Changes in the higher order organization of DNA during aging of human fibroblast-like cells

Dell’Orco, Robert T.; Whittle, W.L.; Macieira‐Coelho, A.

doi:10.1016/0047-6374(86)90010-2

Cited by 21 publications

(3 citation statements)

References 31 publications

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“…Therefore, the histone variant synthesis data presented here support the hypothesis that high PDL cells can be stimulated to move through the G1 phase of the cell cycle, but once they are unable to enter S phase, they revert back to a GO state of the cell cycle (Kihara et al, 1986). Age-related alterations in chromatin structure have been detected in HDF as they move through their in vitro lifespan (Puvion-Dutilleul and Macieira Coelho, 1982;Dell'Orco et al, 1986;Gorman and Cristofalo, 1986;Ishimi et al, 1987). Histone variants can affect chromatin structure (Simpson, 19811, and this can alter cellular function and genetic expression (Butler, 1983).…”

Section: Discussionsupporting

confidence: 78%

The effects of in vitro age and culture state on histone variant synthesis in human diploid fibroblasts

Dell’Orco

Worthington

1988

Journal Cellular Physiology

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Histone variant synthesis patterns from human diploid fibroblast-like cells of different in vitro ages were determined during exponential growth, at confluence, and during low serum arrest. The results are reported as the ratios of H2A variant synthesis (H2A.1 and H2A.2/H2A.x and H2A.z) and H3 variant synthesis (H3.1 and H3.2/H3.3) that have been used to characterize individual cell cycle states. Hydroxyurea was employed in some experiments to reduce S phase cells. The results indicate that high population doubling level (PDL) cells move through the G1 phase of the division cycle during exponential growth and exist in the G0 cell cycle state at confluence and during low serum arrest. Low PDL cells, however, exist in the G1 cell cycle state at confluence and revert to a G0 state only after maintenance as quiescent populations. This would suggest that when stimulated high PDL cells cannot enter into S phase, they revert to a GO cell cycle state.

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Section: Discussionsupporting

confidence: 78%

The effects of in vitro age and culture state on histone variant synthesis in human diploid fibroblasts

Dell’Orco

Worthington

1988

Journal Cellular Physiology

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“…These observations suggest the important roles of nucleosomes in the regulation of cellular senescence. Indeed, it should be noted that senescent cells show altered nucleosome/ chromatin organization and increased sensitivity of chromatin to nuclease digestion [22,23,43,44]. Furthermore, decreased histones on chromatin are observed in senescent human and yeast cells [45][46][47][48][49]; and interestingly, the N-terminal tail of histone H3 is cleaved in senescent cells and expression of the cleaved form of Histone H3 is sufficient for induction of cellular senescence in human cells [50].…”

Section: Discussionmentioning

confidence: 99%

The key role of a basic domain of histone H2B N‐terminal tail in the action of 5‐bromodeoxyuridine to induce cellular senescence

Watanabe

Ayusawa

et al. 2022

The FEBS Journal

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5‐Bromodeoxyuridine (BrdU), a thymidine analogue, is an interesting reagent that modulates various biological phenomena. BrdU, upon incorporation into DNA, causes destabilized nucleosome positioning which leads to changes in heterochromatin organization and gene expression in cells. We have previously shown that BrdU effectively induces cellular senescence, a phenomenon of irreversible growth arrest in mammalian cells. Identification of the mechanism of action of BrdU would provide a novel insight into the molecular mechanisms of cellular senescence. Here, we showed that a basic domain in the histone H2B N‐terminal tail, termed the HBR (histone H2B repression) domain, is involved in the action of BrdU. Notably, deletion of the HBR domain causes destabilized nucleosome positioning and derepression of gene expression, as does BrdU. We also showed that the genes up‐regulated by BrdU significantly overlapped with those by deletion of the HBR domain, the result of which suggested that BrdU and deletion of the HBR domain act in a similar way. Furthermore, we showed that decreased HBR domain function induced cellular senescence or facilitated the induction of cellular senescence. These findings indicated that the HBR domain is crucially involved in the action of BrdU, and also suggested that disordered nucleosome organization may be involved in the induction of cellular senescence.

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“…Yaşlanma için yapılan tanımlar bir organizma kadar tek bir hücre için de geçerlidirler. Hatta yıldızlar, galaksiler gibi cansız maddelerle, kültür gibi nicel olarak ölçülemeyen kavramlar için de yaşlanmadan söz edilebilmektedir 3 .…”

Section: Yaşlanmaunclassified

Yaşlanmayan Hücre

Kökbaş¹,

Tuli²

2014

Arşiv Kaynak Tarama Dergisi

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ÖZETYaşlanma ve ölümü engelleme konularındaki çalışmalar her zaman insanoğlunun odak noktalarından olmuştur ve bu uğurda birçok çalışmaya imza atmasına sebep olmuştur. Bütün bunların sonucunda, ilerleyen bilimsel gelişmeler çerçevesinde beklenen insan ömrü hem kadında hem de erkeklerde belirgin olarak uzamıştır. Bütün bunlara rağmen mevcut yaşam beklentisini yeterli görmeyen insanoğlu, yaşlanmanın ve bu sürecin sonunda meydana gelecek olan ölümün sebeplerini, fizyolojisini ve olabilecek alternatif tedavi biçimlerini anlamaya girişmiştir. Bu derlemede, gerek tarihi gerekse de güncel bilgilerimiz ışığında yaşlılığı önlemek yolunda Schizosaccharomyces pombe üzerine yapılan çalışmaların insan ömrüne yapacağı katkılar tartışılmaktadır. Anahtar kelimeler: Yaşlanma, yaşlanmama, ölüm, hücre ölümü, schizosaccharomyces pombe. ABSTRACTAll the studies, discussions and findings in order to prevent ageing or final death have to draw attention for a long time. Due to numerous studies related with ageing and its physiology, the expected life-span of human have to be significantly prolonged for both male and female species. However, due to the human nature that did not found the reached level sufficient, a great occupation realized to discover the physiology and alternative therapy strategies to quit the progression of ageing and the death. In this manuscript, the possible causes, theories and physiologic changes related with etiology of ageing which contribute to human life time with studies about Schizosaccharomyces pombe, have been discussed under old and recent literature.

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Changes in the higher order organization of DNA during aging of human fibroblast-like cells

Cited by 21 publications

References 31 publications

The effects of in vitro age and culture state on histone variant synthesis in human diploid fibroblasts

The effects of in vitro age and culture state on histone variant synthesis in human diploid fibroblasts

The key role of a basic domain of histone H2B N‐terminal tail in the action of 5‐bromodeoxyuridine to induce cellular senescence

Yaşlanmayan Hücre

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