Changes in the nociceptive response to thermal stimulation in rats following administration of N-terminal analogs of the adrenocorticotropic hormone

Dodonova, Svetlana A.; Bobyntsev, I. I.; Белых, А. Е.; Andreeva, L. A.; Myasoedov, N. F.

doi:10.24075/brsmu.2019.085

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Присоединение трипептида Pro-Gly-Pro (ПГП) к С-концу данной молекулы повышает ее устойчивость к действию карбоксипептидаз. Так, АКТГ 6-9 -ПГП обладает широким спектром нейротропных эффектов и в сопоставимых дозах проявляет более выраженную, чем АКТГ 4-7 -ПГП, активность в отношении тревожных и депрессивных реакций [8,9], выработки и консолидации условных рефлексов [10], болевой чувствительности [11].…”

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Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress

et al. 2023

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Aim. To study the morphofunctional state of the colonic wall in rats when using the N–terminal analog of the adrenocorticotropic hormone (ACTH) ACTH6-9-Pro-Gly-Pro (ACTH6-9-PGP) peptide under chronic stress.Materials and methods. The study was performed on 55 male Wistar rats, which were divided into 5 groups (n = 11): group 1 – control group (administration of saline solution without stress); group 2 – chronic restraint stress (CRS) + administration of saline solution; group 3 – CRS + administration of ACTH6-9-PGP at a dose of 5 μg / kg; group 4 – administration of ACTH6-9-PGP at a dose of 50 μg / kg; group 5 – administration of ACTH6-9- PGP at a dose of 500 μg / kg. A histologic examination of the rat colon was performed. The histologic architecture of the colonic wall, the depth of crypts, and the number of goblet cells were assessed. Furthermore, the number of granulocytes, plasma cells, lymphocytes, macrophages, and mast cells was counted.Results. The study demonstrated that chronic (14 days) restraint stress resulted in the development of inflammations in the colonic wall of the animals. Intraperitoneal administration of ACTH6-9-PGP at doses of 50 and 500 μg / kg daily throughout the entire time of stress exposure prevented the development of stress-induced alterations observed in the control animals. At the same time, anti-inflammatory effects of the peptide in the colonic wall and a decrease in the level of corticosterone in the blood serum were noted.Conclusion. The results of this work and data from other studies on the effects of N-terminal analogs of ACTH indicate the need for studying the mechanisms of their effect on inflammation and searching for targets of ACTH6-9-PGP.

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Section: Introductionunclassified

Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress

et al. 2023

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ACTH(6-9)-Pro-Gly-Pro ameliorates anxiety-like and depressive-like behaviour and gut mucosal microbiota composition in rats under conditions of chronic restraint stress

et al. 2022

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Effect of Acth6-9-Pro-Gly-Pro Peptide on Spectral Parameters of Heart Rate Variability in Wistar Rats During Physical Exertion (Pilot Study)

Korobova¹,

Vorvul²,

Bobyntsev³

2023

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Objective: To study the effect of the ACTH6-9-Pro-Gly-Pro peptide (ACTH6-9-PGP) on the spectral parameters of heart rate variability (HRV) in Wistar rats during short-term exercise. Methods: The study involved 48 Wistar rats. ACTH6-9-PGP was once administered to the animals intraperitoneally at doses of 5, 50, and 500 µg/kg (3 groups of animals) in a volume of 1 ml/kg. Animals of the control group were injected with normal saline in an equivalent volume. Each group included 6 males and 6 females. HRV indicators were recorded using the Physiobelt 2.5.1 complex (Neurobotics, Russia). HRV analysis was performed by spectral parameters: total power of the spectrum of HRV (TP), the total power of the high-frequency component (HF) (ms2 , %), the total power of the lowfrequency component (LF) (ms2 , %), the total power of the very low-frequency component (VLF) (ms2 , %), LF/HF ratio (LF/HF), index of centralization (IC). The cardio signal was recorded 4 times: 1 – after adaptation to the device; 2 – 15 minutes after the injection of the peptide; 3 – after physical activity (2-minute treadmill run), and 4 – after a 15-minute rest. Results: An analysis of the initial values of the HRV indicators made it possible to establish a shift in the spectral characteristics toward the VLF component in the studied groups. Physical activity in the control group caused an increase in the power of HF, LF, and VLF, however, their ratio (VLF>LF>HF) did not change at all stages of the study. A single intraperitoneal injection of the ACTH6-9-PGP peptide at a dose of 5 μg/kg stabilized the HRV parameters at the initial level. The administration of the peptide at a dose of 50 μg/kg and moderate physical activity were accompanied by an increase in the power of HF and LF compared to the initial level. The percentage of frequency components after the exercise was LF>VLF>HF, and in the recovery period, it became VLF>LF>HF. After the administration of the peptide at a dose of 500 μg/kg, an increase in HF and LF, and a redistribution of the frequency components toward LF>HF>VLF were noted. During the recovery period, a decrease in HF, restoration of the percentage of frequency indicators VLF>LF>HF, and an increase in LF/HF and IC were found. Conclusion: A study of the effects of the ACTH6-9-PGP peptide on HRV in Wistar rats showed that a single intraperitoneal injection at a dose of 5 µg/kg promotes adaptation of laboratory animals to physical activity due to the activation of suprasegmental structures, and at doses of 50 and 500 µg/kg – predominant activation of the peripheral segments of the sympathetic and parasympathetic autonomic systems. Keywords: ACTH6-9-Pro-Gly-Pro, heart rate variability, physical activity, Wistar rats, spectral parameters.

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Changes in the nociceptive response to thermal stimulation in rats following administration of N-terminal analogs of the adrenocorticotropic hormone

Cited by 3 publications

References 10 publications

Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress

Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress

ACTH(6-9)-Pro-Gly-Pro ameliorates anxiety-like and depressive-like behaviour and gut mucosal microbiota composition in rats under conditions of chronic restraint stress

Effect of Acth6-9-Pro-Gly-Pro Peptide on Spectral Parameters of Heart Rate Variability in Wistar Rats During Physical Exertion (Pilot Study)

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