Changes in superoxide radical and lipid peroxide formation in the brain, heart and liver during the lifetime of the rat

Sawada, Masaaki; Carlson, John C.

doi:10.1016/0047-6374(87)90057-1

Cited by 233 publications

(100 citation statements)

References 33 publications

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“…Some studies report an increased oxidant generation with age [23], whereas others, including Drew and coworkers, using a SSM-enriched preparation, observed no change in mitochondrial oxidant production [24][25][26]. Apparent discrepancies in the extent of age-related changes in mitochondrial oxidant appearance and oxidative stress may reflect the differences in mitochondrial isolation procedures and resultant heterogeneity in the amount of IFM and SSM obtained in sample preparations.…”

Section: Discussionmentioning

confidence: 99%

Two subpopulations of mitochondria in the aging rat heart display heterogenous levels of oxidative stress

Suh

Heath

Hagen

2003

Free Radical Biology and Medicine

111

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Cardiac mitochondria are composed of two distinct subpopulations: one beneath the sarcolemma (subsarcolemmal mitochondria: SSM), and another along the myofilaments (interfibrillary mitochondria: IFM). Previous studies suggest a preferential loss of IFM function with age; however, the age-related changes in oxidative stress in these mitochondrial subpopulations have not been examined. To this end, the changes in mitochondrial antioxidant capacity, oxidant output, and oxidative damage to Complex IV in IFM and SSM from young and old rats were studied. Results show no apparent differences in any parameters examined between IFM and SSM from young rats. However, relative to young, only IFM from old rats had a significantly higher rate of oxidant production and a decline in mitochondrial ascorbate levels and GSH redox status. The age-related decline in mitochondrial antioxidant capacity in IFM was accompanied by a marked loss in glutaredoxin and GSSG reductase activities, suggesting a diminished reductive capacity in IFM with age. Moreover, the loss in Complex IV activity was limited to the IFM of old rats, which was accompanied by a 4-fold increase in 4-hydroxynonenal-modified Complex IV. Thus, mitochondrial decay is not uniform and further indicates that myofibrils may be uniquely under oxidative stress in the aging heart.

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Section: Discussionmentioning

confidence: 99%

Two subpopulations of mitochondria in the aging rat heart display heterogenous levels of oxidative stress

Suh

Heath

Hagen

2003

Free Radical Biology and Medicine

111

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“…Superoxide production in mitochondria increases with advancing age [52]. In man about 2 × 102° molecules 02 are reduced per g of tissue per day.…”

Section: Mitochondria and Oxygenmentioning

confidence: 99%

Do mitochondrial DNA fragments promote cancer and aging?

Richter

1988

FEBS Letters

211

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Reactive oxygen species are important in carcinogenesis, diseases, and aging, probably through oxidative damage of DNA. Our understanding of this relationship at the molecular level is very sketchy. It has recently been found that in mitochondria oxidative DNA damage is particularly high and may not be repaired efficiently. I propose that oxidatively generated DNA fragments escape from mitochondria and become integrated into the nuclear genome. This may transform cells to a cancerous state. Time‐dependent nuclear accumulation of mitochondrial DNA fragments may progressively change the nuclear information content and thereby cause aging. This proposal can be tested experimentally.

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“…1988). To date however, the oxidation of spin trapping agents by 0 2 (measured by ESR spectroscopy) is the only direct measure for basal levels of oxygen reactive species employed in neuronal tissues (Sawada and Carlson, 1987). The intracellular trapping of DCFH, and its subsequent oxidation to DCF has been reported as a direct measure of oxygen reactive species formation in non-neuronal tissue (Scott ct al., 1988;Szejda et al, 1984;Bass et al, 1983 ).…”

Section: ()Jscussionmentioning

confidence: 99%

“…Using electron spin resonance techniques, brain in flow cytometric studies. DCFHmitochondrial superoxide radical formation has been DA is a stable, non-fluorescent molecule that readily shown to be age-related (Sawada and Carlson, 1987). crosses cell membranes, and is hydrolyzed by intraTraditionally, free-radical initiated lipid peroxida-cellular esterases to non-fluorescent 2',7'-dichlorotion has been estimated with techniques such as the fluorescin (DCFH) (Bass et al, 1983).…”

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confidence: 99%

Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes

LeBel

Bondy

1990

Neurochemistry International

303

151

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Abstract-The formation of oxygen reactive species in response to oxidative stimuli was measured in rat synaptosomes. Studies employed the non-fluorescent probe 2', 7' -dichlorofluorescin diacetate (DCFH-DA), which after de-esterification is oxidized in the presence of oxygen reactive species to the highly fluorescent 2',7'-dichlorofluorescein (DCF). Oxygen reactive species formation, as measured by DCF fluorescence, was stimulated by ascorbate and/or FeS0 4 , and xanthine/xanthine oxidase under various buffering conditions. These agents all increased DCF formation in Tris, HEPES and phosphate buffer. Ascorbate also stimulated the formation of DCF in a concentration-dependent manner. The presence of Ca 2 + in HEPES buffer did not enhance or diminish the effects of ascorbate/FeS0 4 on DCF formation. Deferoxamine inhibited the ascorbate/FeS0 4 -induced stimulation of DCF formation, but xanthine/xanthine oxidaseinduced stimulation was not affected by pretreatment with superoxide dismutase. Results indicate that DCF fluorescence is a sensitive, quantitative and direct measure of oxygen reactive species formation in synaptosomes, providing a rapid method for investigating early neuronal events that occur during oxidative stress.

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Changes in superoxide radical and lipid peroxide formation in the brain, heart and liver during the lifetime of the rat

Cited by 233 publications

References 33 publications

Two subpopulations of mitochondria in the aging rat heart display heterogenous levels of oxidative stress

Two subpopulations of mitochondria in the aging rat heart display heterogenous levels of oxidative stress

Do mitochondrial DNA fragments promote cancer and aging?

Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes

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