Changes in rumen fermentation and bacterial community in lactating dairy cows with subacute rumen acidosis following rumen content transplantation

Mu, Yingyu; Qi, Weibiao; Zhang, T.; Zhang, J.Y.; Mei, Shihui; Mao, Shengyong

doi:10.3168/jds.2021-20490

Cited by 24 publications

(17 citation statements)

References 50 publications

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“…The sequence analysis of the reads from the four treatments showed the phylum Bacteroidetes was the dominant bacteria, which was mainly composed of the genus Prevotella_1. Many reports suggested that the Prevotella genus was the dominant ruminal genus in the abundance of dairy cows (He et al, 2018;Mu et al, 2021), which was similar to the results of ours. Prevotella, which can degrade starch and protein, had many OTUs strongly associated with feed efficiency (Fregulia et al, 2021).…”

Section: Discussionsupporting

confidence: 91%

Calcium Propionate Supplementation Has Minor Effects on Major Ruminal Bacterial Community Composition of Early Lactation Dairy Cows

Zhang

Wang

et al. 2022

Front. Microbiol.

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Calcium propionate is one kind of good source for preventing and treating hypocalcemia and ketosis for dairy cows in early lactation. However, little is known about the effects of different feeding levels of calcium propionate on the ruminal bacterial community of early lactation dairy cows. This study aimed to explore the effects of different calcium propionate feeding levels on the ruminal fermentation and bacterial community composition of early lactation dairy cows. Twenty-four multiparous cows were randomly allocated into control (CON), low calcium propionate (LCaP), medium calcium propionate (MCaP), and high calcium propionate (HCaP) groups with six cows per group after calving. The CON group cows were fed the normal total mixed ration (TMR), and the cows of the LCaP, MCaP, and HCaP groups were fed TMR supplemented with 200, 350, and 500 g/day calcium propionate for 35 days after calving, respectively. The rumen fermentation parameters were measured every week, and the ruminal bacterial community composition of the last week was analyzed by 16S rRNA gene sequencing. Under the same diet, the rumen pH showed no difference among the four groups, but the content of microbial crude protein (MCP) and ammonia nitrogen quadratically decreased and linearly increased with calcium propionate supplementation, respectively. The feeding of calcium propionate linearly increased the concentrations of total volatile fatty acid (VFA), acetate, propionate, butyrate, iso-valerate, and valerate in the rumen. In all the treatment groups, Bacteroidetes, Firmicutes, and Proteobacteria were the dominant phyla, and Prevotella_1 and Succiniclasticum were the dominant genera in the rumen. Compared with the CON group, the addition of calcium propionate to the early lactation dairy cows quadratically improved the alpha diversity index of Chao1 estimator and observed species, but had little effect on the relative abundance of the major bacterial at phyla and genera level. These results suggested different levels of calcium propionate supplementation improved the rumen fermentation and the ruminal bacterial diversity but had little impact on the major ruminal bacterial community composition of dairy cows in early lactation.

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Section: Discussionsupporting

confidence: 91%

Calcium Propionate Supplementation Has Minor Effects on Major Ruminal Bacterial Community Composition of Early Lactation Dairy Cows

Zhang

Wang

et al. 2022

Front. Microbiol.

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“…Manipulating the fecal microbiota is a promising approach for changing metabolism ( Depommier et al, 2019 ; Gu et al, 2019 ), rebuilding microbial function ( Ridaura et al, 2013 ), and improving farm animals’ traits ( Li et al, 2019 ). Previous work in ruminants demonstrated that rumen fluid transplanting altered the rumen bacterial community ( Mu et al, 2021 ; Yin et al, 2021 ) and ruminal fermentation ( Liu et al, 2019 ). However, we still poorly understood what happened in the fecal microbiota and metabolism of cows following RFT.…”

Section: Discussionmentioning

confidence: 99%

“…In studies of humans and mice, fecal microbiota transplantation has been verified to be effective in reshaping the gut microbiota ( Dodiya et al, 2019 ; Tian et al, 2020 ), repairing the intestinal barrier ( Fischer et al, 2015 ), restoring intestinal function ( De Palma et al, 2017 ), and changing behavior ( Fujii et al, 2019 ; Hazan, 2020 ). In ruminants, rumen fluid or content transplanting was carried out to reshape the rumen microbiome and ruminal fermentation parameters, alleviate rumen epithelium damage ( Liu et al, 2019 ), as well as recover the rumen homeostasis ( Liu et al, 2019 ; Mu et al, 2021 ). In addition, Yu et al (2020) revealed that rumen fluid oral inoculation affected the rumen microbiota and the colonic microbiota, and increased the relative abundance of Veillonella , Ruminococcus 1, Anaerofilum , and Coprobacter in weaning lambs.…”

Section: Introductionmentioning

confidence: 99%

The Response of Fecal Microbiota and Host Metabolome in Dairy Cows Following Rumen Fluid Transplantation

et al. 2022

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Rumen fluid transplantation (RFT) has been used to rebuild rumen bacterial homeostasis, reshape rumen function, and restore rumen fermentation, whereas the effect of RFT on fecal microbiota and host metabolism in cows remains poorly understood. In our study, a combination of 16S rRNA sequencing and serum non-targeted metabolomics was performed to investigate the response of fecal microbiota and serum metabolome in dairy cows following RFT. Twenty-four prepartum dairy cows were randomly assigned to 3 groups (n = 8) for infusion of either saline (Con), fresh rumen fluid (FR), or sterilized rumen fluid (SR) after calving. Fourteen days after calving, fecal microbiota and serum metabolome were analyzed. The sequencing data of fecal samples revealed no changes in alpha diversity and relative abundance of dominant genera such as Ruminococcaceae UCG-005, Rikenellaceae RC9 gut and Eubacterium coprostanoligenes. However, the other genus level taxa, such as Eubacterium oxidoreducens, Anaerorhabdus furcosa, Bacillus and Selenomonas, showed distinct changes following RFT. Serum metabolome analysis showed that FR or SR infusion affected amino acids metabolism, bile acids metabolism and fatty acids metabolism (including linoleic acid, oleic acid and palmitic acid). Furthermore, correlation analysis showed that taxa from genera Clostridiales were positively correlated with metabolites involved in tryptophan and bile acid metabolisms, such as OTU1039 from genera unclassified o_Clostridiales was positively correlated to indoleacetic acid and taurolithocholic acid. These results suggest that RFT altered the composition of the fecal microbiota and modulated microbial metabolic pathways, which is vital for the development and safety assessment of rumen microbial intervention strategies.

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“…Disturbance in the rumen microbiota is associated with the development of various diseases. Recent studies showed that alterations in rumen microbiota and metabolite activity were observed in dairy cattle and goats with SARA [ 65 , 66 ]. The richness and diversity of the rumen and fecal microbiota were reduced in grain-based SARA of Danish Holstein cows [ 67 ].…”

Section: The Mechanism Of Sara-mediated Igl and Related Inflammatory ...mentioning

confidence: 99%

“…In addition, rumen transplantation is commonly used in the treatment of digestive disorders, including left-sided abomasal displacement [ 120 ]. Recently, evidence also suggested that rumen content transplantation (RCT) can return to normal levels with rumen fermentation parameters, including the levels of bacterial community diversity and the concentrations of acetate, valerate and VFA, in SARA cows induced by a high-grain diet [ 65 ]. Furthermore, rumen fluid transplantation (RT) also changed the gastrointestinal microbiota and then influenced the feed intake, feed digestibility and growth performance of weaned lambs [ 121 ].…”

Section: Regulating the Rumen Microbiota To Prevent Sara And Related ...mentioning

confidence: 99%

The Role of Rumen Microbiota and Its Metabolites in Subacute Ruminal Acidosis (SARA)-Induced Inflammatory Diseases of Ruminants

Xiang

et al. 2022

Microorganisms

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Subacute ruminal acidosis (SARA) is a common metabolic disease in ruminants. In the early stage of SARA, ruminants do not exhibit obvious clinical symptoms. However, SARA often leads to local inflammatory diseases such as laminitis, mastitis, endometritis and hepatitis. The mechanism by which SARA leads to inflammatory diseases is largely unknown. The gut microbiota is the totality of bacteria, viruses and fungi inhabiting the gastrointestinal tract. Studies have found that the gut microbiota is not only crucial to gastrointestinal health but also involved in a variety of disease processes, including metabolic diseases, autoimmune diseases, tumors and inflammatory diseases. Studies have shown that intestinal bacteria and their metabolites can migrate to extraintestinal distal organs, such as the lung, liver and brain, through endogenous pathways, leading to related diseases. Combined with the literature, we believe that the dysbiosis of the rumen microbiota, the destruction of the rumen barrier and the dysbiosis of liver function in the pathogenesis of SARA lead to the entry of rumen bacteria and/or metabolites into the body through blood or lymphatic circulation and place the body in the “chronic low-grade” inflammatory state. Meanwhile, rumen bacteria and/or their metabolites can also migrate to the mammary gland, uterus and other organs, leading to the occurrence of related inflammatory diseases. The aim of this review is to describe the mechanism by which SARA causes inflammatory diseases to obtain a more comprehensive and profound understanding of SARA and its related inflammatory diseases. Meanwhile, it is also of great significance for the joint prevention and control of diseases.

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Changes in rumen fermentation and bacterial community in lactating dairy cows with subacute rumen acidosis following rumen content transplantation

Cited by 24 publications

References 50 publications

Calcium Propionate Supplementation Has Minor Effects on Major Ruminal Bacterial Community Composition of Early Lactation Dairy Cows

Calcium Propionate Supplementation Has Minor Effects on Major Ruminal Bacterial Community Composition of Early Lactation Dairy Cows

The Response of Fecal Microbiota and Host Metabolome in Dairy Cows Following Rumen Fluid Transplantation

The Role of Rumen Microbiota and Its Metabolites in Subacute Ruminal Acidosis (SARA)-Induced Inflammatory Diseases of Ruminants

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