Changes in the abscisic acid levels and related gene expression during fruit development and ripening in bilberry (Vaccinium myrtillus L.)

Karppinen, Katja; Hirvelä, Elina; Nevala, Tiina; Sipari, Nina; Suokas, Marko; Jaakola, Laura

doi:10.1016/j.phytochem.2013.06.023

Cited by 71 publications

(54 citation statements)

References 38 publications

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“…Our previous studies have demonstrated an increase in ABA content and ABA biosynthesis at the onset of bilberry fruit ripening preceding anthocyanin accumulation (Karppinen et al, 2013, 2016). Similarly, Zifkin et al (2012) demonstrated a substantial increase in ABA level in highbush blueberries ( V. corymbosum ) at the initiation of fruit ripening suggesting a role for ABA in fruit ripening regulation.…”

Section: Discussionmentioning

confidence: 98%

“…The plants were placed in boxes (50 cm × 70 cm) with forest peat soil and watered well. The five developmental stages of bilberry fruit were collected as described earlier (Karppinen et al, 2013). …”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, earlier studies have given contradictory results on the role of ABA in the anthocyanin accumulation in other Vaccinium species (Percival and MacKenzie, 2007; Forney et al, 2009; Buran et al, 2012; Oh et al, 2018). In our previous study, an increase in endogenous ABA level accompanied by an increase in the expression of VmNCED1 , the key gene in ABA biosynthesis, was demonstrated at the onset of bilberry fruit ripening suggesting a role for ABA in bilberry ripening regulation (Karppinen et al, 2013). …”

Section: Introductionmentioning

confidence: 95%

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Abscisic Acid Regulates Anthocyanin Biosynthesis and Gene Expression Associated With Cell Wall Modification in Ripening Bilberry (Vaccinium myrtillus L.) Fruits

et al. 2018

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Ripening of non-climacteric bilberry (Vaccinium myrtillus L.) fruit is characterized by a high accumulation of health-beneficial anthocyanins. Plant hormone abscisic acid (ABA) and sucrose have been shown to be among the central signaling molecules coordinating non-climacteric fruit ripening and anthocyanin accumulation in some fruits such as strawberry. Our earlier studies have demonstrated an elevation in endogenous ABA level in bilberry fruit at the onset of ripening indicating a role for ABA in the regulation of bilberry fruit ripening. In the present study, we show that the treatment of unripe green bilberry fruits with exogenous ABA significantly promotes anthocyanin biosynthesis and accumulation both in fruits attached and detached to the plant. In addition, ABA biosynthesis inhibitor, fluridone, delayed anthocyanin accumulation in bilberries. Exogenous ABA also induced the expression of several genes involved in cell wall modification in ripening bilberry fruits. Furthermore, silencing of VmNCED1, the key gene in ABA biosynthesis, was accompanied by the down-regulation in the expression of key anthocyanin biosynthetic genes. In contrast, the treatment of unripe green bilberry fruits with exogenous sucrose or glucose did not lead to an enhancement in the anthocyanin accumulation neither in fruits attached to plant nor in post-harvest fruits. Moreover, sugars failed to induce the expression of genes associated in anthocyanin biosynthesis or ABA biosynthesis while could elevate expression of some genes associated with cell wall modification in post-harvest bilberry fruits. Our results demonstrate that ABA plays a major role in the regulation of ripening-related processes such as anthocyanin biosynthesis and cell wall modification in bilberry fruit, whereas sugars seem to have minor regulatory roles in the processes. The results indicate that the regulation of bilberry fruit ripening differs from strawberry that is currently considered as a model of non-climacteric fruit ripening. In this study, we also identified transcription factors, which expression was enhanced by ABA, as potential regulators of ABA-mediated bilberry fruit ripening processes.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Abscisic Acid Regulates Anthocyanin Biosynthesis and Gene Expression Associated With Cell Wall Modification in Ripening Bilberry (Vaccinium myrtillus L.) Fruits

et al. 2018

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“…It is possible that ABA regulates the maturation involving anthocyanin biosynthesis in non-climateric fruit such as the bilberry (Vaccinium myrtillus L.), blueberries (V. corymbosum), sweet cherries (Prunus avium L.) and grapes (Wheeler et al 2009;Ren et al 2010;Zifkin et al 2012;Karppinen et al 2013). In grapes, endogenous ABA concentrations increase sharply at veraison when the berries accumulate anthocyanin, and then gradually decrease toward harvest (Wheeler et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Effects of light emitting diode irradiation at night on abscisic acid metabolism and anthocyanin synthesis in grapes in different growing seasons

et al. 2015

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The effects of red-and blue-light irradiation at night on abscisic acid (ABA) synthesis and anthocyanin and sugar concentrations were examined in grape vines, which were grown in two different seasons. In grapes cultivated with early heating, the ABA concentrations were highest in blue-light-emitting diode (LED)-treated skin; however, those in grapes cultivated in the ordinary growing season were highest in red-LED-treated skin. The expressions of 9-cis-epoxycarotenoid dioxygenase (VvNCED1) and ABA 8 0 -hydroxylase (VvCYP707A1) were high in each treatment at veraison regardless of the growing season. In both seasons, anthocyanin concentrations were highest under the blue-LED treatment, followed by the red-LED treatment. The expressions of VlMYBA1-2, VlMYBA2, and VvUFGT coincided with anthocyanin concentrations. Sugar concentrations were increased by the blue-or red-LED treatment dependent on the growing season. The results suggest that blue-or red-LED irradiation at night may influence the ABA and anthocyanin metabolism including VvNCED1, VlMYBA1-2, and VlMYBA2 and sugar synthesis in grape berries, although the degree of the effects differs with the growing season.

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“…Much higher ABA concentrations were found in some other fruit species. For example, in bilberry (Vaccinium myrtillus) fruits, the ABA content may reach over 30,000 ng g −1 DW, which is more than 70 times higher than that in leaves (Karppinen et al 2013). Furthermore, the ABA concentration in mangosteens (Garcinia mangostana L.) might be as high as 6,600 ng g −1 DW in some developmental stages (Kondo et al 2002).…”

Section: Aba Content In Relation To Aba Biosynthesis and Catabolismmentioning

confidence: 99%

ABA Transport and Distribution in Relation to Its Function in Plants

Jia

2014

Abscisic Acid: Metabolism, Transport and Signaling

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Abscisic acid (ABA) is a phytohormone with key roles in various physiological processes, including germination, stomatal movement, and biotic and abiotic stress responses. Given that the sites of ABA biosynthesis and action are separated in plant cells, ABA transport is an important step in ABA signaling. ABA exhibits distinct patterns of compartmentalization, and its level in a specific compartment is not only determined by its rate of biosynthesis and catabolism, but also by the rates of ABA export and import. Compartmentalization ensures that the physiology of the plant is not affected by ABA under normal conditions and that redistribution of ABA in response to stress stimuli triggers ABA stress signaling. ABA is transported between cells or tissues and also systemically in whole plants. The expression of genes encoding key enzymes in the ABA biosynthesis pathway in vascular tissues and the overlap in the expression patterns of genes encoding enzymes involved in ABA biosynthesis and ABA transport implies the importance of ABA transport, particularly ABA systemic transport, in ABA functioning. The root-to-shoot transport of ABA further shows that ABA transport has critical roles in plant systemic signaling, and the molecular identification and functional characterization of ABA transporters provided direct evidence that ABA transport is required for ABA signaling. The ion-trap mechanism by pH and ABA transporters may operate synergistically, but in different manners, to control ABA transport and distribution. Further investigations on ABA transport and localization will contribute to our understanding of mechanisms that modulate ABA signaling in plants.

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Changes in the abscisic acid levels and related gene expression during fruit development and ripening in bilberry (Vaccinium myrtillus L.)

Cited by 71 publications

References 38 publications

Abscisic Acid Regulates Anthocyanin Biosynthesis and Gene Expression Associated With Cell Wall Modification in Ripening Bilberry (Vaccinium myrtillus L.) Fruits

Abscisic Acid Regulates Anthocyanin Biosynthesis and Gene Expression Associated With Cell Wall Modification in Ripening Bilberry (Vaccinium myrtillus L.) Fruits

Effects of light emitting diode irradiation at night on abscisic acid metabolism and anthocyanin synthesis in grapes in different growing seasons

ABA Transport and Distribution in Relation to Its Function in Plants

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