Changes in synovial fluid and serum concentrations of cartilage oligomeric matrix protein over 5 years after anterior cruciate ligament rupture: an exploratory analysis in the KANON trial

Struglics, A.; Larsson, S.; Pramhed, Anna; Frobell, Richard; Swärd, Per

doi:10.1016/j.joca.2018.06.005

Cited by 21 publications

(42 citation statements)

References 49 publications

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“…It recruits principally monocytes and to a lesser extent, memory T cells and dendritic cells to sites of inflammation. CCL2 is expressed in the synovial sublining cells of OA joints, and has been found in synovial fluid of OA knee joints 8 , 9 and following acute traumatic injury 10 , 11 . Ccl2 is strongly and rapidly (<6 h) induced in whole joints upon surgical joint destabilisation in the mouse 12 .…”

Section: Introductionmentioning

confidence: 99%

CCL2 and CCR2 regulate pain-related behaviour and early gene expression in post-traumatic murine osteoarthritis but contribute little to chondropathy

Zarebska

Chanalaris

Driscoll

et al. 2017

Osteoarthritis and Cartilage

105

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SummaryObjectiveThe role of inflammation in structural and symptomatic osteoarthritis (OA) remains unclear. One key mediator of inflammation is the chemokine CCL2, primarily responsible for attracting monocytes to sites of injury. We investigated the role of CCL2 and its receptor CCR2 in experimental OA.DesignOA was induced in 10 weeks old male wild type (WT), Ccl2−/− and Ccr2−/− mice, by destabilisation of the medial meniscus (DMM). RNA was extracted from whole joints at 6 h and 7 days post-surgery and examined by reverse transcription polymerase chain reaction (RT-PCR). Gene expression changes between naïve and DMM-operated mice were compared. Chondropathy scores, from mice at 8, 12, 16 and 20 weeks post DMM were calculated using modified Osteoarthritis Research Society International (OARSI) grading systems. Changes in hind paw weight distribution, as a measure of pain, were assessed by Linton incapacitance.ResultsAbsence of CCL2 strongly suppressed (>90%) selective inflammatory response genes in the joint 6 h post DMM, including arginase 1, prostaglandin synthase 2, nitric oxide synthase 2 and inhibin A. IL6, MMP3 and tissue inhibitor of metalloproteinase 1 were also significantly suppressed. Similar trends were also observed in the absence of CCR2. A lower average chondropathy score was observed in both Ccl2−/− and Ccr2−/− mice at 12, 16 and 20 weeks post DMM compared with WT mice, but this was only statistically significant at 20 weeks in Ccr2−/− mice. Pain-related behaviour in Ccl2−/− and Ccr2−/− mice post DMM was delayed in onset.ConclusionThe CCL2/CCR2 axis plays an important role in the development of pain in murine OA, but contributes little to cartilage damage.

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Section: Introductionmentioning

confidence: 99%

CCL2 and CCR2 regulate pain-related behaviour and early gene expression in post-traumatic murine osteoarthritis but contribute little to chondropathy

Zarebska

Chanalaris

Driscoll

et al. 2017

Osteoarthritis and Cartilage

105

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show abstract

“…Since the seminal studies demonstrating release of COMP into the joint environment after injury 8,9 ; a number of studies have found that synovial fluid and/or serum COMP is increased with joint injury and/or the progression of OA (in both cross-sectional and longitudinal studies) 16,17,30 . It is also important to note that not all studies have demonstrated that COMP increase after joint injury and/or correlates with structural changes within the joint 31 .…”

Section: Discussionmentioning

confidence: 99%

“…Daily calibration of the scanner employed a phantom spine containing composites of bone, fat and lean tissue. Participants were positioned in supine on the scanner bed according to the manufacturer's recommendations and instructed to remain as still as possible for the duration of the scan 16 . All procedures were consistent with the official positions of the International Society for Clinical Densitometry 26 .…”

Section: Fat Mass Indexmentioning

confidence: 99%

Serum cartilage oligomeric matrix protein (COMP) expression in individuals who sustained a youth sport-related intra-articular knee injury 3–10 years previously and uninjured matched controls

Laudon

Whittaker

Ren

et al. 2019

Osteoarthritis and Cartilage

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s u m m a r yObjective: This study investigates the relationship between a youth sport-related intra-articular knee injury and cartilage oligomeric matrix protein (COMP), a biomarker of cartilage turnover. Design: Participants included a sub-sample (n ¼ 170) of the Alberta Youth Prevention of Early Osteoarthritis (PrE-OA) study group. Specifically, 85 individuals with a 3e10 year history of sport-related intraarticular knee injury and 85 age, sex and sport-matched controls. COMP levels were investigated in serum. Between group differences in COMP levels, COMP fragmentation patterns and, the relationship between serum COMP and clinical outcomes (i.e., Magnetic Resonance Imaging (MRI) Osteoarthritis Knee Score; MOAKS, Knee Osteoarthritis Outcome Score; KOOS, Fat mass index; FMI) were examined. Results: Participant median age was 22.3 years (range 16e26) and 63% were female. Although there was no difference in COMP levels between previously injured and uninjured females, previously injured males demonstrated an~15% greater (171.5 ng/ml, 95% CI 11.0e428.0, P ¼ 0.04) serum COMP level than uninjured males. However after controlling for FMI, this difference was absent. Within the injured participants, COMP levels were associated with MOAKS SYNOVITIS and FMI. Furthermore, COMP fragmentation patterns were distinct between injured and uninjured individuals. Conclusions: In this study group, serum COMP levels were greater in injured males, but not females, compared to matched controls. However, after controlling for FMI, no differences in COMP were observed. A unique COMP fragmentation pattern was observed in injured vs uninjured participants. These results further the hypothesis that COMP levels and/or degradation of the protein may be a marker of cartilage injury which could predispose to later OA.

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“…It is well known that the altered joint milieu in addition to cartilage damage and meniscal loss is associated with the development of osteoarthritis. [11][12][13] Some of those changes probably get relevant in the long run. 11 The disorder of collagen turnover following ACL-R has also been investigated.…”

Section: See Related Article On Page 996mentioning

confidence: 99%

“…[11][12][13] Some of those changes probably get relevant in the long run. 11 The disorder of collagen turnover following ACL-R has also been investigated. The study suggests that type II collagen degradation may be greater in the ACL-R joint compared with type II collagen synthesis at higher joint strain.…”

Section: See Related Article On Page 996mentioning

confidence: 99%

Editorial Commentary: No Difference in Knee Osteoarthritis After Single-Bundle Versus Double-Bundle Anterior Cruciate Ligament Reconstruction

Mayr

Stoehr

2019

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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Changes in synovial fluid and serum concentrations of cartilage oligomeric matrix protein over 5 years after anterior cruciate ligament rupture: an exploratory analysis in the KANON trial

Cited by 21 publications

References 49 publications

CCL2 and CCR2 regulate pain-related behaviour and early gene expression in post-traumatic murine osteoarthritis but contribute little to chondropathy

CCL2 and CCR2 regulate pain-related behaviour and early gene expression in post-traumatic murine osteoarthritis but contribute little to chondropathy

Serum cartilage oligomeric matrix protein (COMP) expression in individuals who sustained a youth sport-related intra-articular knee injury 3–10 years previously and uninjured matched controls

Editorial Commentary: No Difference in Knee Osteoarthritis After Single-Bundle Versus Double-Bundle Anterior Cruciate Ligament Reconstruction

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