Changes in related circular RNAs following ERβ knockdown and the relationship to rBMSC osteogenesis

Li, Xiaoyun; Peng, Bojia; Zhu, Xiaofeng; Wang, Panpan; Xiong, Yingquan; Liu, Hengrui; Sun, Kai; Wang, Haixia; Ou, Ling; Wu, Zhidi; Liu, Xiaoguang; He, Hua; Mo, Shu; Peng, Xunqian; Tian, Ya‐Chung; Zhang, Ronghua; Yang, Li

doi:10.1016/j.bbrc.2017.09.068

Cited by 48 publications

(32 citation statements)

References 23 publications

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“…The snoRNA Snord116 is closely related to the bone-mass phenotype in people with Prader–Willi syndrome [ 9 ]. Circular RNAs (circRNAs) may play important roles in bone formation [ 10 ], and the circRNA–miRNA–mRNA network may function in osteogenesis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Bone remodeling induced by mechanical forces is regulated by miRNAs

et al. 2018

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The relationship between mechanical force and alveolar bone remodeling is an important issue in orthodontics because tooth movement is dependent on the response of bone tissue to the mechanical force induced by the appliances used. Mechanical cyclical stretch (MCS), fluid shear stress (FSS), compression, and microgravity play different roles in the cell differentiation and proliferation involved in bone remodeling. However, the underlying mechanisms are unclear, particularly the molecular pathways regulated by non-coding RNAs (ncRNAs) that play essential roles in bone remodeling. Amongst the various ncRNAs, miRNAs act as post-transcriptional regulators that inhibit the expression of their target genes. miRNAs are considered key regulators of many biologic processes including bone remodeling. Here, we review the role of miRNAs in mechanical force-induced bone metabolism.

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Section: Introductionmentioning

confidence: 99%

Bone remodeling induced by mechanical forces is regulated by miRNAs

et al. 2018

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“…There are few reports on the physiological processes involving circRNAs in MSCs. Li et al reported that estrogen receptor β regulates osteogenesis of MSCs through the 2:27713879/27755789/2:240822115/240867796‐miR‐328‐5p‐mRNA axis. CircRNAs and their interacting miRNAs play an important role in the osteogenic differentiation of BMSCs .…”

Section: Discussionmentioning

confidence: 99%

“…Maass et al reported expression of distinct and specific circRNAs in MSCs as well as in chondrocytes, osteoblasts, and vascular smooth muscle cells differentiated from MSCs. Li et al revealed that estrogen receptor β regulates osteogenic differentiation of MSCs through the 2:27713879/27755789/2:240822115/240867796‐miR‐328‐5p‐mRNA axis. Zhang et al uncovered numerous circRNAs differentially expressed during BMSC osteogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Altered expression of circular RNAs in human placental chorionic plate‐derived mesenchymal stem cells pretreated with hypoxia

Sun

Jin

Liang

et al. 2018

Clinical Laboratory Analysis

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Background Hypoxic preconditioning alters the biological properties of mesenchymal stem cells (MSCs). It is not known whether this process has an effect on circular RNAs (circRNAs) in MSCs. Methods Human placental chorionic plate‐derived MSCs (hpcpMSCs) isolated from the same placentae were classed into two groups: hypoxic pretreated (hypoxia) group and normally cultured (normoxia) group. The comparative circRNA microarray analysis was used to determine circRNAs expression and verified by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) in the two groups. Results One hundred and two differentially expressed circRNAs in the hypoxia group were found compared to that in the normoxia group (fold change >1.5‐fold and P < 0.05). The expression levels of circRNAs by qRT‐PCR were consistent with those evaluated by microarray analysis. Gene ontology (GO) analysis showed that the putative function of their target genes for those differentially expressed circRNAs was primarily involved in cell development and its differentiation and regulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that transcriptional misregulation in cancer and mitogen‐activated protein kinase (MAPK) signaling pathway were the most significant. MAPK signaling pathway was found to be the core regulatory pathway triggered by hypoxia. Conclusions The results indicate that the altered expression of specific circRNAs in MSCs is associated with hypoxic preconditioning. This finding provides further exploration of underlying mechanisms of the characteristic changes of MSCs with hypoxic preconditioning.

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“…All raw data in control and CM group was processed with ‘percentile’ method for data normalization, and FC (fold-change) was calculated using these normalized datas. The miRNAs with FC ≥2.0 and P<0.05 were considered significant, the choice of cut-off was based on previous studies ( 15 – 18 ). This section was accomplished by Beijing CapitalBio Corporation (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Specific miRNA expression profile in the blood serum of cardiac myxoma patients

Liu

2018

Oncol Lett

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The profile of differentially expressed microRNAs (miRNAs) in the serum of patients with cardiac myxoma (CM) (n=30) and healthy people (n=30) was studied using miRNA microarray analysis. The expression of the candidate miRNAs was validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in the serum of 30 CM patients and 30 healthy control individuals. TargetScan, PicTar and miRanda were used to predict the possible target gene of miR-320a. The Gene Ontology database and Kyoto Encyclopedia of Genes and Genomes database were used to enrich the functions and signaling pathways of the target genes, respectively. The results showed that 4 differentially expressed miRNAs were identified, the expression levels of miR-320a and miR-1249-5p were upregulated, and those of miR-634 and miR-6870-3p were downregulated in CM patients (P<0.05). The expression levels of miR-320a and miR-634 selected for verification by RT-qPCR were in high concordance with the results of microarray analysis. Through bioinformatics, we identified 487 target genes predicted from miR-320a, that were mostly enriched in the bone morphogenetic protein signaling pathway, nicotinamide adenine dinucleotide pathway and de novo ceramide biosynthetic pathway. In our study, we reported for the first time the circulating miRNA profile of CM patients and suggested that miR-320a may participate in CM development through the ceramide signaling pathway.

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Changes in related circular RNAs following ERβ knockdown and the relationship to rBMSC osteogenesis

Cited by 48 publications

References 23 publications

Bone remodeling induced by mechanical forces is regulated by miRNAs

Bone remodeling induced by mechanical forces is regulated by miRNAs

Altered expression of circular RNAs in human placental chorionic plate‐derived mesenchymal stem cells pretreated with hypoxia

Specific miRNA expression profile in the blood serum of cardiac myxoma patients

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