Changes in soil microbial community functionality and structure in a metal-polluted site: The effect of digestate and fly ash applications

García‐Sánchez, Mercedes; García-Romera, Inmaculada; Cajthaml, Tomáš; Tlustoš, Pavel; Száková, Jiřina

doi:10.1016/j.jenvman.2015.07.042

Cited by 51 publications

(25 citation statements)

References 64 publications

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“…In the work of Galende et al [59], poultry manure had a better influence on soil metabolic activity than other manures as was reflected by significantly higher values of the AWCD, H’ and S indices. On the other hand, Garcia-Sánchez et al [60] did not observe any changes in the values of H' and metabolic richness (S) for Pb-, Ag- and Zn-contaminated soil in the presence of digestate and fly ash. Our statistical analysis indicated a strong positive correlation between acid and alkaline phosphatase activities with the bacterial and fungal biomass (p≤0.01) and decreasing concentrations of Zn and Cd (p≤0.05) in the treated soils.…”

Section: Discussionmentioning

confidence: 96%

Effects of Pulp and Na-Bentonite Amendments on the Mobility of Trace Elements, Soil Enzymes Activity and Microbial Parameters under Ex Situ Aided Phytostabilization

Wasilkowski¹,

Nowak²,

Płaza³

et al. 2017

PLoS ONE

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The objective of this study was to explore the potential use of pulp (by-product) from coffee processing and Na-bentonite (commercial product) for minimizing the environmental risk of Zn, Pb and Cd in soil collected from a former mine and zinc-lead smelter. The effects of soil amendments on the physicochemical properties of soil, the structural and functional diversity of the soil microbiome as well as soil enzymes were investigated. Moreover, biomass of Festuca arundinacea Schreb. (cultivar Asterix) and the uptake of trace elements in plant tissues were studied. The outdoor pot set contained the following soils: control soil (initial), untreated soil (without additives) with grass cultivation and soils treated (with additives) with and without plant development. All of the selected parameters were measured at the beginning of the experiment (t0), after 2 months of chemical stabilization (t2) and at the end of the aided phytostabilization process (t14). The obtained results indicated that both amendments efficiently immobilized the bioavailable fractions of Zn (87–91%) and Cd (70–83%) at t14; however, they were characterized by a lower ability to bind Pb (33–50%). Pulp and Na-bentonite drastically increased the activity of dehydrogenase (70- and 12-fold, respectively) at t14, while the activities of urease, acid and alkaline phosphatases differed significantly depending on the type of material that was added into the soil. Generally, the activities of these enzymes increased; however, the increase was greater for pulp (3.5-6-fold) than for the Na-bentonite treatment (1.3–2.2-fold) as compared to the control. Soil additives significantly influenced the composition and dynamics of the soil microbial biomass over the experiment. At the end, the contribution of microbial groups could be ordered as follows: gram negative bacteria, fungi, gram positive bacteria, actinomycetes regardless of the type of soil enrichment. Conversely, the shift in the functional diversity of the microorganisms in the treated soils mainly resulted from plant cultivation. Meanwhile, the highest biomass of plants at t14 was collected from the soil with Na-bentonite (6.7 g dw-1), while it was much lower in a case of pulp treatment (1.43–1.57 g dw-1). Moreover, the measurements of the heavy metal concentrations in the plant roots and shoots clearly indicated that the plants mainly accumulated metals in the roots but that the accumulation of individual metals depended on the soil additives. The efficiency of the accumulation of Pb, Cd and Zn by the roots was determined to be 124, 100 and 26% higher in the soil that was enriched with Na-bentonite in comparison with the soil that was amended with pulp, respectively. The values of the soil indices (soil fertility, soil quality and soil alteration) confirmed the better improvement of soil functioning after its enrichment with the pulp than in the presence of Na-bentonite.

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Section: Discussionmentioning

confidence: 96%

Effects of Pulp and Na-Bentonite Amendments on the Mobility of Trace Elements, Soil Enzymes Activity and Microbial Parameters under Ex Situ Aided Phytostabilization

Wasilkowski¹,

Nowak²,

Płaza³

et al. 2017

PLoS ONE

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“…These were in agreement with the finding by Carrasco et al (2010) which showed that cyc/prec ratio was positively correlated to the soluble contents of heavy metals. Garcia-Sánchez et al (2015) also reported that cy/pre ratio was increased under metal stress. In addition, high concentration of labile carbon from root exudates might also contribute to this.…”

Section: Effects Of S Alfredii Growth On Soil Microbial Community Stmentioning

confidence: 89%

Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

Yang

Zhang

Lin

et al. 2017

Environ Sci Pollut Res

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The recovery of microbial community and activities is crucial to the remediation of contaminated soils. Distance-dependent variations of microbial community composition and metabolic characteristics in the rhizospheric soil of hyperaccumulator during phytoextraction are poorly understood. A 12-month phytoextraction experiment with Sedum alfredii in a Cd-contaminated soil was conducted. A pre-stratified rhizobox was used for separating sub-layer rhizospheric (0-2, 2-4, 4-6, 6-8, 8-10 mm from the root mat)/bulk soils. Soil microbial structure and function were analyzed by phospholipid fatty acid (PLFA) and MicroResp™ methods. The concentrations of total and specified PLFA biomarkers and the utilization rates for the 14 substrates (organic carbon) in the 0-2-mm sub-layer rhizospheric soil were significantly increased, as well as decreased with the increase in the distance from the root mat. Microbial structure measured by the ratios of different groups of PLFAs such as fungal/bacterial, monounsaturated/saturated, ratios of Gram-positive to Gram-negative (GP/GN) bacterial, and cyclopropyl/monoenoic precursors and 19:0 cyclo/18:1ω7c were significantly changed in the 0-2-mm soil. The PLFA contents and substrate utilization rates were negatively correlated with pH and total, acid-soluble, and reducible fractions of Cd, while positively correlated with labile carbon. The dynamics of microbial community were likely due to root exudates and Cd uptake by S. alfredii. This study revealed the stimulations and gradient changes of rhizosphere microbial community through phytoextraction, as reduced Cd concentration, pH, and increased labile carbons are due to the microbial community responses.

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“…Biological oxidation of organic compounds is generally a dehydrogenation process, involving many enzymes, which are highly specific. The dehydrogenase activity indicates the overall soil microbial activity because they occur intracellular in all living cells (Pérez-de-Mora et al 2006;Melgar-Ramirez et al 2012;Garcia-Sánchez et al 2015b). In our study, applying digestate to Chernozem and Luvisol soils provoked a significant increase in the dehydrogenase activity in non-contaminated and Hg-contaminated samples (Fig.…”

Section: Soil Enzymes Activitiesmentioning

confidence: 99%

Organic and inorganic amendment application on mercury-polluted soils: effects on soil chemical and biochemical properties

García‐Sánchez

Klouza

Holečková

et al. 2016

Environ Sci Pollut Res

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On the basis of a previous study performed in our laboratory, the use of organic and inorganic amendments can significantly modify the Hg mobility in soil. We have compared the effectiveness of organic and inorganic amendments such as digestate and fly ash, respectively, reducing the Hg mobility in Chernozem and Luvisol soils differing in their physicochemical properties. Hence, the aim of this work was to compare the impact of digestate and fly ash application on the chemical and biochemical parameters in these two mercury-contaminated soils in a model batch experiment. Chernozem and Luvisol soils were artificially contaminated with Hg and then incubated under controlled conditions for 21 days. Digestate and fly ash were applied to both soils in a dose of 10 and 1.5 %, respectively, and soil samples were collected after 1, 7, 14, and 21 days of incubation. The presence of Hg in both soils negatively affected to processes such as nitrification, provoked a decline in the soil microbial biomass C (soil microbial biomass C (MBC)), and the microbial activities (arylsulfatase, and β-glucosaminidase) in both soils. Meanwhile, the digestate addition to Chernozem and Luvisol soils contaminated with Hg improved the soil chemical properties (pH, dissolved organic carbon (DOC), N (Ntot), inorganic-N forms (N-NH4 (+) and N-NO3 (-))), as consequence of high content in C and N contained in digestate. Likewise, the soil MBC and soil microbial activities (dehydrogenase, arylsulfatase, and β-glucosaminidase) were greatly enhanced by the digestate application in both soils. In contrast, fly ash application did not have a remarkable positive effect when compared to digestate in Chernozem and Luvisol soil contaminated with mercury. These results may indicate that the use of organic amendments such as digestate considerably improved the soil health in Chernozem and Luvisol compared with fly ash, alleviating the detrimental impact of Hg. Probably, the chemical properties present in digestate may determine its use as a suitable amendment for the assisted-natural attenuation of mercury-polluted soils.

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Changes in soil microbial community functionality and structure in a metal-polluted site: The effect of digestate and fly ash applications

Cited by 51 publications

References 64 publications

Effects of Pulp and Na-Bentonite Amendments on the Mobility of Trace Elements, Soil Enzymes Activity and Microbial Parameters under Ex Situ Aided Phytostabilization

Effects of Pulp and Na-Bentonite Amendments on the Mobility of Trace Elements, Soil Enzymes Activity and Microbial Parameters under Ex Situ Aided Phytostabilization

Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

Organic and inorganic amendment application on mercury-polluted soils: effects on soil chemical and biochemical properties

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