Changes in the Morphological and Immunological Characteristics of Mel Cher Melanoma Cells in Response to Low Concentration of Embryo Calf Serum

Abstract

Background. Human melanoma cell line mel Cher have a different cell forms and is appraised for grade of differentiation as low-diffe-rentiation melanoma. Objective. The purpose of this study was development and characterization of subclone mel Cher 5C. Materials and methods. For received subclone mel Cher 5C was applied method of colony formation in conditions with low concentration of embryo calf serum (5 %). Then was carry out selection spindle-like cells, their long cultivation and estimate morphological and immunological characteristics. Results. Subclone mel Cher 5C cells differed for morphology from primary line mel Cher. This cells had spindle-like form and had capacity to form spherical type colony on the surface of monolayer during their growth. Subclone cells has more intensive growth compare with cells of primary line. Subclone cells had differences by immunological phenotype and didn’t expressed antigene HLA-DR. Conclusions. Subclone cells had a fusiform shape, in the growth process of the formed spheroid colonies monomorphic type, did not Express antigens of histocompatibility complex II.

Cell lines of human melanoma and their xenograft with braf or nras mutations a targets for targeted therapy. Reviews

“…Около 1 % клеток в монослое имели веретеновидную форму. При культивировании в в чашках D = 60 мм и в среде с 5 % сывороткой при редком посеве клеток (около 200 клеток на чашку), выросшие колонии включали 3 морфологических типа клеток: эпителиоподобные, веретеновидные и смешанного типа [28].…”

“…е.) против 2,02 о. е., а уровень виментина -1,77 о. е. против 1,61 о. е. В среде с 10 % сывороткой уровни экспрессии указанных маркеров в клетках этого субклона были существенно выше, Обзоры литературы а уровень виментина превышал уровень Е-кадгерина: 9,52 о. е. против 3,84 о. е. для E-кадгерина [28]. Иммунологический фенотип клеток субклона mel Ibr EE оказался беднее, чем в исходной линии.…”