Changes in Serum Levels of Creatol and Methylguanidine in Renal Injury Induced by Lipid Peroxide Produced by Vitamin E Deficiency and GSH Depletion in Rats

Ozasa, Hisashi; Watanabe, Tomoe; Nakamura, Ko; Fukunaga, Yukihiro; Ienaga, Kazuharu; Hagiwara, Kiyokazu

doi:10.1159/000189536

Cited by 11 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found no age effect on the U-GAA/crn ratio in this cohort. Studies on guanidine compounds in rats (22,23) have demonstrated lower serum GAA levels in renal injury. This result might be explained by reduced AGAT activity, the enzyme that catalyzes conversion of glycine and arginine to ornithine and GAA (24).…”

Section: Discussionmentioning

confidence: 99%

Renal Function Influences Diagnostic Markers in Serum and Urine: A Study of Guanidinoacetate, Creatine, Human Epididymis Protein 4, and Neutrophil Gelatinase–Associated Lipocalin in Children

Salvador

Tøndel

Rowe

et al. 2017

The Journal of Applied Laboratory Medicine

View full text Add to dashboard Cite

Background: Impaired renal function may affect the level of diagnostic disease markers. The aim of the study was to investigate the effect of measured glomerular filtration rate (GFR) on 4 diagnostic markers in blood and urineguanidinoacetate (GAA), creatine (CRE), human epididymis protein 4 (HE4), and neutrophil gelatinase-associated lipocalin (NGAL)-and how this could affect the decision and reference limits. Methods: We examined 96 children (median age 9.2 years, range 0.25-17.5) with different stages of chronic kidney disease (CKD). GFR [median 65.9 mL • min −1 • (1.73 m 2) −1 , range 6.3-153] was measured by iohexol clearance using 7 venous blood samples after iohexol injection. Fasting serum and urinary GAA, CRE, HE4, NGAL, and creatinine (crn) were analyzed. After appropriate transformation of the markers, a multiple linear regression analysis examined the influence of age, sex, and measured GFR. Results: The level of GFR significantly affected S-GAA (P = 2 × 10 −4) and U-GAA/crn (P = 5 ×10 −11), leading to decreased values in renal impairment. GFR did not correlate significantly with the level of CRE and to a minor degree did the U-CRE/crn ratio (P = 0.54 and 0.01, respectively). The level of GFR significantly affected S-HE4 (P = 4 × 10 −31) and U-HE4/S-HE4 ratio (P = 2 × 10 −21) with increased serum values and decreased U-HE4/S-HE4 ratio in renal impairment. S-NGAL increased with decreasing kidney function (P = 2 × 10 −19). Conclusions: Diagnostic disease markers may be influenced by the renal function, and this must be taken into account when interpreting test results. Decreased renal function could change the level of the marker above or below decision limits, leading to diagnostic misinterpretation.

show abstract

Section: Discussionmentioning

confidence: 99%

Renal Function Influences Diagnostic Markers in Serum and Urine: A Study of Guanidinoacetate, Creatine, Human Epididymis Protein 4, and Neutrophil Gelatinase–Associated Lipocalin in Children

Salvador

Tøndel

Rowe

et al. 2017

The Journal of Applied Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Accordingly, the urinary excretion of guanidinoacetate is decreased in a variety of renal diseases (10,77,412,598,969). Although the serum concentration of guanidinoacetate was also shown to be decreased in both uremic patients and renal failure rats (39,412,520,598,747), it was found, in a few other studies, to be unchanged (10,165,168,638) or even slightly increased (163,757). These conflicting results may be due to compensatory upregulation of guanidinoacetate synthesis in the pancreas, to different degrees of depression of urinary guanidinoacetate excretion, to unknown effects of peritoneal or hemodialysis, and/or to different stages of disease progression.…”

Section: H Creatin(in)e Metabolism and Renal Diseasementioning

confidence: 99%

Creatine and Creatinine Metabolism

Wyss

Kaddurah‐Daouk

2000

Physiological Reviews

2,369

2,103

View full text Add to dashboard Cite

The goal of this review is to present a comprehensive survey of the many intriguing facets of creatine (Cr) and creatinine metabolism, encompassing the pathways and regulation of Cr biosynthesis and degradation, species and tissue distribution of the enzymes and metabolites involved, and of the inherent implications for physiology and human pathology. Very recently, a series of new discoveries have been made that are bound to have distinguished implications for bioenergetics, physiology, human pathology, and clinical diagnosis and that suggest that deregulation of the creatine kinase (CK) system is associated with a variety of diseases. Disturbances of the CK system have been observed in muscle, brain, cardiac, and renal diseases as well as in cancer. On the other hand, Cr and Cr analogs such as cyclocreatine were found to have antitumor, antiviral, and antidiabetic effects and to protect tissues from hypoxic, ischemic, neurodegenerative, or muscle damage. Oral Cr ingestion is used in sports as an ergogenic aid, and some data suggest that Cr and creatinine may be precursors of food mutagens and uremic toxins. These findings are discussed in depth, the interrelationships are outlined, and all is put into a broader context to provide a more detailed understanding of the biological functions of Cr and of the CK system.

show abstract

“…Serum levels of MG have been investigated since Giovanetti's group reported that MG accumulates in the serum of uremic patients as a uremic toxin. [14] Ozasa et al [15] reported that MG and CTL are present at similar levels in the serum of uremic rats. In humans, however, conversion of CTL to MG is poor, and MG can be detected as a minor Cr metabolite derived by reactive oxygen species.…”

Section: Discussionmentioning

confidence: 98%

Urinary Excretion of Creatol, an In Vivo Biomarker of Hydroxyl Radical, in Patients with Chronic Renal Failure

et al. 2007

Self Cite

View full text Add to dashboard Cite

Creatol (CTL) is a hydroxyl radical adduct of creatinine (Cr). The serum methylguanidine (MG) level and the MG/Cr molar ratio are reported to be biomarkers for oxidative stress. The aim of this study was to examine whether urinary excretion of CTL, another oxidative stress-related marker, is increased in patients with chronic renal failure (CRF). One hundred twentyfour non-dialyzed patients with chronic renal failure (serum Cr level, 1.3-10.0 mg/dL) were recruited from our hospitals. Urine and serum levels of CTL and MG were determined by high-performance liquid chromatography with the use of 9, 10-phenanthrenequinone as a fluorogenic reagent. The CTL/Cr and (CTL+MG)/Cr molar ratios in spot urine samples were also compared with those in 24-h urine samples. The urinary CTL/Cr and (CTL+MG)/Cr molar ratios increased with decreases in Cr clearance in patients with CRF. Correlations between serum and spot urine (CTL+MG)/Cr and between serum and spot urine CTL/Cr were quite similar to those in 24-h urine samples. CTL/Cr and (CTL+MG)/Cr molar ratios in both 24-h urine and spot urine samples appear to be useful indices of the severity of CRF.

show abstract

Changes in Serum Levels of Creatol and Methylguanidine in Renal Injury Induced by Lipid Peroxide Produced by Vitamin E Deficiency and GSH Depletion in Rats

Cited by 11 publications

References 18 publications

Renal Function Influences Diagnostic Markers in Serum and Urine: A Study of Guanidinoacetate, Creatine, Human Epididymis Protein 4, and Neutrophil Gelatinase–Associated Lipocalin in Children

Renal Function Influences Diagnostic Markers in Serum and Urine: A Study of Guanidinoacetate, Creatine, Human Epididymis Protein 4, and Neutrophil Gelatinase–Associated Lipocalin in Children

Creatine and Creatinine Metabolism

Urinary Excretion of Creatol, an In Vivo Biomarker of Hydroxyl Radical, in Patients with Chronic Renal Failure

Contact Info

Product

Resources

About