Changes in the immunohistochemical localization of estrogen receptor alpha and in the stereological parameters of the testes of mature and aged chickens (Gallus domesticus)

González-Morán, María Genoveva

doi:10.1016/j.bbrc.2019.01.097

Cited by 8 publications

(7 citation statements)

References 32 publications

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“…The authors hypothesized that excessive doses of estrogens could disrupt testis physiology, therefore a downregulation of ERs protein by E 2 as a physiological mechanism decreasing the sensitivity of testicular tissue to estrogens has been proposed for gander testes during the breeding season, when the intratesticular E 2 concentration was very high [16]. A positive correlation between ERα protein expression and spermatogenic activity was reported in the testes of immature, mature, as well as aged chickens [13,51]. Furthermore, in quail, a decrease in both oxidative stress-induced plasma estradiol and ERα testicular expression was reported to reduce spermatogenesis [15].…”

Section: Discussionmentioning

confidence: 99%

“…Earlier studies have reported the mRNA expression of testicular ERs in the embryos of chicken [7,8] and duck [9], and the adults of gander [10] and Japanese quail [11,12]. The immunoreactivity of ERs has been detected in the testes of chickens [13,14], Japanese quail [15,16], and domestic goose [17]. Meanwhile, a seasonal pattern of ER expression and E2 concentration has only been demonstrated in male domestic goose [17].…”

Section: Introductionmentioning

confidence: 99%

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Proliferative and Apoptotic Pathways in the Testis of Quail Coturnix coturnix during the Seasonal Reproductive Cycle

Falvo

Rosati

Fiore

et al. 2021

Animals

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The quail Coturnix coturnix is a seasonal breeding species, with the annual reproductive cycle of its testes comprising an activation phase and a regression phase. Our previous results have proven that the testicular levels of both 17β-estradiol (E2) and androgens are higher during the reproductive period compared to the non-reproductive period, which led us to hypothesize that estrogens and androgens may act synergistically to initiate spermatogenesis. The present study was, therefore, aimed to investigate the estrogen responsive system in quail testis in relation to the reproduction seasonality, with a focus on the molecular pathways elicited in both active and regressive quail testes. Western blotting and immunohistochemistry analysis revealed that the expression of ERα, which is the predominant form of estrogen receptors in quail testis, was correlated with E2 concentration, suggesting that increased levels of E2-induced ERα could play a key role in the resumption of spermatogenesis during the reproductive period, when both PCNA and SYCP3, the mitotic and meiotic markers, respectively, were also increased. In the reproductive period we also found the activation of the ERK1/2 and Akt-1 kinase pathways and an increase in second messengers cAMP and cGMP levels. In the non-reproductive phase, when the E2/ERα levels were low, the inactivation of ERK1/2 and Akt-1 pathways favored apoptotic events due to an increase in the levels of Bax and cytochrome C, with a consequent regression of the gonad.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proliferative and Apoptotic Pathways in the Testis of Quail Coturnix coturnix during the Seasonal Reproductive Cycle

Falvo

Rosati

Fiore

et al. 2021

Animals

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“…Some literature data indicate that the expression of estrogen receptors is greater in female than in male gonads already on day 10 of embryogenesis [48]. Due to the fact that sexual differentiation starts on around the 5th day of chicken embryogenesis [49], the increase in ESR1 expression in the ovary stimulates the division of cells in the granulosa layer to increase their number [50]. On the other hand, Yamamoto et al [51] showed a similar level of ESR1 mRNA expression in male and female chicken gonads between 5.5 and 8.5 days of embryogenesis, indicating that they are bipotential at the beginning of embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

Sodium Fluoride In Vitro Treatment Affects the Expression of Gonadotropin and Steroid Hormone Receptors in Chicken Embryonic Gonads

Grzegorzewska¹,

Grot²,

Sechman³

2021

Animals

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Sodium fluoride (NaF), in addition to preventing dental decay may negatively affect the body. The aim of this study was to examine the effect of a 6 h in vitro treatment of gonads isolated from 14-day-old chicken embryos with NaF at doses of 1.7 (D1), 3.5 (D2), 7.1 (D3), and 14.2 mM (D4). The mRNA expression of luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), estrogen receptors (ESR1 and ESR2), progesterone receptor (PGR), and the immunolocalization of progesterone receptors were examined in the tissue. In the ovary, the expression of FSHR and LHR increased following the NaF treatment. In the case of FSHR the highest stimulatory effect was noticed in the D2 group, while the expression of LHR increased in a dose-dependent manner. A gradual increase in ESR1 and PGR mRNA levels was also observed in the ovary following the NaF treatment, but only up to the D3 dose of NaF. The highest ESR2 level was also found in the D3 group. In the testes, the lowest dose of NaF significantly decreased the expression of FSHR, ESR1, ESR2, and PGR. On the other hand, an increase in PGR expression was observed in the D3 group. The expression of LHR in the testes was not affected by the NaF treatment. Immunohistochemical analysis showed that NaF exposure increased progesterone receptor expression in the ovarian cortex, while it decreased its expression in the testes. These results reveal that NaF may disturb the chicken embryonic development and different mechanisms of this toxicant action exist within the females and males.

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“…The effects of estrogens on cells are mediated by the intracellular estrogen receptors α (ERα, estrogen receptor α) and β (ERβ, estrogen receptor β) (20). Studies investigating the role of sex hormones and androgen receptor expression in turkey testes, and describing the agedependent distribution of estrogen receptors in rooster testes, demonstrate the importance of steroid hormones in the regulation of the gonadal function in birds and indicate that hormone-receptor interaction may be an important factor in maintaining spermatogenesis and normal male fertility (15,29). In addition, a study on domestic goose testes showed a reproductive seasondependent expression pattern for ERα and ERβ.…”

mentioning

confidence: 97%

Diverse effect of in ovo treatment with metaloestrogen: selenium on chick testis histology and estrogen signaling

PARDYAK¹,

CHMURSKA-GASOWSKA²,

JASZCZA³

et al. 2022

Medycyna Weterynaryjna

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With the observed in the last years increasing disruption of poultry males’ fertility, there is a need to improve knowledge on the morphology and functioning of the avian reproductive system. A number of molecular and endocrine interrelationships indicate that testicular dysfunction may be one of the reasons for the production of poor semen quality. Because selenium (Se) is known to be an essential element for spermatogenesis, thus the aim of the present study was to determine the effect of Se treatment in ovo on cellular and molecular status (expression of aromatase, estrogen receptors and estrogen level) of testes of chicks. The study was performed on chick embryons exposed to Se at a dose of 0.6 µg/egg. We revealed diverse changes in testis histology after Se-treatment including developmental changes reflected by cell migration or vacuolization of the marginal stroma. We showed for the first time the expression of GPER in the testes of chicks and changes in the expression of aromatase, GPER, ERα and ERβ after treatment with Se. Simultaneously with these, decreased testicular estradiol concentration indicated an altered receptor protein and/or binding by Se only to GPER, ERα that in turn affects hormone concentration and action. Further studies are needed to evaluate other effects of Se supplementation especially of immature birds or mature ones and eggs laying by them per se.

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Changes in the immunohistochemical localization of estrogen receptor alpha and in the stereological parameters of the testes of mature and aged chickens (Gallus domesticus)

Cited by 8 publications

References 32 publications

Proliferative and Apoptotic Pathways in the Testis of Quail Coturnix coturnix during the Seasonal Reproductive Cycle

Proliferative and Apoptotic Pathways in the Testis of Quail Coturnix coturnix during the Seasonal Reproductive Cycle

Sodium Fluoride In Vitro Treatment Affects the Expression of Gonadotropin and Steroid Hormone Receptors in Chicken Embryonic Gonads

Diverse effect of in ovo treatment with metaloestrogen: selenium on chick testis histology and estrogen signaling

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