Changes in the endoplasmic reticulum lipid properties in response to low temperature in Brassica napus

Tasseva, Guergana; Virville, Jacques Davy de; Cantrel, Catherine; Moreau, F.; Zachowski, Alain

doi:10.1016/j.plaphy.2004.10.001

Cited by 76 publications

(61 citation statements)

References 40 publications

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“…A aclimatação induz mudanças morfológicas, fisiológicas, bioquímicas e moleculares que tornam as plantas resistentes não somente a temperaturas congelantes, mas especialmente à desidratação provocada pelo congelamento (Rapacz, 1999;Hawkins et al, 2002;Gusta et al, 2004;Tasseva et al, 2004;Asghari et al, 2008;Stavang et al, 2008).…”

Section: Introductionunclassified

“…Na Região Sul do Brasil, é comum a ocorrência de geadas extemporâneas e consecutivas, por vários dias seguidos, bem como de diferentes intensidades, sem que, antecipadamente, tenham sido registradas temperaturas do ar com potencial de aclimatação (10 e 15ºC) (Rapacz, 1999;Rapacz et al, 2001;Rife & Zeinali, 2003;Tasseva et al, 2004). Os efeitos de intensidades e condições de ocorrência de geadas em canola, bem como do grau de resposta à aclimatação das plantas ao frio, não são conhecidos, especialmente no caso dos genótipos atualmente indicados para cultivo no Brasil.…”

Section: Introductionunclassified

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Aclimatação ao frio e dano por geada em canola

Dalmago

Cunha

Santi

et al. 2010

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a influência da aclimatação ao frio sobre o dano causado pela geada em diferentes estádios fenológicos de genótipos de canola. Foram realizados cinco experimentos em ambiente controlado, em 2006, 2007 e 2008. Os fatores avaliados foram: genótipos, aclimatação (com; sem), intensidades de geada, estádios de desenvolvimento de plantas, regimes de aclimatação e regimes de geada. As variáveis avaliadas foram: queima de folhas, massa de matéria seca, estatura de plantas, duração de subperíodo, componentes de rendimento e rendimento de grãos. A aclimatação ao frio, antes da geada, resultou em menor queima de folhas e maior massa de matéria seca, em comparação a plantas não aclimatadas. As geadas foram prejudiciais a partir de -6°C no início do ciclo de desenvolvimento, principalmente em plantas não aclimatadas, e a partir de -4ºC na floração, com redução do número de síliquas e do número de grãos por síliqua. A aclimatação após as geadas não contribuiu para a tolerância da canola a esse evento. Geadas consecutivas não acarretaram maior prejuízo à canola. A aclimatação de plantas de canola antes da geada reduz os danos, principalmente quando a geada ocorre no início do desenvolvimento das plantas.Termos para indexação: Brassica napus, estresse térmico, temperatura baixa, tolerância ao frio. Acclimatization to cold and frost-injury in canolaAbstract -The objective of this work was to evaluate the influence of cold acclimatization on frost damage at different phenological stages of canola genotypes. Five experiments were carried out under controlled conditions, in 2006, 2007, and 2008. The evaluated factors were: genotypes, acclimatization (with; without), frost gradient, plant developmental stages, acclimatization regimes and frost regimes. The evaluated variables were: leaf scorching symptoms, dry weight, plant height, length of subperiod, yield components and grain yield. The acclimatization before frost resulted in lesser leaf scorching symptoms and higher dry matter in comparison to plants not acclimated. Frosts were harmful from -6°C in early plant development stage, mainly in nonacclimated plants, and from -4ºC at flowering, reducing the number of pods and the number of grains per pod. Acclimatization after frost did not contribute to the tolerance of canola to frost. Consecutive frosts caused no greater impairment to canola. The canola acclimatization before frost reduces damage caused by it, mainly when the frost occur in the initial plant development stages.

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Section: Introductionunclassified

Aclimatação ao frio e dano por geada em canola

Dalmago

Cunha

Santi

et al. 2010

Pesq. agropec. bras.

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“…22 for review). Interestingly, some of these functions overlap with those proposed for alterations in cell membrane phospholipid composition that have been observed under cold or drought stress (23)(24)(25)(26). It is therefore not entirely clear whether GlyBet accumulation is a protective mechanism in itself, a symptom of stress susceptibility (27), or merely a consequence of increased phospholipid turnover (10,25).…”

mentioning

confidence: 95%

Biochemical Characterization of Two Wheat Phosphoethanolamine N-Methyltransferase Isoforms with Different Sensitivities to Inhibition by Phosphatidic Acid

Jost¹,

Berkowitz²,

Shaw

et al. 2009

Journal of Biological Chemistry

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In plants the triple methylation of phosphoethanolamine to phosphocholine catalyzed by phosphoethanolamine N-methyltransferase (PEAMT) is considered a rate-limiting step in the de novo synthesis of phosphatidylcholine. Besides being a major membrane phospholipid, phosphatidylcholine can be hydrolyzed into choline and phosphatidic acid. Phosphatidic acid is widely recognized as a second messenger in stress signaling, and choline can be oxidized within the chloroplast to yield the putative osmoprotectant glycine betaine. Here we describe the cloning and biochemical characterization of a second wheat PEAMT isoform that has a four times higher specific activity than the previously described WPEAMT/TaPEAMT1 enzyme and is less sensitive to product inhibition by S-adenosyl homocysteine, but more sensitive to inhibition by phosphocholine. Both enzymes follow a sequential random Bi Bi mechanism and show mixedtype product inhibition patterns with partial inhibition for TaPEAMT1 and a strong non-competitive component for TaPEAMT2. An induction of TaPEAMT protein expression and activity is observed after cold exposure, ahead of an increase in gene expression. Our results demonstrate direct repression of in vitro enzymatic activities by phosphatidic acid for both enzymes, with TaPEAMT1 being more sensitive than TaPEAMT2 in the physiological concentration range. Other lipid ligands identified in protein-lipid overlays are phosphoinositide mono-as well as some di-phosphates and cardiolipin. These results provide new insights into the complex regulatory circuits of phospholipid biosynthesis in plants and underline the importance of head group biosynthesis in adaptive stress responses. Phosphatidylcholine (PC),4 one of the major plasma membrane phospholipids in most eukaryotes, has been shown to undergo changes in turnover in response to various environmental stresses in plants (for review see Refs. 1, 2) and has a major impact on cell proliferation and apoptosis in mammals (for review see Ref.3).In single and multicellular organisms, biosynthetic routes leading to the production of PC are diverse and seem to depend on the availability of precursors for the synthesis of the hydrophilic head group, phosphocholine (P-Cho, see supplemental Fig. S1). Plants use the free phosphobase methylation pathway and can catalyze the conversion of serine to ethanolamine via a unique serine decarboxylase (4). Ethanolamine undergoes phosphorylation followed by the triple methylation of the free phosphobase to P-Cho, catalyzed by PEAMT (5, 6). P-Cho can then be used as the head group to form PC by the two enzymes P-Cho cytidylyltransferase and CDP-choline:diacylglycerol phosphotransferase, that are part of the Kennedy pathway in eukaryotes (7). Genetic evidence was recently presented in Plasmodium that the knockout mutant of the PfPMT gene is unable to synthesize PC in the absence of an external choline supply (8). Plasmodium therefore lacks the enzyme phosphatidylethanolamine methyltransferase found in fungi and mammals. Because higher plants lac...

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“…This concerns most importantly the desaturases FAD2, FAD3, and SSI2 in heat and the phosphatidic acid binding protein TGD2 in cold treatment. Finally, it is important to note that heat and cold do not trigger the membrane fluidity compensation mechanism during the first 6 h of the stress response, which has been observed 3 d after cold exposure of Arabidopsis rosettes (Welti et al, 2002) and Brassica napus cotyledons (Tasseva et al, 2004). This is an interesting observation considering that the dark-induced change is rapid, showing that plants potentially can modify the saturation level of their membrane lipids within a few hours.…”

Section: Parallel Changes Of Lipids and Transcripts Reflect The Repromentioning

confidence: 83%

“…One of the most well-known effects of prolonged chilling stress is increased desaturation of glycerolipids, serving as a compensation mechanism for decreased membrane fluidity (Williams et al, 1988;Welti et al, 2002;Tasseva et al, 2004). In agreement with this, coldtolerant species (Sakamoto et al, 2004;De Palma et al, 2008) and varieties (Horvath et al, 1983) and cold-acclimated plants (Degenkolbe et al, 2012) have been shown to have relatively high levels of desaturated glycerolipids.…”

Section: Introductionmentioning

confidence: 93%

Linking Gene Expression and Membrane Lipid Composition ofArabidopsis

et al. 2014

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Glycerolipid metabolism of plants responds dynamically to changes in light intensity and temperature, leading to the modification of membrane lipid composition to ensure optimal biochemical and physical properties in the new environment. Although multiple posttranscriptional regulatory mechanisms have been reported to be involved in the process, the contribution of transcriptional regulation remains largely unknown. Here, we present an integrative analysis of transcriptomic and lipidomic data, revealing large-scale coordination between gene expression and changes in glycerolipid levels during the Arabidopsis thaliana response to light and temperature stimuli. Using a multivariate regression technique called O2PLS, we show that the gene expression response is strictly coordinated at the biochemical pathway level and occurs in parallel with changes of specific glycerolipid pools. Five interesting candidate genes were chosen for further analysis from a larger set of candidates identified based on their close association with various groups of glycerolipids. Lipidomic analysis of knockout mutant lines of these five genes showed a significant relationship between the coordination of transcripts and glycerolipid levels in a changing environment and the effects of single gene perturbations.

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Changes in the endoplasmic reticulum lipid properties in response to low temperature in Brassica napus

Cited by 76 publications

References 40 publications

Aclimatação ao frio e dano por geada em canola

Aclimatação ao frio e dano por geada em canola

Biochemical Characterization of Two Wheat Phosphoethanolamine N-Methyltransferase Isoforms with Different Sensitivities to Inhibition by Phosphatidic Acid

Linking Gene Expression and Membrane Lipid Composition ofArabidopsis

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