Changes in steroidogenic enzyme and steroidogenic acute regulatory protein messenger RNAs in ovarian follicles during ovarian development of rainbow trout (Oncorhynchus mykiss)

Nakamura, Ippei; Evans, Jennifer; Kusakabe, Makoto; Nagahama, Yoshitaka; Young, Graham

doi:10.1016/j.ygcen.2005.06.004

Cited by 78 publications

(67 citation statements)

References 38 publications

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“…Numerous previous studies have described high and increasing levels of expression of cyp19a1a through vitellogenesis, in cod (Breton et al, 2012), as well as other teleost species (e.g. Kumar et al, 2000;Nakamura et al, 2005;Rocha et al, 2009). In apparent contrast to these studies, we found the highest cyp19a1a transcript levels in spawning female cod.…”

Section: Discussioncontrasting

confidence: 99%

“…The low transcript levels of 20bhsd during final maturation and spawning in cod is in apparent contrast to some previous studies, which showed increasing expression during final maturation and spawning in other teleost species such as ayu (Plecoglossus altivelis), tilapia and rainbow trout (Nakamura et al, 2005;Senthilkumaran et al, 2002Senthilkumaran et al, , 2004Tanaka et al, 2002). On the other hand, (Bobe et al, 2003(Bobe et al, , 2004 did not find any changes in 20bhsd transcript levels in rainbow trout ovarian follicles during maturation and suggested involvement of other factors such as other enzymes or growth factors, and/or substrate availability.…”

Section: Discussioncontrasting

confidence: 99%

“…This is consistent with previously published work on cod (Goetz et al, 2004) as well as other species (Bobe et al, 2004;Kusakabe et al, 2002;Nakamura et al, 2005;Rocha et al, 2009). …”

Section: Discussionsupporting

confidence: 93%

“…This is in agreement with results reported in other species such as rainbow trout, tilapia and ayu (Nakamura et al, 2005;Senthilkumaran et al, 2002;Tanaka et al, 2002), where highest levels of 20bhsd transcripts were found in postovulatory follicles. Pinillos et al (2002) and Antonopoulou et al (2011) found that MIS plasma concentrations peaked two months after spawning in the tench (Tinca tinca) but, unlike the situation in huchen and carp (Miura et al, 2007), it was not correlated to onset of first meiosis.…”

Section: Discussionsupporting

confidence: 93%

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Effects of Dietary Arachidonic Acid on the Reproductive Physiology of Female Atlantic Cod (Gadus morhua L.).

Norberg¹,

Hamre²,

Andersson³

et al. 2008

Biology of Reproduction

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a b s t r a c tThe present study was designed to investigate potential effects of arachidonic acid (ARA) on the reproductive physiology of female Atlantic cod (Gadus morhua L.).Two-year old Atlantic cod of both sexes were equally distributed into eight sea cages after completion of their first spawning in May 2005. Four experimental groups were established and fed diets with different levels of ARA corresponding to 0.5, 1, 2 and 4% of total fatty acid. Ovarian growth and development was documented every month. Fatty acid composition was analysed in ovaries, liver and plasma at the beginning of the experiment, one month prior to spawning, and in spent fish, one month after spawning was completed. Plasma concentrations of estradiol-17b, testosterone and vitellogenin, and ovarian gene transcript levels of steroidogenic acute regulatory protein (star), P450aromatase (cyp19a1a) and 20b-hydroxy steroid dehydrogenase (20bhsd/cbr1) were monitored every month in fish fed the experimental diets and related to oocyte stage. Potential fecundity was calculated based on ovarian samples taken one month before onset of spawning.Ovarian and plasma ARA levels were highly correlated to dietary ARA levels. There was a net accumulation of ARA compared to other essential fatty acids in ovarian tissue that was reflected in a decrease in EPA:ARA ratio. Plasma concentrations of vitellogenin, estradiol-17b and testosterone and key gene transcript levels were affected by dietary ARA and stage of maturation. The results show that ARA has a significant influence on the reproductive physiology of female Atlantic cod.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

“…This is consistent with previously published work on cod (Goetz et al, 2004) as well as other species (Bobe et al, 2004;Kusakabe et al, 2002;Nakamura et al, 2005;Rocha et al, 2009). …”

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

See 2 more Smart Citations

Effects of Dietary Arachidonic Acid on the Reproductive Physiology of Female Atlantic Cod (Gadus morhua L.).

Norberg¹,

Hamre²,

Andersson³

et al. 2008

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…In adult ovaries of Atlantic croaker and rainbow trout, levels of CYP19A1 message were detected early in ovarian development and vitellogenesis with dramatically decreased expression later Nunez and Applebaum 2006). The CYP19A1 mRNA expression correlated with plasma estradiol levels in both channel catfish and rainbow trout (Kumar et al, 2000;Nakamura et al, 2005). Relatively few studies have investigated CYP19A1 expression during embryonic or ovarian development by in situ hybridization or immunohistochemistry.…”

Section: Introductionmentioning

confidence: 99%

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

Wang¹,

Willett²

2008

General and Comparative Endocrinology

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P450 aromatase (CYP19) is the terminal enzyme in the steroidogenic pathway and catalyzes the conversion of androgens to estrogens. Fundulus heteroclitus like other teleosts, express two CYP19 genes, CYP19A1 and CYP19A2. The expression of CYP19s in Fundulus was measured by in situ hybridization throughout development. In 90 dpf (day post-fertilization) fish and adult fish, CYP19A1 was expressed in the ooplasm of early stage I oocytes (primary growth stage). Expression of CYP19A1 was localized in the follicle cell layer of late stage I (previtellogenic stage) and stage II (vitellogenic stage) follicles, but by stage III (early maturational follicles) CYP19A1 expression was localized in the vitelline envelope. Overall, CYP19A1 oocyte membrane expression gradually declined from highest expression at late stage I to nondetectable levels by stage IV. Highest expression of CYP19A2 was detected in the brain including the hypothalamus from 4, 6, 8, 10, 14 dpf embryos, 90 dpf fry fish and adult fish brain. In females compared to males, there was higher CYP19A2 expression in olfactory bulb. In addition to the brain, there was strong CYP19A2 signal in adrenal/kidney cells in 6-14 dpf embryos. This work establishes the localization and constitutive expression of CYP19s in Fundulus which can then be compared with potential disruption of CYP19A1 and CYP19A2 expression and physiological consequences caused by environmental contaminants.

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In vitro conversion of sex steroids and expression of sex steroidogenic enzyme genes in amphioxus ovary

et al. 2008

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Sex steroids are essential hormones for reproduction in vertebrates. The existence of a sex steroidogenic pathway in invertebrates is controversial because cytochrome P450 (CYP) genes have not been detected in the genomes of an echinoderm and a urochordate. However, cloning and gene expressions of sex steroid-metabolizing enzymes have been reported in a cephalochordate. In this study, in vitro conversion of sex steroids from pregnenolone (P5) to progesterone (P4), from 17alpha-hydroxyprogesterone (17alpha-P4) to 17alpha, 20beta-dihydroxy-4-pregnen-3-one (17alpha, 20beta-P) and 17alpha, 20alpha-P, and from androstenedione (AD) to estrone (E1), estradiol-17beta (E2), and testosterone (T) were confirmed by an incubation experiment performed using (14)C-labeled precursors and mature ovarian homogenates of the amphioxus Branchiostoma belcheri. In amphioxus, the ability of immature ovaries to synthesize sex steroids was much lower than that of mature ovaries. Post-spawning, the mRNA of CYP11A significantly decreased in females. Transcripts of the CYP19 gene also declined in one-fourth of the females after spawning, although this trend was not supported statistically. The mRNAs of CYP17 and 17beta-HSD did not change before and after spawning. Our results indicate the presence of Delta(4) and another derivative pathway in the amphioxus ovary and suggest that the synthesis of sex steroids, particularly estrogen synthesis, may be low in females after spawning behavior.

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Changes in steroidogenic enzyme and steroidogenic acute regulatory protein messenger RNAs in ovarian follicles during ovarian development of rainbow trout (Oncorhynchus mykiss)

Cited by 78 publications

References 38 publications

Effects of Dietary Arachidonic Acid on the Reproductive Physiology of Female Atlantic Cod (Gadus morhua L.).

Effects of Dietary Arachidonic Acid on the Reproductive Physiology of Female Atlantic Cod (Gadus morhua L.).

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

In vitro conversion of sex steroids and expression of sex steroidogenic enzyme genes in amphioxus ovary

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