Changes in the Expression of Mitochondrial Morphology-Related Genes during the Differentiation of Murine Embryonic Stem Cells

Lee, Jeong Eon; Seo, Bong Jong; Han, Min Ji; Hong, Yean Ju; Hong, Kwonho; Song, Hyuk; Lee, Jeong-Woong; Tae, Jeong

doi:10.1155/2020/9369268

Cited by 14 publications

(10 citation statements)

References 50 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that there is an affirmative correlation between these proteins to their gene expressions (Lee et al . 2020). In the present study, upregulation of Mfn‐2/Drp‐1 was observed in most of the groups with LPS‐induction compared with the control.…”

Section: Discussionmentioning

confidence: 99%

Alterations of mitochondrial dynamics, inflammation and mineralization potential of lipopolysaccharide‐induced human dental pulp cells after exposure to N‐acetyl cysteine, Biodentine or ProRoot MTA

et al. 2021

View full text Add to dashboard Cite

Aim To investigate the effects of N‐acetyl cysteine (NAC), Biodentine, ProRoot MTA and their combinations, on cell viability, mitochondrial reactive oxygen species (mtROS) production, mineralization and on the expression of genes related to inflammatory cytokine production, mitochondrial dynamics and cell apoptosis of lipopolysaccharide (LPS)‐induced human dental pulp cells (hDPCs). Methodology Isolated hDPCs were exposed to 20 μg mL−1 of Escherichia coli (E. coli) LPS for 24 h, before the experiment, except for the control group. Eight experimental groups were assigned: (i) control (hDPCs cultured in regular medium), (ii) +LPS (hDPCs cultured in LPS medium throughout the experiment), (iii) −LPS/Media, (iv) −LPS/BD, (v) −LPS/MTA, (vi) −LPS/NAC, (vii) −LPS/BD + NAC and (viii) −LPS/MTA + NAC. Cell viability was measured using Alamar blue assay at 24 and 48 h. Production of mtROS was evaluated at 6 and 24 h by MitoSOX Red and MitoTracker Green. The expressions of IL‐6, TNF‐α, Bcl‐2, Bax, Mfn‐2 and Drp‐1 genes were investigated at 6 h using reverse transcriptase‐polymerase chain reaction (RT‐PCR). For differentiation potential, cells were cultured in the osteogenic differentiation media and stained using Alizarin red assay at 14 and 21 days. The Kruskal–Wallis test, Mann–Whitney U test and one‐way anova were performed for statistical analysis. Results NAC was associated with significantly greater LPS‐induced hDPC viability (P < 0.05). Both Biodentine and MTA extracts promoted cell survival, whereas the combination of NAC to these material extracts significantly increased the number of viable cells at 24 h (P < 0.05). Biodentine, MTA or NAC did not alter the mtROS level (P > 0.05). NAC supplementation to the MTA extract significantly reduced the level of IL‐6 and TNF‐α expression (P < 0.05). Regarding mitochondrial dynamics, the use of NAC alone promoted significant Mfn‐2/Drp‐1 expression (P < 0.05). Most of the groups exhibited a level of Bcl‐2/Bax gene expression similar to that of the control group. The increases in mineralization productions were observed in most of the groups, except the LPS group (P < 0.05). Conclusions The antioxidant effect of NAC was not evident under the LPS‐induced condition in DPC in vitro. NAC combined either with Biodentine or MTA improved LPS‐induced hDPCs survival at 24 h. The combination of NAC with MTA promoted mineralization.

show abstract

Section: Discussionmentioning

confidence: 99%

Alterations of mitochondrial dynamics, inflammation and mineralization potential of lipopolysaccharide‐induced human dental pulp cells after exposure to N‐acetyl cysteine, Biodentine or ProRoot MTA

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Mitochondria are highly dynamic organelles, moving around the cell through the microtubule network, constantly changing their morphology by fission and fusion processes which are tightly controlled to balance possible metabolic or environmental alterations [ 22 , 25 ]. Furthermore, mitochondria are very communicative with the other cellular organelles which manifest their importance for signaling cascades and normal cell function.…”

Section: Part 2: Emerging Evidence Of the Interplay Between Nrf2 And ...mentioning

confidence: 99%

NRF2 and Mitochondrial Function in Cancer and Cancer Stem Cells

Panieri

Pinho

Afonso

et al. 2022

Cells

View full text Add to dashboard Cite

The NRF2–KEAP1 system is a fundamental component of the cellular response that controls a great variety of transcriptional targets that are mainly involved in the regulation of redox homeostasis and multiple cytoprotective mechanisms that confer adaptation to the stress conditions. The pleiotropic response orchestrated by NRF2 is particularly relevant in the context of oncogenic activation, wherein this transcription factor acts as a key driver of tumor progression and cancer cells’ resistance to treatment. For this reason, NRF2 has emerged as a promising therapeutic target in cancer cells, stimulating extensive research aimed at the identification of natural, as well as chemical, NRF2 inhibitors. Excitingly, the influence of NRF2 on cancer cells’ biology extends far beyond its mere antioxidant function and rather encompasses a functional crosstalk with the mitochondrial network that can influence crucial aspects of mitochondrial homeostasis, including biogenesis, oxidative phosphorylation, metabolic reprogramming, and mitophagy. In the present review, we summarize the current knowledge of the reciprocal interrelation between NRF2 and mitochondria, with a focus on malignant tumors and cancer stem cells.

show abstract

“…In one mouse model, Fis1 levels could influence a pluripotent cell’s ability to differentiate through its influence on De novo fatty acid synthesis which required mitochondrial fission ( 148 ). However, minimal changes in Fis1 expression occur during mouse embryonic stem cell differentiation ( 149 ), suggesting further work needs to be done to elucidate the role of Fis1 during cellular differentiation.…”

Section: Current Perspective On Human Fis1 In Mitochondrial Dynamicsmentioning

confidence: 99%

Mitochondrial Fission Protein 1: Emerging Roles in Organellar Form and Function in Health and Disease

et al. 2021

View full text Add to dashboard Cite

Mitochondrial fission protein 1 (Fis1) was identified in yeast as being essential for mitochondrial division or fission and subsequently determined to mediate human mitochondrial and peroxisomal fission. Yet, its exact functions in humans, especially in regard to mitochondrial fission, remains an enigma as genetic deletion of Fis1 elongates mitochondria in some cell types, but not others. Fis1 has also been identified as an important component of apoptotic and mitophagic pathways suggesting the protein may have multiple, essential roles. This review presents current perspectives on the emerging functions of Fis1 and their implications in human health and diseases, with an emphasis on Fis1’s role in both endocrine and neurological disorders.

show abstract

Changes in the Expression of Mitochondrial Morphology-Related Genes during the Differentiation of Murine Embryonic Stem Cells

Cited by 14 publications

References 50 publications

Alterations of mitochondrial dynamics, inflammation and mineralization potential of lipopolysaccharide‐induced human dental pulp cells after exposure to N‐acetyl cysteine, Biodentine or ProRoot MTA

Alterations of mitochondrial dynamics, inflammation and mineralization potential of lipopolysaccharide‐induced human dental pulp cells after exposure to N‐acetyl cysteine, Biodentine or ProRoot MTA

NRF2 and Mitochondrial Function in Cancer and Cancer Stem Cells

Mitochondrial Fission Protein 1: Emerging Roles in Organellar Form and Function in Health and Disease

Contact Info

Product

Resources

About