Changes in the erythrocyte antioxidant enzyme system during transdermal estradiol therapy for secondary amenorrhea

Massafra, C; Buonocore, Giuseppe; Gioia, Dino; Sargentini, I

doi:10.3109/09513599609027982

Cited by 15 publications

(12 citation statements)

References 12 publications

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“…Oestrogens have been shown to possess remarkable antioxidant properties and numerous investigations have been carried out to elucidate the effects of female sex hormones on lipid peroxidation. [25][26][27][28] It has been demonstrated in many animal and human studies that oestrogens have potent antioxidant property and decrease lipid peroxidation levels including LDL oxidation. 27,[28][29][30] In this present study, while oestradiol levels in female players did not significantly change, in male players the levels increased by 28.1%.…”

Section: Discussionmentioning

confidence: 99%

Does underwater rugby stimulate the over-production of reactive oxygen species?

Çavaş

2004

Cell Biochem. Funct.

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Antioxidant enzymes in erythrocytes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and antioxidant sex hormone oestradiol in serum and malondialdehyde (MDA) production as a marker of lipid peroxidation in erythrocytes were investigated in male and female underwater rugby (UWR) players. Results showed that except for GSH-Px activity in female players, all antioxidant enzymes increased significantly (p < 0.05) after an UWR game. It was interesting to note that while the concentration of oestradiol in female players did not change, it increased significantly (p < 0.05) in male players. Lipid peroxidation (LPO) levels in female players as well as oestradiol concentration did not change significantly (p > 0.05) whereas LPO levels in male players increased significantly (p < 0.05) compared to pre-exercise values. In conclusion, the results showed that underwater rugby can stimulate over-production of ROS and antioxidant systems and affect oestradiol levels in male players. Because of increased LPO levels observed in male players, complex antioxidant supplementation including co-factors of antioxidant enzymes such as Cu, Zn, Fe, Se and antioxidant vitamins such as vitamin C and E may be recommended to players before the UWR game.

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Section: Discussionmentioning

confidence: 99%

Does underwater rugby stimulate the over-production of reactive oxygen species?

Çavaş

2004

Cell Biochem. Funct.

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“…Oestrogens have been reported to have signi®cant antioxidant potential as well, and various investigations have been carried out to elucidate the eects of female sex hormones on lipid peroxidation (Massafra et al 1996;Nakano et al 1987;Tranquilli et al 1995). Of these, several animal studies have shown that oestrogen inhibits lipid peroxidation in serum as well as tissue preparations (Nakano et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Exercise-induced oxidative stress and muscle performance in healthy women: role of vitamin E supplementation and endogenous oestradiol

Akova¹,

Sürmen-Gür

Gür³

et al. 2001

European Journal of Applied Physiology

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The purpose of this study was to investigate the individual and combined antioxidant effects of menstrual cycle phase-related alterations in blood serum oestradiol concentrations and of dietary vitamin E supplementation on exercise-induced oxidative stress and muscle performance. A group of 18 sedentary women, aged 19-35 years, were given supplements of 300 mg alpha-tocopherol (n = 10) or placebo (n = 8) daily during the course of two menstrual cycles. The subjects exercised the knee isokinetically to exhaustion after cycling submaximally at 50% maximal oxygen uptake during the menstrual and preovulatory phases of their menstrual cycles. Blood samples were taken before and after the exercise, to evaluate haematocrit, plasma lactic acid and malondialdehyde concentrations, erythrocyte antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and apolipoprotein B containing lipoprotein (non-high density lipoprotein, HDL, fraction) oxidation. Serum vitamin E, follicle stimulating hormone, luteinizing hormone and oestradiol concentrations were measured in pre-exercise blood samples. Neither vitamin E supplementation nor oestradiol concentrations influenced SOD and GPx activities or the susceptibility of the non-HDL fraction to oxidation while at rest. Plasma malondialdehyde concentration was unaffected by exercise, however significant reductions in erythrocyte SOD and GPx activities and increased susceptibility of the non-HDL fraction to oxidation were noted after exercise. Exercise-induced changes were reduced when oestradiol concentration was high in the preovulatory phase, independent of the serum vitamin E concentrations. In addition, both pre- (r = 0.58, P < 0.05) and post-exercise (r = 0.73, P < 0.001) GPx activities in placebo administered subjects were positively correlated with oestradiol concentrations. In conclusion, these findings suggest a better protective role of oestradiol against oxidative injury, compared to vitamin E. Exhausting muscle performance was, however, not influenced by vitamin E supplementation and/or cycle-phase related changes in oestradiol concentrations.

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“…As a result, GSH serves an important function in fighting oxidative damage and helping prevent heart diseases. Additionally, the GSH level is consistently increased following various types of treatments and in different situations, such as amenorrhea, post-menopause or treatments with oral contraceptive drugs (14)(15)(16)(17). In the present study, blood samples were collected from females who were not taking contraceptive drugs, between ages 22 and 51 years and have regular menstrual cycles.…”

Section: Discussionmentioning

confidence: 94%

Effects of estrogen on glutathione and catalase levels in human erythrocyte during menstrual cycle

et al. 2014

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Abstract. The present study evaluated the effects of physiological serum estrogen during the menstrual cycle on glutathione (GSH) and catalase activities. The sample included 43 healthy females between the ages of 22 and 51 years. The subjects were divided into two groups according to the stage of the menstrual cycle. Group A consisted of 16 samples extracted between days 10 and 20 from the first day of menstruation when estrogen levels were considered to be at their highest. Group B consisted of 27 samples extracted during other times of the estimated 30 days of menstruation. Data showed that the estrogen level in group A (184±106 pg/ml) was higher than that in group B (105±56 pg/ml) (P<0.01). The GSH and catalase levels in group A (4.4±2.3 µg/mg and 210±72 IU/mg, respectively) were also significantly higher compared to the levels in group B (3.2±1.8 µg/mg and 168±62 IU/mg, respectively) (P <0.05). Spearman's rank correlation showed that the expression of catalase in red blood cells significantly correlated with serum estrogen level but not with GSH. However, the changes in estrogen plasma levels, erythrocyte GSH level and catalase activity suggested that the consumption of GSH and catalase in erythrocyte during the menstrual cycle may be associated with the level of estrogen present in the bloodstream.

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Changes in the erythrocyte antioxidant enzyme system during transdermal estradiol therapy for secondary amenorrhea

Cited by 15 publications

References 12 publications

Does underwater rugby stimulate the over-production of reactive oxygen species?

Does underwater rugby stimulate the over-production of reactive oxygen species?

Exercise-induced oxidative stress and muscle performance in healthy women: role of vitamin E supplementation and endogenous oestradiol

Effects of estrogen on glutathione and catalase levels in human erythrocyte during menstrual cycle

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