Changes in Soluble Protein Profile in Cotton Leaves Indicate Rubisco Damage after Treatment with Sumi-Alpha Insecticide

Abstract

The cotton plant suffers from many pests and due to its economic importance, the use of efficient but ecologically friendly pesticides is highly desirable. The insecticide Sumi-Alpha with active compound Esfenvalerate is widely used for cotton treatment in Uzbekistan. This insecticide is considered as moderately hazardous for different organisms of ecosystems, while more collected data and relevant research is a necessity for judgement of their safety and/or potential risk. Therefore, a comparative analysis of cotton leaves was performed to study the protein profile changes upon treatment with this insecticide. Our data showed elevated accumulation of fructose bisphosphate aldolase and degradation of RuBisCo in the treated tissue. Besides, a protein belonging to P-loop containing nucleoside triphosphate hydrolases superfamily exerted lower accumulation. Our results indicate that the applied pyrethroid pesticide possibly can affect photosynthesis performance and cause accumulation of simple saccharides which in turn might contribute to enhanced colonisation of sucking insects. Further molecular research on Sumi-Alpha effects on cotton is necessary for its safer use in agricultural practice.

Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables

“…As shown in Supplementary Figure S1C , Q/C and Q were linearly related, suggesting that MIP had a single binding site per template molecule. According to formulas ( 1 ) and ( 2 ), Qmax is 3.65 mg/g, and Kd is 47.62 mg/L, which are higher than those in the literature ( 34 ). By comparing the experimental results with those in other literature ( Table 1 ), we concluded that the MIPs have a high adsorption capacity.…”

“…Finally, the mixtures were centrifuged at 5,000 rpm for 3 min, and HPLC was used to determine the concentration in the supernatant to calculate the saturated absorption amount. The adsorption capacity Q (mg/g) of MIP to fenvalerate was calculated using formula ( 1 ). The maximum apparent capacity and binding site were determined by the Scatchard equation ( 2 ).…”

“…Pyrethroid pesticides have the broadest spectrum of high efficiency, low residue, moderate toxicity, and are biodegradable in plants. It effectively prevents and controls the storage of insect pests and has become the preferred pesticide in today's agriculture ( 1 , 2 ). Type II pyrethroid pesticides have good efficacy and stability and are widely used to protect crops against insects.…”

Determination of the Amount of Residual Pesticides in the Liver Tissue of Rats Poisoning With Haloxyphop-R-Methyl and Indosacarb Pesticides

“…The amount of residual pesticides in the liver of experimental rats A. It was conducted based on the methods recommended by Mamadrakhimov [1].…”

Recent Advances in the Recognition Elements of Sensors to Detect Pyrethroids in Food: A Review

“…The withdrawal of highly hazardous organophosphate pesticides from the world market over the last ten years has resulted in pyrethroid insecticides becoming the preferred alternative pesticides due to their effectiveness in pest control [ 1 , 2 ]. Pyrethroids have the broadest application, highest efficiency and lowest residue in addition to their moderate toxicity and biodegradability in plants.…”