Changes in the level of acetylcholinesterase of Nematospiroides dubius and Trichostrongylus colubriformis following paralysis by levamisole in vivo

Sharpe, M. J.; Lee, Donald L.

doi:10.1016/0166-6851(81)90077-3

Cited by 9 publications

(8 citation statements)

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“…Studies in vivo and in vitro on N. brasiliensis and T. colubriformis have determined that females ingested more of the fluorescent dye rhodamine B than males (BOTTJER & BONE, 1984, 1985a. A higher acetylcholinesterase level (SHARPE & LEE, 1981) and acetylcholinesterase secretion (MALLET, 1989) have been observed in male H. polygyrus compared to females. Acetylcholinesterase contained in H. polygyrus has been thought to be primarily associated with the neuromuscular system (OGILVIE et al, 1973) which is known to be affected by levamisole.…”

Section: Discussionmentioning

confidence: 99%

The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

Mallet¹,

Kerbœuf²

1993

J. Helminthol.

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The inhibition of acetylcholinesterase secretion by male and female Heligmosomoides polygyrus was tested on worms taken from experimentally infected mice and maintained for 3 days in vitro in levamisole. The dose inhibiting 50% of enzyme secretion (ID 50) of male worms was twice the ID 50 for female worms. A similar difference was observed in vivo between the dose of levamisole removing 50% (LD 50) of male and female worms from the mouse. Acetylcholinesterase secretion by worms and ID 50 were tested in vitro at 3-weekly intervals from 3 to 21 weeks post infection (WPI). Acetylcholinesterase secretion was always significantly higher for male than for female worms. A decrease of ID 50, correlated with the age of the worms was observed: from 1·5 to 0·5 μg/ml for males and from 0·7 to 0·1 μg/ml for females. These results were confirmed in vivo by a higher efficacy of the anthelminthic at 21 than at 4 WPI.

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Section: Discussionmentioning

confidence: 99%

The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

Mallet¹,

Kerbœuf²

1993

J. Helminthol.

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“…The energy charge is a measure of the energy status of cells, tissue or organism, and varies between 0 and 1 (Atkinson & Walton, 1967). According to Sharpe (1980) the energy charge of most metabolically active cells is maintained between 0-75 and 0-90. A fall below about 0-5 seems to be indicative of an inhibition of oxidative phosphorylation.…”

Section: Schistosoma Mansonimentioning

confidence: 99%

How anthelmintics help us to understand helminths

Bossche

1985

Parasitology

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Study of the mode of action of the an ti-parasitic ' hydrogenophore', closantel, revealed that mitochondria isolated from livers of untreated rats, 13 weeks after infection with Fasciola hepatica metacercariae, were uncoupled. This uncoupling of mitochondria might be induced by a product(s) excreted by the liver fluke. The lipid extractable product(s) binds to albumin and has a molecular weight between 500 and 1000 Daltons. A second unexpected finding indicates that closantel affects the motility of Schistosoma mansoni in vitro by interfering with mitochondrial ATP synthesis. The results obtained are suggestive of a role for aerobic metabolism in the generation of energy required for motility. 23-2

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“…The energy charge has been used in previous studies on parasitic helminths to measure changes in the metabolic state of the parasites (Barrett & Beis, 1973;Beis & Barrett, 1975;Bryant & Behm, 1976;Bryant, Cornish & Rahman, 1976;Richards, Bryant, Kelly, Windon & Dineen, 1977;Ballantyne, Sharpe & Lee, 1978;Sharpe, 1980). The question of the importance of oxygen for the metabolism of parasitic nematodes has been much debated.…”

Section: Introductionmentioning

confidence: 99%

“…(Schottler, 1978). Following paralysis by the anthelmintic levamisole in vivo the energy charge of Nematospiroides dubius and Tricho-f strongylus colubriformis was depressed and this was most apparent in T. \ colubriformis (Sharpe, 1980). However, the changes in energy charge were not of f the order which would be expected if the paralysing action of levamisole were directly affecting the metabolism of ATP in these two species and it was suggested that lack of oxygen, resulting from the change in the environment of the parasites, was the reason for the changes in energy charge (Sharpe, 1980).…”

Section: Introductionmentioning

confidence: 99%

“…Following paralysis by the anthelmintic levamisole in vivo the energy charge of Nematospiroides dubius and Tricho-f strongylus colubriformis was depressed and this was most apparent in T. \ colubriformis (Sharpe, 1980). However, the changes in energy charge were not of f the order which would be expected if the paralysing action of levamisole were directly affecting the metabolism of ATP in these two species and it was suggested that lack of oxygen, resulting from the change in the environment of the parasites, was the reason for the changes in energy charge (Sharpe, 1980). In order to f investigate this, and as one part of a study of the importance of oxygen to the ) metabolism of parasitic nematodes, it was of interest to measure changes in the [ adenylate energy charge of these two parasites maintained in oxygen-deficient conditions compared to parasites maintained in oxygen-rich conditions.…”

Section: Introductionmentioning

confidence: 99%

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The effect of anaerobiosis on adenosine nucleotide levels inNematospiroides dubiusandTrichostrongylus colubriformis in vitro

Sharpe

Lee

1981

Parasitology

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SUMMARYThe adenosine nucleotide and adenylate energy charge values ofNematospiroides dubiusandTrichostrongylus colubriformismaintainedin vitrounder aerated and under near-anoxic conditions have been measured. Maintenance of the nematodes in both conditions had an adverse effect on their energy metabolism, but in comparing these effects it was found that the changes were more significant in those nematodes maintained in near-anoxic conditions and that the changes were more apparent in male nematodes. The smaller changes in the energy charge values of female worms, particularlyN. dubiuswhere there was no difference between worms in aerated conditions and those in near-anoxic conditions, were explained by the presence of eggs in the nematodes. At no time did the energy charge of nematodes in near-anoxic conditions fall below 0·50 but, whereas nematodes in aerated conditions were able to continue moving throughout the experiment, those in near-anoxic conditions stopped moving during the first 3 h. It is suggested that in the absence of sufficient oxygen the nematodes were able to maintain their energy charge above 0·50 by removal of AMP from the adenylate pool, resulting in the depletion of their total adenylate. The changes in the energy charge and behaviour of the nematodes are related to the survival of the nematodes in the oxygen-deficient environment which they inhabitin vivo.

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Changes in the level of acetylcholinesterase of Nematospiroides dubius and Trichostrongylus colubriformis following paralysis by levamisole in vivo

Cited by 9 publications

References 5 publications

The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

How anthelmintics help us to understand helminths

The effect of anaerobiosis on adenosine nucleotide levels inNematospiroides dubiusandTrichostrongylus colubriformis in vitro

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