Changes in Storage Protein Components of Developing Soybean Seeds (Glycine max var. Enrei).

Kondo, Kimio; Sugimoto, Tsuneaki; Saio, Kyoko

doi:10.1080/00021369.1986.10867437

Cited by 3 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, α and α′ subunits of 7S protein appeared first (15 DAF), followed by the β subunit of 7S protein and acidic and basic subunits of 11S proteins (data not shown). Consistent with results of several studies (Goldberg et al, 1981; Meinke et al, 1981; Kondo et al, 1986), accumulation of all seed storage protein subunits was rapid from 15 to 45 DAF (data not shown). The percentage of 11S protein subunits increased from 15 to 60 DAF, while the percentage of 7S protein subunits decreased during this time period for all varieties (data not shown).…”

Section: Resultssupporting

confidence: 91%

Regulation of Seed Protein Concentration in Soybean by Supra‐Optimal Nitrogen Supply

et al. 2000

View full text Add to dashboard Cite

nificant barriers to development of high-protein commercial cultivars. The physiological and biochemical basis for increased seed proteinSoybean SPC is inherited as a quantitative trait (Burconcentrations (SPC) observed in restriction-index, recurrent-selec- ton, 1987) and influenced by environmental effects tion breeding programs with soybean [Glycine max (L.) Merr.] are poorly understood. The hypothesis that soybean SPC is regulated by (Burton, 1988). Generally the trait is much less influthe supply of nitrogenous substrates available to the seed was evaluenced by the genotype of the embryo than by the genoated. Effects of supra-optimal external N on seed storage protein type on which the seeds develop (Singh and Hadley, accumulation, amino acid concentration and composition in leaves 1968). This suggests that whole plant processes such as and seeds at R5, and levels of specific storage protein subunits were N acquisition, translocation, and mobilization of C and measured. Genotypes with different SPC (NC 107, normal; N87-984-N are important in the determination of seed protein 16, intermediate; and NC 111, high) were grown in controlled-environconcentration. ment chambers and supplied with 30 mM N as NH 4 NO 3 from V5 to Brim and Burton (1979) used recurrent selection to maturity or from R5 to maturity. Control plants received 10 mM N increase SPCs in two populations (IA and IIA). Carter throughout the growth cycle. Relative to control, supra-optimal N et al. (1982) examined relationships between N accumuincreased SPC of NC 107 and N87-984-16 by an average of 28%. Greater enhancement of protein accumulation than of dry matter lation and distribution and SPC in high and low seed accumulation in the seed resulted in SPCs of 460 to 470 g kg Ϫ1 , germplasm from both populations. They observed that which are appreciably greater than concentrations observed for these high protein germplasm from population IA accumucultivars grown in the field. Supra-optimal N also increased SPC of lated more total N before reproductive development the high protein line (NC 111) by 15%, but this increase resulted than high seed protein germplasm in population IIA. entirely from a decrease in yield. Supra-optimal N supplied to NC Selected high and low seed protein lines derived from 107 and N87-984-16 from V5 until R5 increased total free amino acid Cycle 0 and advanced cycles of selection for both popuconcentrations in seeds and leaves at R5 by an average of 21 and lations were evaluated for vegetative N accumulation 46%, respectively. Enhanced accumulation of the ␤ subunit of ␤ prior to reproductive growth and vegetative N mobilizaconglycinin which does not contain methionine and cysteine accounted tion to seed during reproductive growth (Burton et al., for the increase in SPC. While enhanced N availability increased the SPC of a normal protein line into the high range, availability of sulfur 1995). The authors concluded that the high protein conamino acids in the developing seed determined which storage protein centration trait re...

show abstract

Section: Resultssupporting

confidence: 91%

Regulation of Seed Protein Concentration in Soybean by Supra‐Optimal Nitrogen Supply

et al. 2000

View full text Add to dashboard Cite

show abstract

“…With an increase in total protein content in soybean cotyledons, globulin will account for the largest proportion without large fluctuations. It is well known that globulins accumulate in soybean during seed ripening (Kondo et al, 1986). Likely, the accumulation capacity of globulins is strictly controlled to approximately 50% of the total accumulated proteins.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the contents and ratios of five fractionated proteins to elucidate the protein composition in soybean seeds

Sugiyama

Sakai

Ichinose

et al. 2023

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Selecting suitable soybean cultivars is important for food processing and exploring their endowment with desirable physiological functions. We applied the fractionation method previously established to compare soy protein composition among cultivars to promote such a selection. More than 95% of the proteins in soybean cotyledons were extracted from 13 soybean cultivars using a high‐concentration salt solution. The extracted proteins were fractionated into five fractions, namely oil body‐associated protein (OBAP), polar lipid‐associated protein (PLAP), globulins (11S and 7S), and whey by centrifugation after tuning the solubility behavior of the proteins with various solutions. Protein species in each fraction were analyzed by SDS‐PAGE. Protein content in the total extract and five fractions was quantified to characterize the protein composition of soybean cultivars. The correlation between the protein content of each fraction and the total protein in cotyledon was investigated. A strong positive correlation was found only for the 11S fraction (r = 0.82), followed by a positive correlation in the 7S fraction (r = 0.65). Thus, we surmised that the increased protein content in soybean was due to increased globulin content. Furthermore, the calculation of the average ratio of protein content in each fraction indicated the globulin fraction (7S and 11S) to be 52%, the lipophilic protein fraction (OBAP and PLAP) to be 33%, and the whey fraction to be 13%. The preparation method employed in this study is a promising tool for efficiently comparing the protein composition of soybean cultivars to evaluate the potential use of cultivars for food production.

show abstract

Dynamics of Seed Protein Biosynthesis in Two Soybean Genotypes Differing in Drought Susceptibility

Blanuša¹,

Stikić²,

Vucelić-Radović³

et al. 2000

Biol. plant.

View full text Add to dashboard Cite

Changes in Storage Protein Components of Developing Soybean Seeds (Glycine max var. Enrei).

Cited by 3 publications

References 3 publications

Regulation of Seed Protein Concentration in Soybean by Supra‐Optimal Nitrogen Supply

Regulation of Seed Protein Concentration in Soybean by Supra‐Optimal Nitrogen Supply

Evaluation of the contents and ratios of five fractionated proteins to elucidate the protein composition in soybean seeds

Dynamics of Seed Protein Biosynthesis in Two Soybean Genotypes Differing in Drought Susceptibility

Contact Info

Product

Resources

About