Changes in the adult vertebrate auditory sensory epithelium after trauma

Oesterle, Elizabeth C.

doi:10.1016/j.heares.2012.11.010

Cited by 29 publications

(22 citation statements)

References 132 publications

(177 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The remarkably mild effect of neonatal IBC/IPhC ablation on the adult cochlea was surprising, because a number of studies suggest that these cells play important roles in cochlear development (1,(2)(3)(4)(5)(6). Therefore, we thought it was necessary to test whether IBC/IPhC ablation at later stages had a different effect.…”

Section: Ibc/iphc Ablation After the Onset Of Hearing Results In Profmentioning

confidence: 99%

“…S1A). IBCs and IPhCs, together with other SCs, are known to play critical roles during the development and maturation of the organ of Corti, in processes such as patterning of the epithelium, synaptogenesis, and initiation of electrical activity in auditory nerves before the onset of hearing and formation of extracellular matrices (1)(2)(3)(4)(5)(6)(7). SCs also are essential for the function of the mature organ of Corti, where they contribute to the maintenance of the reticular lamina at the apical surface of the epithelium (8), control the extracellular concentration of ions (e.g., K + ) (9, 10) and neurotransmitters (e.g., glutamate) (11), and support hair cell (HC) and auditory sensory neuron survival (5,(12)(13)(14)(15).…”

mentioning

confidence: 99%

“…Additionally, nonmammalian vertebrates regenerate HCs and SCs after damage and recover hearing, with the SCs being the source of the regenerative response (21)(22)(23). Indeed, if SCs are damaged by insults, the regenerative response is severely compromised (1,24). Thus, it is assumed that the presence of these cells in the postnatal cochlea is essential for hearing, but specific roles of IBCs and IPhCs in HC maintenance and cochlear function have not been established.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Spontaneous regeneration of cochlear supporting cells after neonatal ablation ensures hearing in the adult mouse

Lagarde

Wan

Zhang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Supporting cells in the cochlea play critical roles in the development, maintenance, and function of sensory hair cells and auditory neurons. Although the loss of hair cells or auditory neurons results in sensorineural hearing loss, the consequence of supporting cell loss on auditory function is largely unknown. In this study, we specifically ablated inner border cells (IBCs) and inner phalangeal cells (IPhCs), the two types of supporting cells surrounding inner hair cells (IHCs) in mice in vivo. We demonstrate that the organ of Corti has the intrinsic capacity to replenish IBCs/IPhCs effectively during early postnatal development. Repopulation depends on the presence of hair cells and cells within the greater epithelial ridge and is independent of cell proliferation. This plastic response in the neonatal cochlea preserves neuronal survival, afferent innervation, and hearing sensitivity in adult mice. In contrast, the capacity for IBC/IPhC regeneration is lost in the mature organ of Corti, and consequently IHC survival and hearing sensitivity are impaired significantly, demonstrating that there is a critical period for the regeneration of cochlear supporting cells. Our findings indicate that the quiescent neonatal organ of Corti can replenish specific supporting cells completely after loss in vivo to guarantee mature hearing function.deafness | cell ablation | hair cell | organ of Corti | glia

show abstract

Section: Ibc/iphc Ablation After the Onset Of Hearing Results In Profmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Spontaneous regeneration of cochlear supporting cells after neonatal ablation ensures hearing in the adult mouse

Lagarde

Wan

Zhang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Of the different types of supporting cells, Deiters' cells (DCs) have a pivotal role in repair after hair cell loss. Prompt sealing of the epithelial surface by DCs prevents expansion of damage by limiting the entry of the potassium-rich endolymph into the organ (McDowell et al 1989;Monzack and Cunningham 2013;Oesterle 2013).…”

Section: Introductionmentioning

confidence: 99%

How to Bury the Dead: Elimination of Apoptotic Hair Cells from the Hearing Organ of the Mouse

Anttonen

Belevich

Kirjavainen

et al. 2014

JARO

View full text Add to dashboard Cite

Hair cell death is a major cause of hearing impairment. Preservation of surface barrier upon hair cell loss is critical to prevent leakage of potassium-rich endolymph into the organ of Corti and to prevent expansion of cellular damage. Understanding of wound healing in this cytoarchitecturally complex organ requires ultrastructural 3D visualization. Powered by the serial block-face scanning electron microscopy, we penetrate into the cell biological mechanisms in the acute response of outer hair cells and glial-like Deiters' cells to ototoxic trauma in vivo. We show that Deiters' cells function as phagocytes. Upon trauma, their phalangeal processes swell and the resulting close cellular contacts allow engulfment of apoptotic cell debris. Apical domains of dying hair cells are eliminated from the inner ear sensory epithelia, an event thought to depend on supporting cells' actomyosin contractile activity. We show that in the case of apoptotic outer hair cells of the organ of Corti, elimination of their apices is preceded by strong cell body shrinkage, emphasizing the role of the dying cell itself in the cleavage. Our data reveal that the resealing of epithelial surface by junctional extensions of Deiters' cells is dynamically reinforced by newly polymerized F-actin belts. By analyzing Cdc42-inactivated Deiters' cells with defects in actin dynamics and surface closure, we show that compromised barrier integrity shifts hair cell death from apoptosis to necrosis and leads to expanded hair cell and nerve fiber damage. Our results have implications concerning therapeutic protective and regenerative interventions, because both interventions should maintain barrier integrity.

show abstract

“…Many types of insults, including acoustic trauma from loud noise and ototoxic drugs can damage hair cells resulting in hearing loss. Following this trauma, epithelial reorganization occurs in the cochlear whereby specialized columnar epithelial cells are replaced by an unspecialized flattened epithelium (Oesterle 2013). Ladrech et al (2017) have now used a model of amikacin (an aminoglycoside antibiotic) induced cochlear remodeling following hair cell loss to study these processes using immunohistochemistry.…”

Section: Epithelial Changes In Amikacin-damaged Organ Of Cortimentioning

confidence: 99%