Changes in the Growth and Biochemical Composition of Nannochloropsis sp. Cultures Using Light-Emitting Diodes

Abstract

The marine microalgae Nannochloropsis sp. was cultured under a metal halide lamp (MH) or light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; yellow, LY; green, LG; white, LW); changes in growth, total carotenoid, chlorophyll a, amino acid and fatty acid profiles were investigated. LB-exposed cultures exhibited the highest specific growth rate (SGR) (0.32), whereas LY-exposed cultures showed the lowest SGR (0.18). After cultivation for 9 days, the maximum dry cell weight (g/L) of LB-exposed cultures was significantly higher than that of those exposed to other light conditions (LB>MH>LW≧LG≧LR>LY). The essential amino acid (EAA, %) contents of cultures exposed to LG, LB, LR and MH were higher than those exposed to LY and LW (P<0.05). Eicosapentaenoic acid and n-3 highly unsaturated fatty acid levels were significantly higher in MH-exposed cultures compared to those exposed to LY (P<0.05), with no marked difference compared to those exposed to LB, LG, LR and LW (P>0.05). The total carotenoid content was highest in LR-exposed cultures (18.0 mg/L), whereas MH showed the lowest (11.8 mg/L; P<0.05). Chlorophyll a content was highest in cultures exposed to LR compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

Growth and Nutritional Composition of Eustigmatophyceae Monodus subterraneus and Nannochloropsis oceanica in Autotrophic and Mixotrophic Culture

“…Twenty-five mL of the sample was set with sodium dilution buffer (pH 2.2), and a portion of the sample was analyzed with the ninhydrin method using an amino acid analyzer (HSAAA, Hitachi L-8800, Japan). Conditions of the analysis were as follows: column size, 4 × 150 mm; absorbance wavelength, 570 nm and 440 nm; reagent flow rate, 0.25 mL/min; buffer flow rate, 0.45 mL/ min; reactor temperature, 120 o C; reactor size, 15 m; and analysis time, 65 min (Park et al 2013).…”

Adaptation of light emitting diode (LED) at culture on attachment plate of diatom

“…최근 미세조류의 효율적인 대량배양을 위해 모든 파장대를 구현할 수 있어 선호하는 특정 파장만을 공급해 줄 수 있는 light emitting diode (LED)가 적극 검토되고 있다 (Das et al, 2011). 실제 다양한 미세조류의 연구를 통 해 기존 배양방법 보다 양적으로나 질적 (영양조성)으 로 향상된 연구결과를 발표하고 있다 (Katsuda et al, 2006;Choi et al, 2013;Park et al, 2013;Saha et al, 2013;Markou, 2014) Fig. 1-B 9.0 ± 1.96 8.7 ± 0.44 8.6 ± 0.75 8.4 ± 1.00 10.2 ± 1.51 C14:1 3.0 ± 0.63 2.9 ± 0.17 2.8 ± 0.30 2.7 ± 0.39 3.4 ± 0.61 C16:1 3.2 ± 0.73 3.0 ± 0.12 3.2 ± 0.27 3.2 ± 0.43 3.6 ± 0.36 Sum of HUFA 3 7.5 ± 1.62 7.6 ± 0.42 7.4 ± 1.23 6.9 ± 0.96 8.6 ± 1.56 C18:2n6 4.7 ± 1.05 4.7 ± 0.26 4.7 ± 0.97 4.3 ± 0.59 5.4 ± 1.01 C20:5n3 2.8 ± 0.57 2.8 ± 0.17 2.7 ± 0.26 2.5 ± 0.37 3.2 ± 0.55 n-3 HUFA 2.8 ± 0.57 2.8 ± 0.17 2.7 ± 0.26 2.5 ± 0.37 3.2 ± 0.55 n-6 HUFA 4.7 ± 1.05 4.7 ± 0.26 4.7 ± 0.97 4.3 ± 0.59 5.4 ± 1.01 UI 32.…”