Changes in surface markers of human mesenchymal stem cells during the chondrogenic differentiation and dedifferentiation processes in vitro

Abstract

Objective. To investigate surface markers showing specific changes during the chondrogenic differentiation and dedifferentiation of human mesenchymal stem cells (MSCs).Methods. Human MSCs from adult bone marrow were subjected to chondrogenic differentiation in 3-dimensional (3-D) alginate culture with or without transforming growth factor ␤3 (TGF␤3) for 2 weeks, followed by dedifferentiation in monolayer for 1 week. Surface antigens were selected from those previously reported to show changes in expression during dedifferentiation of human articular chondrocytes (HACs).Results. Flow cytometry was used to identify 3 groups of surface antigens with differential expression patterns that were quite different from those previously reported on HACs. Two groups of antigens were expressed at high levels on human MSCs. The expression of the first group of antigens (CD44, CD58, CD81, CD90, CD105, and CD166) was decreased reversibly by the 3-D alginate culture and irreversibly in the presence of TGF␤3, except for CD81, which showed reversible changes regardless of TGF␤3. The expression of the second group of antigens (CD49c, CD49e, and CD151) was decreased during chondrogenic differentiation only in the presence of TGF␤3. During all experimental stages, the expression of the third group of antigens (CD14, CD26, CD49f, CD54, CD106, CD119, and CD140a) was maintained at low levels (expressed on <30% of cells), although with some fluctuations.Conclusion. We speculate that the second group of surface antigens could be negative markers for chondrogenic differentiation of human MSCs.

Differences in Surface Marker Expression and Chondrogenic Potential among Various Tissue-Derived Mesenchymal Cells from Elderly Patients with Osteoarthritis

“…In agreement with Mareddy et al [2007] and Astori et al [2007], who observed low expression of CD271 in MSCs from adipose tissue, we observed that the expression of CD271 was below 16% in MSCs from all the tissues analysed. Grogan et al [2007] showed that CD44, CD49c and CD151 are expressed at high levels in chondrocytes with chondrogenic potential, and these molecules have also been described as useful flow cytometry markers for human bone marrow-derived MSCs [Lee et al, 2009]. In agreement with these results, we observed expression of these markers in MSCs from all tissues analysed.…”

“…We found that approximately 30% of cells expressed CD54 (ICAM-1), in keeping with the percentage observed by Sakaguchi et al [2005] and Lee et al [2009] in human bone marrow-derived MSCs. CD54 expression was higher in subcutaneous fat-derived MSCs ( fig.…”

Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation

“…In chondrogenic medium, Thy1 mRNA level decreases deeply since day 7 of differentiation. These results are in accordance to the literature, where it was demonstrated that many MSCs markers among them Thy1 were decreased during 7 days of chondrogenic differentiation [56]. For nucleostemin and endoglin, mRNA level analysis did not show difference in cell in chondrogenic medium compared to MSCs.…”

Expression Patterns and Function of Chromatin Protein HMGB2 during Mesenchymal Stem Cell Differentiation

“…4D). In contrast, CD105, a marker of human MSC that decreases in chondrogenesis culture (36), was higher in LV/HMGB2 control group. These findings suggest that sustained expression of HMGB2 in MSC blocks chondrogenic dif- ferentiation and maintains immature status of MSC even after chondrogenesis was induced.…”