND AUTOPHAGY IN THE TISSUES OF THE DIGESTIVE SYSTEM IN EXPERIMENTAL METABOLIC SYNDROME. Review

“…ϳä ÷àñ íàóêîâèõ äîñë³äaeåíü äëÿ îö³íêè ïðî-ë³ôåðàòèâíî¿ àêòèâíîñò³ â îðãàíàõ øëóíêîâî-êèøêîâîãî òðàêòó çà óìîâ ïîðóøåííÿ îáì³íó ÷àñòî çàñòîñîâóºòüñÿ ³ìóíîã³ñòîëîã³÷íå âèçíà÷åííÿ Ki-67. Ç âèêîðèñòàííÿì ö³º¿ ìåòîäèêè áóëî âñòàíîâëåíî, ùî ó ï³äøëóíêîâ³é çàëîç³ äîðîñëèõ ùóð³â, ó ÿêèõ çà äîïîìîãî îäíîðàçîâîãî ââåäåííÿ ñòðåïòîçîòîöèíó ó äîç³ 50 ìã/êã ìàñè ò³ëà ùóð³â âèêëèêàëè ðîçâèòîê åêñïåðèìåíòàëüíîãî ä³àáåòó, ê³ëüê³ñòü Ki-67-ïîçèòèâíèõ ïðîë³ôåðóþ÷èõ êë³òèí â îñòð³âöÿõ âèÿâèëàñÿ á³ëüøîþ, í³ae ó ùóð³â ãðóïè êîíòðîëþ [40].…”