Changes in Tear Proteome After Acupuncture Treatment in Dry Eye

Tong, Louis; Zhou, Lei; Koh, Siew Kwan; Gao, Yan; Htoon, Hla Myint; Hou, Aihua; Lu, Dunyue; Wei, Qi-Ping; Lim, Pat

doi:10.2147/opth.s334942

Cited by 5 publications

(11 citation statements)

References 9 publications

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“…The negatively correlated proteins in the AT group are involved in gluconeogenesis and actin-related biological processes. This indicates that the potential mechanism of acupuncture treatment for VDT-related dry eye may be associated with the activation of B-cell-mediated immunity processes, which agrees with the findings of Liu et al [9] and Tong et al [23] . Furthermore, the mechanism of artificial tear treatment for VDT-related dry eye may involve biological processes such as protein folding, glycolysis, endosomal, and actin cytoskeleton, among others.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, we performed tear proteome testing on a total of 120 samples from 60 patients before and after treatment using MS-based, DIA quantitative proteomics and identified 7502 proteins in total, which significantly expanded the depth of identification of the tear proteome [9,15,16] . Among them, 4717 in the AC group and 4539 in the AT group were identified together before and after treatment, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The tear proteome can provide insight into the etiology and mechanisms associated with the disease at a molecular level and present a possible source of biomarkers of DED [15] . Moreover, the tear proteome has been used to investigate the therapeutic mechanisms of acupuncture [9,16] , such that it may be a good method for investigating the therapeutic mechanisms of acupuncture. In this study, we aimed to investigate whether acupuncture is effective in treating VDT-related dry eye by a randomized controlled trial, and to identify the possible mechanisms, by comparing the tear proteomics before and after acupuncture treatment.…”

Section: Introductionmentioning

confidence: 99%

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Effect of acupuncture on tear proteomics in patients with VDT-related dry eye

tang,

Tang,

Jiang

et al. 2023

Preprint

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Background Acupuncture is widely used to treat dry eye disease (DED) and improved dry eye signs, but the effect has not been reported treating video display terminal (VDT)-related dry eye. Meanwhile, the mechanism of acupuncture on VDT-related dry eye remains unknown, and the treatments of DED are often associated with an altered tear protein composition. Methods Sixty patients with VDT-related dry eye were randomly and equally allocated to the acupuncture (AC group) or the artificial tear groups (AT group). Treatment involved acupuncture or artificial tear for 4 weeks. Tear samples were collected after baseline examination of clinical parameters, including Ocular Surface Disease Index (OSDI) scores, tear film break-up time (TFBUT), and Schirmer I test (SIT) before and after treatment. Tear samples were subjected to proteomic identification and analysis by independent acquisition. Results The OSDI scores were significantly different between the AC and AT groups at the end of the study period when performing repeated measurement analysis of before, after, and 4 weeks after treatment by repeated measurement analysis (p = 0.017), whereas the TFBUT and SIT were not significantly different between the groups. In the AC group, the OSDI scores, TFBUT, and SIT were significantly improved at 4 weeks after treatment by one-way analysis (p = 0.001, p = 0.033, p = 0.033, respectively); whereas, there was no significant improvement in the AT group at that time. A total of 7502 proteins were identified by data-independent acquisition. Proteome changes pre- and post-treatment in the AC group were associated with B cell-related immune processes, inflammation, glycolysis, and actin cytoskeleton, and, accordingly, the protein expression levels of actin-related protein 2/3 complex, subunit 3, pyruvate kinase M, gelsolin, and lactate dehydrogenase A, among others, differed significantly between the effective and ineffective patients based on the OSDI scores within the AC group. Furthermore, the proteins hexosaminidase A and mannose-binding lectin 1 could prospectively predict whether acupuncture treatment was effective, based on an inverse convolution analysis and machine learning algorithms. Conclusions Acupuncture, relative to artificial tear, can provide greater improvement in the clinical symptoms of VDT-related dry eye. The mechanism of acupuncture in VDT-related dry eye treatment may be associated with glycolysis- and actin cytoskeleton remodeling-mediated inflammatory and immune processes. Additionally, hexosaminidase A and mannose-binding lectin 1 are biomarkers for predicting the efficacy of acupuncture for VDT-related dry eye. Key words: Acupuncture; VDT-related dry eye; Tear proteomics; Glycolysis; Actin cytoskeleton; Biomarkers.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of acupuncture on tear proteomics in patients with VDT-related dry eye

tang,

Tang,

Jiang

et al. 2023

Preprint

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“…Higher levels of these proteins are thought to be released from ocular surface epithelial cells as a result of evaporative dry eye[ 68 ] and were related to the keratinization of the meibomian gland ducts. [ 69 ]…”

Section: Dry Eye Disease Associationsmentioning

confidence: 99%

Tear proteomics in dry eye disease

Kannan

Das

Shetty

et al. 2023

Indian Journal of Ophthalmology

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Dry eye disease (DED) is a multi-factorial ocular surface condition driven by compromised ocular lubrication and inflammation which leads to itching, dryness, and vision impairment. The available treatment modalities primarily target the acquired symptoms of DED including tear film supplements, anti-inflammatory drugs, mucin secretagogues, etc., However, the underlying etiology is still an area of active research, especially in regard to the diverse etiology and symptoms. Proteomics is a robust approach that has been playing major role in understanding the causative mechanism and biochemical changes in DED by identifying the changes in protein expression profile in tears. Tears are a complex fluid composed of several biomolecules such as proteins, peptides, lipids, mucins, and metabolites secreted from lacrimal gland, meibomian gland, cornea, and vascular sources. Over the past two decades, tears have emerged as a bona-fide source for biomarker identification in many ocular conditions because of the minimally invasive and simple sample collection procedure. However, the tear proteome can be altered by several factors, which increases the complexity of the approach. The recent advancements in untargeted mass spectrometry-based proteomics could overcome such shortcomings. Also, these technological advancements help to distinguish the DED profiles based on its association with other complications such as Sjogren’s syndrome, rheumatoid arthritis, diabetes, and meibomian gland dysfunction. This review summarizes the important molecular profiles found in proteomics studies to be altered in DED which have added to the understanding of its pathogenesis.

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“…The tear proteome can provide insights into the etiology and mechanisms associated with DED at the molecular level and present a possible source of biomarkers . Moreover, as the tear proteome has been used to investigate the therapeutic mechanisms of acupuncture, , it may be a good method for investigating the therapeutic mechanisms of acupuncture. In this study, we aimed to investigate whether acupuncture is effective in treating VDT-related dry eye by a randomized controlled trial and to identify the possible mechanisms by comparing tear proteomics before and after acupuncture treatment.…”

Section: Introductionmentioning

confidence: 99%

Effect of Acupuncture on Tear Proteomics in Patients with Video Display Terminal-Related Dry Eye

Tang,

Lyu

et al. 2024

J. Proteome Res.

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Acupuncture is widely used to treat dry eye disease (DED), but its effect has not been reported in treating video display terminal (VDT)-related dry eye, and the mechanism of acupuncture on VDT-related dry eye is also unknown. In our study, the tear proteome was compared with identifying possible mechanisms and biomarkers for predicting acupuncture effectiveness in VDT-related dry eye. The results showed that the ocular surface disease index scores were significantly different between the acupuncture group (AC group) and artificial tears group (AT group) at the end of the study, whereas tear film breakup time (TFBUT) and Schirmer I test (SIT) were not significantly different between the groups. Proteome changes pre- and post-treatment in the AC group were associated with B cell-related immune processes, inflammation, glycolysis, and actin cytoskeleton. Furthermore, the proteins hexosaminidase A and mannose-binding lectin 1 could prospectively predict whether acupuncture treatment was effective. Therefore, we believe that acupuncture can provide greater improvement in the clinical symptoms of VDT-related dry eye than artificial tears. The mechanism of acupuncture in VDT-related dry eye treatment may be associated with glycolysis- and actin cytoskeleton remodeling-mediated inflammatory and immune processes. Additionally, hexosaminidase A and mannose-binding lectin 1 are biomarkers for predicting the efficacy of acupuncture for VDT-related dry eye.

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Changes in Tear Proteome After Acupuncture Treatment in Dry Eye

Cited by 5 publications

References 9 publications

Effect of acupuncture on tear proteomics in patients with VDT-related dry eye

Effect of acupuncture on tear proteomics in patients with VDT-related dry eye

Tear proteomics in dry eye disease

Effect of Acupuncture on Tear Proteomics in Patients with Video Display Terminal-Related Dry Eye

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