Changes in the Oxidant-Antioxidant Balance in the Kidney of Rats With Nephrolithiasis Induced by Ethylene Glycol

Huang, Huey W.; Chieh, Ming; Chen, Chau Fong

doi:10.1016/s0022-5347(05)65042-2

Cited by 102 publications

(83 citation statements)

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“…Intrarenal oxidative damage and inflammation are common pathological pathways detected in nephrolithic rats. Huang et al [8,9] showed in nephrolithic rats that free radicals were generated at an early stage and persisted, while the infiltration of leukocytes (CD45-positive cells) was progressively increased at later stages. de Water et al [5,6] showed that interstitial crystals were mostly surrounded by ED1-positive cells (specific for monocytes and macrophages) and multinucleated giant cells.…”

Section: Discussionmentioning

confidence: 99%

“…These cells function to encapsulate and dissolve the crystals [5][6][7]. A study in ethylene glycolinduced nephrolithic rats showed that free radicals were increasingly generated at an early stage and persisted in the kidney, whilst the infiltration of leukocytes gradually increased in the late stage of stone formation [8,9]. Even though the underlying mechanism of leukocyte infiltration is not known, it is likely that renal tubular cells affected by lithogenic crystals produce chemotactic factors such as monocyte chemoattractant protein-1 (MCP-1).…”

Section: Introductionmentioning

confidence: 99%

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Messenger RNA expression of monocyte chemoattractant protein‐1 and interleukin‐6 in stone‐containing kidneys

et al. 2008

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OBJECTIVESTo investigate the intrarenal mRNA expression of monocyte chemoattractant protein‐1 (MCP‐1) and interleukin‐6 (IL‐6) in patients with nephrolithiasis, and to evaluate whether their expression is associated with renal function, as oxidative stress and inflammation are involved in the pathogenesis of nephrolithiasis.PATIENTS, SUBJECTS AND METHODSRenal biopsies from near the stone, and blood and 24‐h urine specimens were collected from 29 patients with nephrolithiasis. Control renal tissues were taken from non‐cancerous and cancerous portions of nephrectomy from six patients with renal cancers, and control 24‐h urine samples were obtained from 30 healthy subjects. Corrected creatinine clearance, urinary N‐acetyl‐β‐glucosaminidase activity and 8‐hydroxy‐deoxyguanosine (8‐OHdG) were determined. The mRNA expressions of MCP‐1 and IL‐6 in the tissues were measured by real time reverse transcription‐polymerase chain reaction.RESULTSPatients with nephrolithiasis had significantly greater renal tubular damage and oxidative stress than the healthy controls. Intrarenal mRNA expressions of MCP‐1 and IL‐6 in stone‐adjacent renal tissues were significantly lower than in cancerous renal tissues, but not statistically different from that in non‐cancerous renal tissues. In stone‐adjacent renal tissues, the mRNA level of MCP‐1 was significantly higher than that of IL‐6, but their expressions were significantly correlated with each other. Histological examination showed that the number of infiltrated leukocytes corresponded well with the intrarenal mRNA levels of MCP‐1 and IL‐6. Patients with nephrolithiasis and compromised renal function had significantly higher intrarenal mRNA levels of MCP‐1 and IL‐6 than those with preserved renal function. Also, the mRNA levels in patients with severe renal tubular damage were significantly greater than in those with less renal tubular damage. There was no association between intrarenal mRNA expression and urinary 8‐OHdG.CONCLUSIONNephrolithiasis was associated with low‐grade intrarenal inflammation. A greater intrarenal mRNA expression of MCP‐1 and IL‐6 was associated with enhanced renal impairment. Thus, expression of MCP‐1 and IL‐6, at least in part, contributed to the progression of nephrolithiasis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Messenger RNA expression of monocyte chemoattractant protein‐1 and interleukin‐6 in stone‐containing kidneys

et al. 2008

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“…The principal causative agent for the formation of calcium salt crystals is thought to be supersaturation that causes the precipitation of salts in urine. However, even though urine is supersaturated with calcium and oxalate ions in normal subjects, stones do not form because of the presence of inhibitors and other unknown mechanisms (22).Membrane injury is suggested to be the primary event in CaOx crystal binding, and oxalate-induced free radicals are significant causative factors in membrane injury (11,12,25). This injury leads to lipid and protein peroxidation and altered biochemical reactions, including depletion of the antioxidant defense system (25).…”

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confidence: 99%

“…Membrane injury is suggested to be the primary event in CaOx crystal binding, and oxalate-induced free radicals are significant causative factors in membrane injury (11,12,25). This injury leads to lipid and protein peroxidation and altered biochemical reactions, including depletion of the antioxidant defense system (25).…”

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confidence: 99%

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Low-vitamin E diet exacerbates calcium oxalate crystal formation via enhanced oxidative stress in rat hyperoxaluric kidney

Huang¹,

Chieh²

2009

American Journal of Physiology-Renal Physiology

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Huang HS, Ma MC, Chen J. Low-vitamin E diet exacerbates calcium oxalate crystal formation via enhanced oxidative stress in rat hyperoxaluric kidney. Am J Physiol Renal Physiol 295: F34-F45, 2008. First published September 17, 2008 doi:10.1152/ajprenal.90309.2008.-Vitamin E was previously reported to reduce calcium oxalate (CaOx) crystal formation. This study explored whether vitamin E deficiency affects intrarenal oxidative stress and accelerates crystal deposition in hyperoxaluria. The control (C) group of rats received a standard diet and drinking water, while the experimental groups received 0.75% ethylene glycol (EG) in drinking water for 42 days. Of the latter, one group received a standard diet (EG group), one received a low-vitamin E (LE) diet (EGϩLE group), and the last received an LE diet with vitamin E supplement (4 mg) (EGϩLEϩE group). The CϩLE and CϩLEϩE groups were the specific controls for the last two experimental groups, respectively. In a separate experiment, EG and EGϩLE rats were studied on days 3-42 to examine the temporal relationship between oxidative change and crystal formation. Urinary biochemistry and activity/levels of antioxidative and oxidative enzymes in glomeruli and tubulointerstitial specimens (TIS) were examined. In EG rats, CaOx crystal accumulation was associated with low antioxidative enzyme activity in TIS and with increased oxidative enzyme expression in glomeruli. In the EGϩLE group, marked changes in antioxidative and oxidative enzyme levels were seen and correlated with massive CaOx deposition and tubular damage. The increased oxidative stress seen with EGϩLE treatment was largely reversed by vitamin E supplementation. A temporal study showed that decrease in antioxidative defense and increased free radical formation in the EGϩLE group occurred before crystal deposition. This study shows that low vitamin E disrupts the redox balance and causes cell death, thereby favoring crystal formation. antioxidative proteins; free radicals; hyperoxaluria CALCIUM OXALATE (CaOx) crystal formation is a multifaceted and complex process that involves a series of chemical, physical, biochemical, and physiological events (6, 10, 16). The principal causative agent for the formation of calcium salt crystals is thought to be supersaturation that causes the precipitation of salts in urine. However, even though urine is supersaturated with calcium and oxalate ions in normal subjects, stones do not form because of the presence of inhibitors and other unknown mechanisms (22).Membrane injury is suggested to be the primary event in CaOx crystal binding, and oxalate-induced free radicals are significant causative factors in membrane injury (11,12,25). This injury leads to lipid and protein peroxidation and altered biochemical reactions, including depletion of the antioxidant defense system (25). On the basis of these findings, we hypothesized that if renal tubular injury due to increased oxidative stress caused by hyperoxaluria can be prevented, CaOx crystal accumulation in the kidneys can also be ...

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Quercus salicina Blume: Research Progress in Chemistry and Pharmacodynamics (1959–2021)

Yue

Yin

Ding

et al. 2022

Chemistry & Biodiversity

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The crude extracts of different parts (leaves and shoots) of Quercus salicina Blume (QS) have shown considerable effect in urolithiasis. QS has been widely used in clinical practice and has attracted great research interest The relevant published literature, however, reveals only partial education of its chemical components and bio-active mechanisms, and only two review articles have summarized the QS research progress. In this review, a comprehensive and systematic review of chemistry and pharmacodynamics of QS was carried out using the international authoritative databases (1959-2021), focusing on phenols and flavonoids, and their effect such as urinary stone dissolution, anti-inflammatory, anti-diabetes, anti-bacterial, antioxidant, and anti-allergy activities as well as toxic effects. The aim of review is to provide the most recent and effective literature support for further basic research and application development.

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Changes in the Oxidant-Antioxidant Balance in the Kidney of Rats With Nephrolithiasis Induced by Ethylene Glycol

Cited by 102 publications

References 29 publications

Messenger RNA expression of monocyte chemoattractant protein‐1 and interleukin‐6 in stone‐containing kidneys

Messenger RNA expression of monocyte chemoattractant protein‐1 and interleukin‐6 in stone‐containing kidneys

Low-vitamin E diet exacerbates calcium oxalate crystal formation via enhanced oxidative stress in rat hyperoxaluric kidney

Quercus salicina Blume: Research Progress in Chemistry and Pharmacodynamics (1959–2021)

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