Changes in testis of guinea pig after vasectomy

Hutson, James C.; Gardner, Paul J.; Lacy, Sushil S.

doi:10.1016/0090-4295(76)90460-x

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…Multinucleated giant cells and tubular atrophy were common in vasectomy‐induced spermatogenic damage, probably as a result of germ cell sloughing (Hutson et al. 1976; Urry et al.…”

Section: Discussionmentioning

confidence: 99%

“…Multinucleated giant cells and tubular atrophy were common in vasectomy-induced spermatogenic damage, probably as a result of germ cell sloughing (Hutson et al 1976;Urry et al 1976;Neaves, 1978;Perera, 1978;Tung & Alexander, 1980;Singh & Chakravarty, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In other mammals, however, there seemed always to be some degree of spermatogenic damage after vasectomy, although individual variation was considerable. Hutson et al (1976) reported that 13 of 30 vasectomized (for 1-6 months) guinea-pigs had spermatogenic damage. McDonald & Scothorne (1988) reported five (out of eight) normal vasectomized testes (weights 1.2-1.6 g) after vasectomy (for 6 months) in rats, but the other three vasectomized testes, atrophied with weights of 0.7-1.0 g, were not studied.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative (stereological) study of the effects of vasectomy on spermatogenesis in rabbits

et al. 2004

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Using stereological methods, especially the optical disector for unbiased estimation of nuclear number, our recent study demonstrated that long-term (6 or 12 months) vasectomy in the rhesus monkey had no significant effects on spermatogenesis (Peng et al. Reproduction 2002, 124, 847-856). This study aimed to determine the scenario in the rabbit using the same morphometric methodology. Three groups of normal male Japanese white rabbits (aged 4-5 months) were subjected to unilateral vasectomy; 10 days, 6 months and 12 months later both testes and epididymides were removed. Testicular and epididymal methacrylate-embedded sections were obtained for stereology.Vasectomy-induced damage to spermatogenesis was observed, primarily sloughing of spermatogenic cells with a greater reduction in the number of advanced (adluminal) cells. The damage was most severe at 10 days, occurring in all the testes on the vasectomized side and involving sloughing of even type A spermatogonia, the number of which returned to normal at 6 and 12 months. Damage was less severe at 6 and 12 months, being found in half of the testes of the vasectomy side, in which the total numbers of later germ cell types were 24.0-59.1% (spermatocytes) and 0.3-11.6% (spermatids) of control at 6 months, and 20.1-22.1% (spermatocytes) and 0.4-12.0% (spermatids) of control at 12 months. By contrast, Sertoli cell number per testis was unchanged following vasectomy in any group.Epididymis on the vasectomy side, especially at 10 days and 6 months, appeared larger than on the contralateral side, but this difference was not statistically significant, and no sperm granuloma was seen in the epididymis.

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“…Multinucleated giant cells and tubular atrophy were common in vasectomy‐induced spermatogenic damage, probably as a result of germ cell sloughing (Hutson et al. 1976; Urry et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Quantitative (stereological) study of the effects of vasectomy on spermatogenesis in rabbits

et al. 2004

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“…vasectomy is known to cause injury to the testis and epididymis in the rabbit (Flickinger, 1975a,b;Bedford, 1976;Alexander and Tung, 1977), as well as a number of other species (Grewel and Sachan, 1968;Hafs et a!., 1974;Hutson, 1976;Perera, 1978). However, little attention has been given to the idea of diverting sperm for purposes of male fertility control.…”

Section: Restoration Of Fertilitymentioning

confidence: 98%

Changes in the Testes and Epididymides of Rabbits Following Long Term Vasectomy or Vasocystostomy: Correlation with Results of Vasovasostomy

Hooker¹

1980

Biology of Reproduction

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The purpose of the present study was to compare the effect of vasectomy (sperm obstruction) with vasocystostomy (sperm diversion) on the testes and epididymides of rabbits and no correlate pathologic changes with success or failure of vasovasostomy no restore fertility.Vasovasosnomy restored fertility no 36% of rabbits following vasectomy and no 100% of those in which sperm had been previously diverted by vasocysnosnomy. Pathologic changes were found in 73% of testes and 42% of epididymides following sperm obstruction, but there were no changes in the testis and only rare changes in the epididymis following sperm diversion.It is concluded that vasocystosnomy is a more physiological form of surgical contraception than conventional vasectomy in the rabbit.

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“…To date, there is no unanimity in opinion among authors. While some did not find alterations in rat (Flickinger, 1972; McDonald and Scothorne, 1988), rabbit (Paufler and Foote, 1969; Flickinger, 1975a,b), and monkey (Chapman et al, 1978; Hadley and Dym, 1983), others reported marked changes in mouse (Singh and Chakravarty, 2000), rat (Flickinger et al, 1986, 1987, 1990; McDonald et al, 1996; Sarrat et al, 1996; Aydos et al, 1998; Whyte et al, 2000), hamster (Hamasaki et al, 1991; Sun et al, 1992), guinea pig (Hutson et al, 1976; Tung and Alexander, 1977), rabbit (Swanson and Hafs, 1969; Hooker, 1980), dog (Antypas et al, 1994), and monkey (Lohiya et al, 1987).…”

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confidence: 98%

Effect of Vasectomy on Sperm Nuclear Chromatin Condensation in the Rabbit

Steger

Slavov

Failing³

et al. 2005

Journal of Andrology

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Histone-to-protamine exchange in haploid spermatids is known to play a central role for male fertility. The present study investigates, for the first time, the effects of vasectomy on the expression of protamines in the rabbit. During normal spermatogenesis, protamine-1 and protamine-2 mRNA were expressed from step 5 round spermatids to step 11 elongated spermatids. In unilaterally vasectomized animals, control testes revealed normal spermatogenesis with normal protamine expression, while vasectomized testes exhibited both normal spermatogenesis and spermatogenic arrest. Some testes with normal spermatogenesis revealed delayed expression of both protamine-1 and protamine-2. Furthermore, multinucleated round spermatids were a regular finding in these testes. In both treated and untreated animals, a higher percentage of spermatozoa from the cauda epididymis had highly condensed chromatin when compared with those from the testis. The percentage of spermatozoa with highly condensed chromatin from testes and epididymides from the vasectomized side of treated animals remained unchanged from controls. As the integrity of nuclear chromatin is important for oocyte fertilization, especially in intracytoplasmic sperm injection (ICSI), where most of the natural selection mechanisms are bypassed, our data add valuable information for the treatment of infertility by ICSI, showing that vasectomy may affect nuclear chromatin integrity of testicular spermatids but not epididymal spermatozoa. Microsurgical epididymal sperm aspiration (MESA), therefore, may be superior to testicular sperm extraction (TESE) in vasectomized patients.

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Changes in testis of guinea pig after vasectomy

Cited by 9 publications

References 8 publications

Quantitative (stereological) study of the effects of vasectomy on spermatogenesis in rabbits

Quantitative (stereological) study of the effects of vasectomy on spermatogenesis in rabbits

Changes in the Testes and Epididymides of Rabbits Following Long Term Vasectomy or Vasocystostomy: Correlation with Results of Vasovasostomy

Effect of Vasectomy on Sperm Nuclear Chromatin Condensation in the Rabbit

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