Changes in the distribution of vinculin during preimplantation mouse development

Lehtonen, Eero; Reima, Ilkka

doi:10.1111/j.1432-0436.1986.tb00564.x

Cited by 42 publications

(16 citation statements)

References 36 publications

(23 reference statements)

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“…The presence of a-actinin in sea urchin eggs confirms the results obtained by Mabuchi et al (1985). Talin (our results) and vinculin (Lehtonen and Reima, 1986) were also detected in oocytes of hamster and mouse, respectively. The conservation of all these proteins among species, from invertebrates to higher vertebrate organisms, suggests that integrins may mediate, through cytoskeletal proteins, a highly conserved function such as fertilization.…”

Section: Recent Resultssupporting

confidence: 94%

Role of integrins and polyphosphoinositide metabolism during fertilization in sea urchin egg and hamster oocyte

Ciapa¹,

Nadaï²

1996

Invertebrate Reproduction & Development

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In almost all species studied to date, a transient increase in the intracellular fiee calcium concentration (Cq) occurs after fertilization and is essential for egg activation. How the sperm triggers this calcium signal remains, however, to be determined. In this brief review, we compare the mechanisms that are common to mammalian and invertebrate systems. In the light of our own data, we discuss how integrins that have recently been proposed to mediate sperm-egg binding and fusion in mammals might trigger egg activation through cytoskeletal structures. We suggest a model, leading to the calcium signal and common to all species, where phosphorylation on tyrosine and phospholipase Cy (PLCy) are interconnected pathways stimulated by a multimolecular complex involving integrins.

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Section: Recent Resultssupporting

confidence: 94%

Role of integrins and polyphosphoinositide metabolism during fertilization in sea urchin egg and hamster oocyte

Ciapa¹,

Nadaï²

1996

Invertebrate Reproduction & Development

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“…The antiserum that these investigators used was raised to a 12-mer of the cytoplasmic tail that corresponds to the carboxyl terminal end of p1 and lacks at least a portion of the molecule postulated to be required for focal adhesion localization or talin binding (Hayashi et al, 1990;Marcantonio et al, 1990;Tapley et al, 1989). It is also interesting to note that the staining pattern observed for a, is similar to that of vinculin, which is another cytoskeletal component of focal adhesions and is diffusely distributed throughout the cytoplasm but also concentrated in a submembranous layer (Lehtonen and Reima, 1986).…”

Section: II Oementioning

confidence: 91%

Identification and localization of integrin subunits in oocytes and eggs of the mouse

Evans¹,

Schultz

Kopf³

1995

Molecular Reproduction Devel

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Results of a recent study have implicated egg integrins in sperm binding to the egg plasma membrane (Blobel et al., 1991: Nature 356:248-252). In this report, immunoprecipitation was used to identify, and confocal immunofluorescence microscopy was used to localize, several different integrin subunits in mouse eggs. Antibodies to alpha 2, alpha 5, alpha v, and beta 1 subunits, as well as antibodies to the fibronectin receptor (FNR; alpha 5 beta 1 and/or alpha 3 beta 1) and vitronectin receptor (VNR; alpha v beta 3 and/or alpha v beta 5), detect polypeptides of the appropriate molecular weights following immunoprecipitation. beta 1 is localized preferentially to either the microvillar or amicrovillar membrane/cortical regions of eggs, and these asymmetric localizations depend on the antibody used. Proteins recognized by anti-FNR antibodies are localized preferentially to the amicrovillar membrane/cortical region. Germinal vesicle-intact oocytes display a symmetric plasma membrane distribution using beta 1 and FNR antibodies, and the asymmetric distribution develops as a consequence of oocyte maturation and is clearly observed by metaphase I. In contrast to the membrane localization of these integrin subunits, alpha 2, alpha 5, and VNR are predominantly localized in the cytoplasm of both oocytes and eggs. In the oocyte, each of these integrin subunits is uniformly distributed throughout the cytoplasm. Oocyte maturation is associated with a redistribution of alpha 5 and VNR, leading to an asymmetric cytoplasmic distribution with an increased localization towards the spindle. alpha v, which is localized in the plasma membrane/cortex of both oocytes and eggs, does not show such a change during oocyte maturation. Results of these experiments are discussed in the context of a role for integrins in mediating sperm plasma membrane-egg plasma membrane interactions leading to egg activation.

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“…These processes are essential in developmental phenomena [Beloussov et al, 1994]. Consequently, the role of the cytoskeleton in developing embryos or embryonic tissues has been extensively studied employing cytochalasins [Lehtonen and Reima, 1986;Sutherland and Calarco-Gillam, 1983;Wessells et al, 1971], colchicine [Beebe et al, 1979], nocodazole [Beebe et al, 1979;Maro and Pickering, 1984], and other cytoskeleton-disrupting drugs. A compromised microfilament system causes cell movement to cease and cell organization to break down.…”

Section: Model Of Spheroid Self-assemblymentioning

confidence: 99%

The role of actin filaments and microtubules in hepatocyte spheroid self-assembly

Tzanakakis

Hansen

2001

Cell Motil. Cytoskeleton

106

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Changes in the distribution of vinculin during preimplantation mouse development

Cited by 42 publications

References 36 publications

Role of integrins and polyphosphoinositide metabolism during fertilization in sea urchin egg and hamster oocyte

Role of integrins and polyphosphoinositide metabolism during fertilization in sea urchin egg and hamster oocyte

Identification and localization of integrin subunits in oocytes and eggs of the mouse

The role of actin filaments and microtubules in hepatocyte spheroid self-assembly

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