Changes in the capillary bed of skeletal muscle at various times after nerve section

Chernukh, A. M.; Alekseeva, N. N.

doi:10.1007/bf00799875

Cited by 12 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In regard to the long-term disuse, the significant decrease in C/F ratio and N CAF with 8-wk TTX treatment (Figs. 4 and 5) is consistent with reports of greater capillary loss with increased duration of disuse (3,13). Because V RBC was no longer significantly elevated at 8 wk (Fig.…”

Section: Discussionsupporting

confidence: 88%

“…A similar modulation during EDL muscle disuse (peroneal nerve-crush model) was reported by Zaida and co-workers (29). Capillarity increased after a chronic administration of prazosin that is known to elevate blood flow (3,29). Although other as-yet-undiscovered factors [such as a differential susceptibility to sympathectomy (15)] could also contribute to the differential response in capillarity seen in EDL and Gas muscles, the present data are consistent with the proposed mechanism that links capillarity and flow (4).…”

Section: Discussionsupporting

confidence: 78%

See 1 more Smart Citation

Differential microvascular response to disuse in rat hindlimb skeletal muscles

Tyml

Mathieu-Costello

Cheng

et al. 1999

Journal of Applied Physiology

View full text Add to dashboard Cite

The aim of the study was to address discrepant findings in the literature regarding coupling between decreased functional demand during disuse and reduced capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc. Res. 49: 17-32, 1995] that severe disuse of rat extensor digitorum longus (EDL) muscle caused by a 2-wk application of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by capillary loss. Using the same animal model, the present study examined whether this absence of coupling could be explained in terms of 1) too short a duration of disuse and 2) muscle-specific response to disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were determined by morphometry in the EDL muscle (control, 2- and 8-wk groups) and in the superficial portion of medial gastrocnemius (Gas) muscle (control, 2 wk). In both muscles, microvascular blood flow was evaluated by intravital microscopy [red blood cell velocity in capillaries (V(RBC))] and by laser Doppler flowmetry (LDF). Regardless of duration of TTX application or muscle type, TTX-induced disuse resulted in a significant reduction of fiber area (44-71%). However, capillary density increased in EDL muscle (both at 2 and 8 wk) but not in Gas muscle. C/F ratio decreased in EDL muscle at 8 wk (18%) and in Gas muscle (39%). This indicates that the effect on capillarity depended on duration of disuse and on muscle type. V(RBC) and LDF signal were significantly larger in EDL than in Gas muscle. Analysis of change in capillarity vs. V(RBC) suggested that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling between functional demand and capillarity could be due to the presence/absence of flow-related angiogenesis superimposed on the capillary removal process during disuse.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 78%

Differential microvascular response to disuse in rat hindlimb skeletal muscles

Tyml

Mathieu-Costello

Cheng

et al. 1999

Journal of Applied Physiology

View full text Add to dashboard Cite

show abstract

“…The existing studies (Mrazkova and Puzanova 1971;Engel and Stonnington 1974;Chernukh and Alekseeva 1975;Large and Tyler 1985;Tyml et al 1999) are difficult to compare, because the denervation procedures were different and different parameters were measured.…”

Section: Introductionmentioning

confidence: 99%

Nerve injury affects the capillary supply in rat slow and fast muscles differently

et al. 2005

View full text Add to dashboard Cite

The goal of this study was to determine the acute effects of permanent denervation on the length density of the capillary network in rat slow soleus (SOL) and fast extensor digitorum longus (EDL) muscles and the effect of short-lasting reinnervation in slow muscle only. Denervation was performed by cutting the sciatic nerve. Both muscles were excised 2 weeks later. Reinnervation was studied 4 weeks after nerve crush in SOL muscle only. Capillaries and muscle fibres were visualised by triple immunofluorescent staining with antibodies against CD31 and laminin and with fluorescein-labelled Griffonia (Bandeira) simplicifolia lectin. A recently developed stereological approach allowing the estimation of the length of capillaries adjacent to each individual fibre (Lcap/Lfib) was employed. Three-dimensional virtual test grids were applied to stacks of optical images captured with a confocal microscope and their intersections with capillaries and muscle fibres were counted. Interrelationships among capillaries and muscle fibres were demonstrated with maximum intensity projection of the acquired stacks of optical images. The course of capillaries in EDL seemed to be parallel to the fibre axes, whereas in SOL, their preferential direction deviated from the fibre axes and formed more cross-connections among neighbouring capillaries. Lcap/Lfib was clearly reduced in denervated SOL but remained unchanged in EDL, although the muscle fibres significantly atrophied in both muscle types. When soleus muscle was reinnervated, capillary length per unit fibre length was completely restored. The physiological background for the different responses of the capillary network in slow and fast muscle is discussed.

show abstract

“…the gastrocnemius [Chernukh and Alekseeva, 1975;Tyml et al, 1999] and soleus muscles [Čebašek et al, 2006;Degens et al, 2008], show regression of capillaries already in the early denervation period. Tyml et al [1999] speculated that such a specific postponed decrease in EDL capillaries to early denervation could be a consequence of the specific high susceptibility of EDL muscles to sympathectomy, because they have observed an increased blood flow after tetrodotoxin denervation (that could induce angiogenesis or prevent rarefaction of capillaries) only in the EDL but not in gastrocnemius muscles.…”

Section: Muscle Fibre Morphometry and Capillary Characteristicsmentioning

confidence: 99%

“…A number of studies have examined the manifold effects of injury on skeletal muscles, but only few of them focused on the morphological changes in capillaries [Chernukh and Alekseeva, 1975;Carpenter and Karpati, 1982;Large and Tyler, 1985;Tyml et al, 1999;Borisov et al, 2000;Dedkov et al, 2002;Wagatsuma et al, 2005;ČebaŠek et al, 2006, 2007Janáček et al, 2009].…”

Section: Introductionmentioning

confidence: 99%

Changes in the Capillarity of the Rat Extensor Digitorum Longus Muscle 4 Weeks after Nerve Injury Studied by 2D Measurement Methods

Čebašek¹,

Ribarič

2016

Cells Tissues Organs

View full text Add to dashboard Cite

We have previously shown by 3D study that 2 weeks after nerve injury there was no change in the length of capillaries per muscle fibre length in rat extensor digitorum longus muscle (EDL). The primary goal of the present 2D study was to determine the capillarity of rat EDL 4 weeks after various modes of nerve injury. Additionally, we wished to calculate the same capillary/fibre parameters that were used in our 3D stereological study. EDL muscles derived from denervated (4 weeks after nerve injury), re-innervated (4 weeks after two successive nerve crushes) and age-matched controls from the beginning (CON-1) and the end (CON-2) of the experiment were analysed in two ways. Global indices of capillarity, such as capillary density (CD) and capillary/fibre (C/F) ratio, were determined by automatic analysis, local indices as the number (CAF) and the length of capillaries around individual muscle fibres (Lcap) in relation to muscle fibre size were estimated manually by tracing the muscle fibre outlines and the transversally and longitudinally cut segments of capillaries seen in 5-µm-thin muscle cross sections. Four weeks after both types of nerve injury, CD increased in comparison to the CON-2 group (p < 0.001) due to atrophied muscle fibres in denervated muscles and probably proliferation of capillaries in re-innervated ones. Higher C/F, CAF (both p < 0.001) and Lcap (p < 0.01) in re-innervated than denervated EDL confirmed this assumption. Calculated capillary/fibre parameters were comparable to our previous 3D study, which strengthens the practical value to the adapted 2D method used in this study.

show abstract

Changes in the capillary bed of skeletal muscle at various times after nerve section

Cited by 12 publications

References 5 publications

Differential microvascular response to disuse in rat hindlimb skeletal muscles

Differential microvascular response to disuse in rat hindlimb skeletal muscles

Nerve injury affects the capillary supply in rat slow and fast muscles differently

Changes in the Capillarity of the Rat Extensor Digitorum Longus Muscle 4 Weeks after Nerve Injury Studied by 2D Measurement Methods

Contact Info

Product

Resources

About