2012
DOI: 10.1039/c2dt30166k
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Changes in the heme ligation during folding of a Geobacter sulfurreducens sensor GSU0935

Abstract: Ligand binding and substitution reactions are important for metalloprotein folding and function. The heme sensor of a methyl-accepting chemotaxis GSU0935 is a c-type cytochrome from the bacterium Geobacter sulfurreducens. The heme domain switches one of its axial ligands from H(2)O to a low-spin ligand, presumably Met, upon reduction. The study analyzes the stability and folding kinetics of the ferric domain. Guanidine hydrochloride denaturation yields the low-spin heme species arising from coordination of the… Show more

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Cited by 4 publications
(4 citation statements)
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“…Heme-based redox sensors with c -type (covalently attached: Figure E) heme have been reported. Importantly, autophosphorylation, perhaps as part of a two-component signal transduction system, is regulated by heme redox changes in MA4561, a heme-based sensor with a c -type heme . Among other heme-based sensors with c -type heme, including GSU582/GSU935, , DcrA has been extensively studied with respect to structural and physicochemical properties of the isolated heme domain . Unfortunately, catalytic and functional regulation of DcrA by heme redox changes has not been examined.…”
Section: Heme Redox Sensors With C-type and Non-c-type Heme Iron Comp...mentioning
confidence: 99%
“…Heme-based redox sensors with c -type (covalently attached: Figure E) heme have been reported. Importantly, autophosphorylation, perhaps as part of a two-component signal transduction system, is regulated by heme redox changes in MA4561, a heme-based sensor with a c -type heme . Among other heme-based sensors with c -type heme, including GSU582/GSU935, , DcrA has been extensively studied with respect to structural and physicochemical properties of the isolated heme domain . Unfortunately, catalytic and functional regulation of DcrA by heme redox changes has not been examined.…”
Section: Heme Redox Sensors With C-type and Non-c-type Heme Iron Comp...mentioning
confidence: 99%
“…15 In metalloprotions, the interconversion between alternate conformers can be driven by changes in metal ligation. 612 One of the most thoroughly studied ligand-mediated conformational changes for a metalloprotein is the alkaline conformational transition of mitochondrial cytochrome c (Cyt c ). 13,14 The alkaline conformational transition occurs at moderately alkaline pH and involves replacement of the Met80 ligand in the 6 th coordination site of the heme with either Lys73 or Lys79 in the heme crevice loop (residues 70–85, shown in red in Figure 1).…”
Section: Introductionmentioning
confidence: 99%
“…There has been considerable recent interest in characterizing alternate conformers of proteins because they are believed to be important for function. In metalloprotions, the interconversion between alternate conformers can be driven by changes in metal ligation. One of the most thoroughly studied ligand-mediated conformational changes for a metalloprotein is the alkaline conformational transition of mitochondrial cytochrome c (Cyt c ). , The alkaline conformational transition occurs at moderately alkaline pH and involves replacement of the Met80 ligand in the sixth coordination site of the heme with either Lys73 or Lys79 in the heme crevice loop (residues 70–85, shown in red in Figure ). , The Lys73- and Lys79-heme alkaline conformers are often assumed to be structurally and functionally equivalent. We apply conformationally gated electron-transfer (gated ET) methods to a K79H variant of yeast iso-1-cytochrome c , iso-1-Cyt c , to show that the mechanisms of formation of alkaline conformers with ligands from position 73 versus 79 differ.…”
Section: Introductionmentioning
confidence: 99%
“…A change in oxidation state and coordination of the bound heme is believed to result in a conformational change that activates or deactivates the catalytic domain. [77,78] Although the sensor domains of these proteins have been fairly well characterized in vitro the redox inputs that actuate the signaling cascade are unknown.…”
Section: Cellular Sensing Of the Electrode Potentialmentioning
confidence: 99%