Changes in the energy metabolism of cultured lens epithelial cells in comparison with the fresh lens

An Impediment to Glutathione Diffusion in Older Normal Human Lenses: a Possible Precondition for Nuclear Cataract

“…The lenses produced 14n4p2n8 µmol per 48-hr incubation per lens (n l 20), which is consistent with the literature (Piper et al, 1990 ;Wood and Truscott, 1993). No significant correlation was found in this study between lactate production and age.…”

Method for measuring a comprehensive energy budget in a proliferating cell system over multiple cell cycles

“…Moreover, the contribution to total ATP turnover by aerobic glycolysis was significant. This is in keeping with studies on platelets (Guppy et al, 1995), thymocytes (Guppy et al, 1993), lens epithelial cells (Piper et al, 1990), smooth muscle cells (Boehm et al, 1995), embryos (Dworkin and Dworkin-Rastl, 1991), cancer cells (Neermann and Wagner, 1996;Fitzpatrick et al, 1993), hair follicles (Kealey et al, 1994), lymphocytes (Roos and Loos, 1973), and assorted cells and cell lines (Lynch and Balaban, 1987;Nell et al, 1990;Lanks and Li, 1988). It appears that, in general, the oxidation of glucose is not a significant source of energy for cells.…”

Low-intensity ultraviolet A irradiation of the lens capsule to remove lens epithelial cells during cataract surgery

“…1 Lens epithelial cells (LECs) are germinal epithelial cells with high metabolic rates. 2,3 The primary function of these cells is to produce lens fibers throughout life. 1 When an IOL is implanted in the bag, the LECs produce well-known postoperative complications such as various types of anterior capsule opacification (ACO) and posterior capsule opacification (PCO).…”