Changes in the biomass production and total soluble protein spectra of nitrate-fed and nitrogen-fixing soybeans subjected to gradual water stress

Abstract

The effect of the sources of nitrogen nutrition (nitrogen fixation or nitrate assimilation) and a gradual water stress on the relative water content, total fresh and dry biomass production, leaf growth, and changes in the total soluble protein spectra were studied. The plants were cultivated as soil cultures in a naturally illuminated greenhouse. Comparative studies were carried out with respect to well-watered, control plants. Nitrogen-fixing control and drought plants had relatively smaller root development but be�er relative water content and large leaf area on the last sampling day than nitrate-fed soybean plants. Water deficit effects on plant biomass at the end of the period studied (21 days) were independent on the nitrogen source. There was no qualitative difference in the total soluble protein spectra of nitratefed and nitrogen-fixing soybean leaves neither with the progress of development nor under drought conditions. But there was a difference in response to drought in termostable proteins of nitrate-fed and nitrogen-fixing plants. The quantity of termostable proteins in inoculated control plants was lower in some degree compared to uninoculated ones. In inoculated plants the water stress caused an increase in the amount of soluble termostable proteins.

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

“…Nitrogen is required by plants in greater quantities than any other mineral elements to support their growth and development (Kirova et al, 2005). Based on the results of present investigation, higher total soluble nitrogen content was observed in plantlets derived from irradiated seeds as compared to the control samples.…”

Chlorophyll and Protein Changes Observed Post Co-Cultivation of Explants with Agrobacterium Tumefaciens in Soybean (Glycine max L. Merr.)

“…The results also indicate that the extraction protocol has undoubtedly and reproducibly trapped a comprehensive repertoire of proteins without degradation or contamination, particularly, in LS and TGx cultivars as alluded by Xu et al (2006;Natarajan et al, 2006). However, the results indicate a possible influence on polypeptide expression due to the cocultivation of explants with A. tumefaciens which may have induced physiological stress-related or transgenic proteins, as reported by Kirova et al (2005). Generally, as observed in Fig.…”

Phenylurea-type Cytokinin ameliorates the performance of young pea plants under salt stress

“…However, no general opinion exists on the exact role of proline, whether it plays a protective role (is has high capability to efficiently detoxify free radicals, and also acts as osmoprotector) or the considerable changes in its concentration are merely a symptom of stress (Czarnocka & Karpinski, 2018). A large amount of data indicated a positive correlation between the increased proline content and the strength of the stress and the presence and extent of adverse changes in the physiological status of plants (Kirova et al, 2005;Kaur & Asthir, 2015). Our results align with data from literature since the highest proline levels were found in salt-stressed pea plants.…”