Changes in the content of fatty acid desaturases gene transcripts for Arabidopsis plants under adaptation to hypothermia

Selivanov, A. A.; Popov, V. N.; Antipina, O. V.; Пчелкин, В. П.; Цыдендамбаев, В. Д.; Мошков, И. Е.

doi:10.1134/s1021443717030153

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…During the hardening period, control potato plants demonstrated a significant increase in the activity of ∆9-desaturases (SDR value increased from 0.45 to 0.65; Table 3). This fact agrees with the earlier published data, according to which the lowtemperature hardening of cold-resistant A. thaliana plants resulted in an induction of the ADS gene encoding ∆9-acyl-lipid desaturase [18,19]. According to our results and earlier published data, an increased activity of Δ9-desaturases is probably explained by the necessity to form the first double bond for the provision of the further desaturases with substrates.…”

Section: Resultssupporting

confidence: 93%

Changes in Fatty Acid Composition and Lipid Content Occurring in Potato Leaves during Cold Hardening: the Role of ∆12-Acyl-Lipid Desaturase

Naraikina

Пчелкин

Цыдендамбаев

et al. 2020

Russ J Plant Physiol

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The qualitative composition and changes in the total fatty acid (FA) content in leaves of wild-type potato (Solanum tuberosum L., cv. Desnitsa) plants and plants transformed with the desA gene of Δ12-acyllipid desaturase from Synechocystis sp. PCC 6803 have been studied under cold hardening conditions (6 days at 5°С). During cold adaptation, plants of both types increase their cold resistance, but transformed plants significantly exceed control ones in relation to this parameter. Following cold hardening, the content of total etherified FA in leaves of control plants increases by almost 25% due to the decrease in the content of saturated FA and almost 30% increase in the content of polyunsaturated FA (PUFA); the growth of the PUFA content is provided mainly by linoleic (C 18:2 ) and linolenic (C 18:3 ) acids. Nonhardened leaves of transformed plants are characterized by the same FA content (especially C 18:3 ) as the leaves of hardened control plants. Due to this fact, increase in the total FA content in hardened transformants is not so significant as in the control; at the same time, the content of linoleic (C 18:2 ) acid increases by 30% (the calculated activity index for ∆12-desaturases also slightly increased). The growth of a hexadecatrienic (С 16:3 ) acid content in transformed plants probably results from a low-temperature activation of potato ω3-desaturases. The obtained data allow one to suppose that the higher resistance of transformed plants compared to control ones is connected with a constitutively increased PUFA content and determines more efficient cold hardening of such plants.

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Section: Resultssupporting

confidence: 93%

Changes in Fatty Acid Composition and Lipid Content Occurring in Potato Leaves during Cold Hardening: the Role of ∆12-Acyl-Lipid Desaturase

Naraikina

Пчелкин

Цыдендамбаев

et al. 2020

Russ J Plant Physiol

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“…Studies based on model objects have led to a significant improvement in the understanding of the molecular basis of plant cold tolerance. Several hundreds of genes involved in plant response to cold stress have been identified, including genes encoding transcription factors, phosphatases and kinases [ 7 , 8 , 9 , 10 ]. As a rule, in natural conditions, temperature decrease is gradual, allowing the plant to restructure its metabolism to form resistance to hypothermia [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profile and Functional State of Oat Plants (Avena sativa L.) Sown under Low-Temperature Conditions in the Cryolithozone

Nokhsorov,

Protopopov,

Sleptsov

et al. 2024

Plants

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Oats are one of the most useful and widespread cereal crops in the world. In permafrost conditions (Central Yakutia), based on metabolic changes in late summer-sown oat plants (Avena sativa L.), the key processes involved in the cold acclimation of a valuable cereal species were identified. During the onset of low ambient temperatures, metabolites from leaf samples were profiled using gas chromatography with mass spectrometry (GC-MS) and were analyzed using principal component analysis (PCA). A total of 41 metabolites were identified in oat leaves. It was found that acclimation to suboptimal temperatures during the fall period leads to biochemical (accumulation of mono- and disaccharides and decrease in fatty acids and polyols) as well as physiological and biophysical changes (decrease in leaf PRI reflectance indices and chlorophyll a fluorescence). Therefore, the study contributes to a more holistic understanding of oat metabolism under low-temperature cryolithozone stress. It is believed that the analysis of changes in leaf reflection properties and JIP-test parameters of chlorophyll a fluorescence using leaf metabolomic profiling can be used in the selection of valuable varieties of cereal crops to obtain plant fodders with high nutrient contents under conditions of a sharply continental climate.

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Physiological and transcriptomic analysis of postharvest Jiashi melon at different storage temperatures

Ning

Tang

Chen

et al. 2022

Postharvest Biology and Technology

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Changes in the content of fatty acid desaturases gene transcripts for Arabidopsis plants under adaptation to hypothermia

Cited by 5 publications

References 19 publications

Changes in Fatty Acid Composition and Lipid Content Occurring in Potato Leaves during Cold Hardening: the Role of ∆12-Acyl-Lipid Desaturase

Changes in Fatty Acid Composition and Lipid Content Occurring in Potato Leaves during Cold Hardening: the Role of ∆12-Acyl-Lipid Desaturase

Metabolomic Profile and Functional State of Oat Plants (Avena sativa L.) Sown under Low-Temperature Conditions in the Cryolithozone

Physiological and transcriptomic analysis of postharvest Jiashi melon at different storage temperatures

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