Changes in the Concentration of Monocytic Chemotaxic Protein-1 in Patients with Unstable Angina Treated with Arixtra

Потехина, А В; Arefieva, T. I.; Красникова, Т. Л.; Проваторов, С И; Масенко, В. П.; Osyaeva, M. K.; Ноева, Е А

doi:10.1007/s10517-011-1215-1

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Use of LMWH at low dose minimizes, but does not eliminate, the risk of side effects [51]. The synthetic heparin-like pentasaccharide drug Arixtra has shown anti-clotting activity without heparin-induced thrombocytopenia [52], and it has also been shown to decrease chemokine concentration and disrupt chemokine binding in human plasma [53]. Arixtra contains the rare 3-O sulfation that has been shown to be critical for this anti-clotting activity [54].…”

Section: Resultsmentioning

confidence: 99%

Structural Evidence for the Tetrameric Assembly of Chemokine CCL11 and the Glycosaminoglycan Arixtra™

2013

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Understanding chemokine interactions with glycosaminoglycans (GAG) is critical as these interactions have been linked to a number of inflammatory medical conditions, such as arthritis and asthma. To better characterize in vivo protein function, comprehensive knowledge of multimeric species, formed by chemokines under native conditions, is necessary. Herein is the first report of a tetrameric assembly of the human chemokine CCL11, which was shown bound to the GAG Arixtra™. Isothermal titration calorimetry data indicated that CCL11 interacts with Arixtra, and ion mobility mass spectrometry (IM-MS) was used to identify ions corresponding to the CCL11 tetrameric species bound to Arixtra. Collisional cross sections (CCS) of the CCL11 tetramer-Arixtra noncovalent complex were compared to theoretical CCS values calculated using a preliminary structure of the complex deduced using X-ray crystallography. Experimental CCS values were in agreement with theoretical values, strengthening the IM-MS evidence for the formation of the noncovalent complex. Tandem mass spectrometry data of the complex indicated that the tetramer-GAG complex dissociates into a monomer and a trimer-GAG species, suggesting that two CC-like dimers are bridged by Arixtra. As development of chemokine inhibitors is of utmost importance to treatment of medical inflammatory conditions, these results provide vital insights into chemokine-GAG interactions.

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Section: Resultsmentioning

confidence: 99%

Structural Evidence for the Tetrameric Assembly of Chemokine CCL11 and the Glycosaminoglycan Arixtra™

2013

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Pharmacological effects and clinical applications of ultra low molecular weight heparins

Liu

Sheng-li

Sheng

et al. 2014

DD&T

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Heparin, one of the common anticoagulants, is clinically used to prevent and treat venous thromboembolism (VTE). Though it has been the drug of choice for many advanced medical and surgical procedures with a long history, the adverse events, such as bleeding, heparin-induced thrombocytopenia (HIT), allergic reactions, follow. Therefore, low molecular weight heparins (LMWHs) and ultra low molecular weight heparins (ULMWHs), with lower molecular weights, higher anti-FXa activity, longer half-life times and lower incidence of adverse events than unfractionated heparin (UFH), were researched and developed. Fondaparinux, a chemically synthesized ULMWH of pentasaccharide, has the same antithrombin III (AT-III)-binding sequence as found in UFH and LMWH. In addition, AVE5026 and RO-14, another two ULMWHs, are obtained by selective chemical depolymerization. In this paper, we review the preparation process, pharmacological effects and clinical applications of fondaparinux, AVE5026 and RO-14.

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Changes in the Concentration of Monocytic Chemotaxic Protein-1 in Patients with Unstable Angina Treated with Arixtra

Cited by 2 publications

References 13 publications

Structural Evidence for the Tetrameric Assembly of Chemokine CCL11 and the Glycosaminoglycan Arixtra™

Structural Evidence for the Tetrameric Assembly of Chemokine CCL11 and the Glycosaminoglycan Arixtra™

Pharmacological effects and clinical applications of ultra low molecular weight heparins

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