Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding

Jian, Tian; Huang, Wen; Zeng, Lingbing; Jiang, Ming; Wu, Fan; Liu, Wei; Yang, Changgeng

doi:10.1016/j.aquaculture.2013.01.032

Cited by 77 publications

(57 citation statements)

References 52 publications

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“…Tilapia (O. niloticus), sturgeon (Acipenser naccarii), and trout (O. mykiss) experience nearly a 70% decrease in plasma triglyceride levels by 28, 40, and 40 days of fasting, respectively, with levels restored following 3 to 4 weeks of refeeding (Fig. 10B) (195,549). Subantarctic fur seal pups experienced an immediate 75% drop in plasma triglycerides through Phase I that remained low throughout Phase II (567).…”

Section: Triglyceridesmentioning

confidence: 93%

“…Liver activity and mRNA expression of LPL and HSL increase by 2-to 3-fold within 2 to 3 weeks of fasting for the Nile tilapia (O. niloticus) (549). Beta-oxidation of NEFA generates acetyl-CoA that is shuttled into the TCA cycle, or to a lesser extent, is used to produce ketone bodies (see below).…”

Section: Lipidsmentioning

confidence: 98%

“…The targeting of liver genes in the Nile tilapia using RT-PCR revealed the decline with fasting (up to 28 days) in the expression of fatty acid synthetase and HSL, and the increase expression of LPL, all of which were reversed with refeeding (549). The molecular basis of the capacity of elephant seal pups to maintain elevated levels of thyroid hormone during postweaning fasting was examined by Martinez et al (352).…”

Section: Gut Microbes In Fasting and Refeedingmentioning

confidence: 98%

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Integrative Physiology of Fasting

Secor

Carey

2016

Comprehensive Physiology

138

144

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Extended bouts of fasting are ingrained in the ecology of many organisms, characterizing aspects of reproduction, development, hibernation, estivation, migration, and infrequent feeding habits. The challenge of long fasting episodes is the need to maintain physiological homeostasis while relying solely on endogenous resources. To meet that challenge, animals utilize an integrated repertoire of behavioral, physiological, and biochemical responses that reduce metabolic rates, maintain tissue structure and function, and thus enhance survival. We have synthesized in this review the integrative physiological, morphological, and biochemical responses, and their stages, that characterize natural fasting bouts. Underlying the capacity to survive extended fasts are behaviors and mechanisms that reduce metabolic expenditure and shift the dependency to lipid utilization. Hormonal regulation and immune capacity are altered by fasting; hormones that trigger digestion, elevate metabolism, and support immune performance become depressed, whereas hormones that enhance the utilization of endogenous substrates are elevated. The negative energy budget that accompanies fasting leads to the loss of body mass as fat stores are depleted and tissues undergo atrophy (i.e., loss of mass). Absolute rates of body mass loss scale allometrically among vertebrates. Tissues and organs vary in the degree of atrophy and downregulation of function, depending on the degree to which they are used during the fast. Fasting affects the population dynamics and activities of the gut microbiota, an interplay that impacts the host's fasting biology. Fasting-induced gene expression programs underlie the broad spectrum of integrated physiological mechanisms responsible for an animal's ability to survive long episodes of natural fasting.

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Section: Triglyceridesmentioning

confidence: 93%

Section: Lipidsmentioning

confidence: 98%

Section: Gut Microbes In Fasting and Refeedingmentioning

confidence: 98%

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Integrative Physiology of Fasting

Secor

Carey

2016

Comprehensive Physiology

138

144

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“…Fasting also regulated the enzyme activities (Furné, García‐Gallego, Carmen Hidalgo, Morales, Domezain, Domezain & Sanz ; Abolfathi, Hajimoradloo, Ghorbani & Zamani ) and gene expressions in different tissues of fish (Han et al . ; Tian, Wen, Zeng, Jiang, Wu, Liu & Yang ). However, the mechanism of lipid metabolism has not yet fully elucidated in large yellow croaker when faced with fasting.…”

Section: Introductionmentioning

confidence: 99%

Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker,Larimichthys crocea(Richardson), during fasting

et al. 2016

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Over‐winter fasting and man‐made food deprivation to increase meat quality are common in the process of large yellow croaker (Larimichthys crocea) aquaculture. This study aimed to determine the changes in lipoprotein lipase (LPL) and fatty acid synthetase (FAS) activities and mRNA expressions, and the relationships between these changes and fat content in large yellow croaker liver and muscle tissues during fasting. A total of 2933 bp LPL cDNA, including an open reading frame of 1533 bp encoding 510 amino acids, and a 1233 bp fragment of FAS cDNA coding 411 amino acids were cloned. Expressions of both genes were ubiquitous. During a 35‐day fasting period, the hepatosomatic index and fat content in muscle and liver were significantly decreased (P < 0.05). mRNA levels of LPL increased significantly during the fasting period except the first 3 days, and FAS mRNA levels decreased significantly in both muscle and liver (P < 0.05) although there were some fluctuations. Muscle and liver LPL activities were significantly higher following fasting for 7 days, decreased to the initial value following fasting for 14 days, and elevating significantly afterwards (P < 0.05). FAS activities in muscle and liver maintained a significantly decreasing trend during the short‐term fasting (P < 0.05) and kept obviously rising thereafter (P < 0.05). Activities and mRNA levels of both LPL and FAS were not always consistent, which implied that both pre‐translational and post‐translational regulations existed during fasting. Our results suggest that the reasonable fasting time is 21 days before harvesting when fat content decreased significantly.

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“…In these experiments, the increase in LPL production also occurred with parallel rises in LPL gene expression, suggesting that adropin can not only induce LPL release but also trigger LPL synthesis via up-regulation of LPL gene expression. Previous studies pointed to the effect of LPL on lipid metabolism in tilapia (Oreochromis niloticus) in which hepatic LPL gene expression levels were synchronously increased after fasting (Han et al 2011, Tian et al 2013, and further, there was a negative correlation between TG levels and LPL levels (Tian et al 2013), suggesting LPL is an important lipolytic factor in tilapia. In mice, hepatic adropin gene expression is regulated by liver X receptor (LXR)-a (Kumar et al 2008), which is a nuclear receptor involved in cholesterol and triglyceride metabolism (Kalaany & Mangelsdorf 2006).…”

Section: Discussionmentioning

confidence: 99%

Adropin induction of lipoprotein lipase expression in tilapia hepatocytes

Lian

Liu

et al. 2016

Journal of Molecular Endocrinology

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The peptide hormone adropin plays a role in energy homeostasis. However, biological actions of adropin in non-mammalian species are still lacking. Using tilapia as a model, we examined the role of adropin in lipoprotein lipase (LPL) regulation in hepatocytes. To this end, the structural identity of tilapia adropin was established by 5 0 /3 0 -rapid amplification of cDNA ends (RACE). The transcripts of tilapia adropin were ubiquitously expressed in various tissues with the highest levels in the liver and hypothalamus. The prolonged fasting could elevate tilapia hepatic adropin gene expression, whereas no effect of fasting was observed on hypothalamic adropin gene levels. In primary cultures of tilapia hepatocytes, synthetic adropin was effective in stimulating LPL release, cellular LPL content, and total LPL production. The increase in LPL production also occurred with parallel rises in LPL gene levels. In parallel experiments, adropin could elevate cAMP production and up-regulate protein kinase A (PKA) and PKC activities. Using a pharmacological approach, cAMP/PKA and PLC/inositol trisphosphate (IP3)/PKC cascades were shown to be involved in adropin-stimulated LPL gene expression. Parallel inhibition of p38MAPK and Erk1/2, however, were not effective in these regards. Our findings provide, for the first time, evidence that adropin could stimulate LPL gene expression via direct actions in tilapia hepatocytes through the activation of multiple signaling mechanisms.

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Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding

Cited by 77 publications

References 52 publications

Integrative Physiology of Fasting

Integrative Physiology of Fasting

Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker,Larimichthys crocea(Richardson), during fasting

Adropin induction of lipoprotein lipase expression in tilapia hepatocytes

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