Changes in the concentration of avenanthramides in response to salinity stress in CBF transgenic oat

Oraby, Hesham F.; El‐Tohamy, Maha F.; Kamel, A.; Ramadan, Mohamed Fawzy

doi:10.1016/j.jcs.2017.06.010

Cited by 9 publications

(20 citation statements)

References 24 publications

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“…Avenanthramide accumulation in oat grain is affected by weather and geographic conditions under which the studied material is cultivated [109,[150][151][152][153]. Changes in the concentration of avenanthramides in response to salinity stress in CBF3 transgenic oat demonstrated that these compounds might have a potential role in enhancing abiotic stress tolerance in oats [154]. Havrlentova et al [155] suggested that oats with higher β-D-glucan content may have thicker and, therefore, more insulating cell walls, better adapted to heat stress conditions.…”

Section: Abiotic Stress Resistancementioning

confidence: 99%

Wheat, Barley, and Oat Breeding for Health Benefit Components in Grain

Лоскутов

Khlestkina

2021

Plants

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Cereal grains provide half of the calories consumed by humans. In addition, they contain important compounds beneficial for health. During the last years, a broad spectrum of new cereal grain-derived products for dietary purposes emerged on the global food market. Special breeding programs aimed at cultivars utilizable for these new products have been launched for both the main sources of staple foods (such as rice, wheat, and maize) and other cereal crops (oat, barley, sorghum, millet, etc.). The breeding paradigm has been switched from traditional grain quality indicators (for example, high breadmaking quality and protein content for common wheat or content of protein, lysine, and starch for barley and oat) to more specialized ones (high content of bioactive compounds, vitamins, dietary fibers, and oils, etc.). To enrich cereal grain with functional components while growing plants in contrast to the post-harvesting improvement of staple foods with natural and synthetic additives, the new breeding programs need a source of genes for the improvement of the content of health benefit components in grain. The current review aims to consider current trends and achievements in wheat, barley, and oat breeding for health-benefiting components. The sources of these valuable genes are plant genetic resources deposited in genebanks: landraces, rare crop species, or even wild relatives of cultivated plants. Traditional plant breeding approaches supplemented with marker-assisted selection and genetic editing, as well as high-throughput chemotyping techniques, are exploited to speed up the breeding for the desired genotуpes. Biochemical and genetic bases for the enrichment of the grain of modern cereal crop cultivars with micronutrients, oils, phenolics, and other compounds are discussed, and certain cases of contributions to special health-improving diets are summarized. Correlations between the content of certain bioactive compounds and the resistance to diseases or tolerance to certain abiotic stressors suggest that breeding programs aimed at raising the levels of health-benefiting components in cereal grain might at the same time match the task of developing cultivars adapted to unfavorable environmental conditions.

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Section: Abiotic Stress Resistancementioning

confidence: 99%

Wheat, Barley, and Oat Breeding for Health Benefit Components in Grain

Лоскутов

Khlestkina

2021

Plants

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“…The avenanthramide‐rich extract (AE) was obtained as reported by Oraby et al. (2017) with minor modifications. Samples (5 g) were extracted with 25 ml of 80% ethanol with 1% of glacial acetic acid at 50°C for 25 min using a magnetic stirrer.…”

Section: Methodsmentioning

confidence: 99%

Polyphenols and avenanthramides extracted from oat ( Avena sativa L.) grains and sprouts modulate genes involved in glucose and lipid metabolisms in 3T3 L1 adipocytes

et al. 2021

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“…However, over the years, quite a few reports attempted to semi-quantify one or more “minor” AVAs, mostly based on retention time and existing standards of 2p, 2f and 2c. Examples included semi-quantification of AVA 5p [ 12 ], 5p and 4p [ 16 ], 5p, 4p, 3f/4f, and 2dp [ 17 ], 2fd and 2pd [ 18 ], 2s, 1c, 1p, 1f, and 1s [ 19 ], 2fd, 2pd, 3p, 3f, and 5f [ 20 ], and 8 [ 21 ], 10 [ 22 ] or 22 [ 23 ] total distinguishable AVA peaks (The last three all included the common three).…”

Section: Introductionmentioning

confidence: 99%

Simplified Analysis and Expanded Profiles of Avenanthramides in Oat Grains

Woolman

Liu

2022

Foods

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Uniquely, oats contain avenanthramides (AVAs), a group of phenolic alkaloids, exhibiting many health benefits. AVA analysis involves extraction with alcohol-based solvents and HPLC separation with UV and/or mass spectrometer detectors. There are many reported methods to extract AVAs. Almost all entail multiple extractions. The whole procedure is time- and labor-intensive. Furthermore, most quantifications are limited to three common AVAs (2f, 2p, 2c). The present study compared three extraction methods (all at 50 °C) for their effects on AVA concentrations and composition (% relative to total AVA) of oat grains. These included triplicate extractions with 80% ethanol containing 10 mM phosphate buffer (pH 2.0) (A), triplicate extractions with 80% ethanol (B), and a single extraction with 80% ethanol (C), while keeping solid/total solvent ratio at 1/60 (g/mL) and total extraction time of 60 min. Results showed that 80% buffered ethanol gave significantly lower AVA contents than 80% ethanol, while single and triplicate extractions with 80% ethanol produced the same extractability. However, the extraction method had no effect on AVA composition. Using 0.25 g sample size instead of 0.5 g saved extractants by half, without affecting AVA measurements. Consequently, a simplified method of extraction was developed, featuring Method C. The present study also expanded profiling individual AVAs beyond AVA 2c, 2p and 2f. Other AVAs identified and semi-quantified included 5p, 4p, 3f/4f, and 2pd. The simplified analysis was validated by measuring 16 selected oat grain samples. Some of these grains had relatively high contents of 4p, 3f/4f and 2pd, which have been considered minor AVAs previously.

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Changes in the concentration of avenanthramides in response to salinity stress in CBF transgenic oat

Cited by 9 publications

References 24 publications

Wheat, Barley, and Oat Breeding for Health Benefit Components in Grain

Wheat, Barley, and Oat Breeding for Health Benefit Components in Grain

Polyphenols and avenanthramides extracted from oat ( Avena sativa L.) grains and sprouts modulate genes involved in glucose and lipid metabolisms in 3T3 L1 adipocytes

Simplified Analysis and Expanded Profiles of Avenanthramides in Oat Grains

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