Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach

Muñoz-Prieto, Alberto; Contreras-Aguilar, María Dolores; Cerón, José J.; Ayala, Ignacio; Martín‐Cuervo, María; González-Sánchez, Juan Carlos; Jacobsen, Stine; Kuleš, Josipa; Beletić, Anđelo; Rubić, Ivana; Mrljak, Vladimir; Tecles, Fernando; Hansen, Sanni

doi:10.3390/ani12091169

Cited by 12 publications

(8 citation statements)

References 40 publications

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“…Serum and saliva showed different responses in our experimental conditions, with differences in the number of proteins showing significant changes as well as in the pattern of proteins with variations. These discrepancies in the number and types of proteins that change between these fluids have been observed previously in other species and diseases [ 40 , 41 , 42 , 43 ]. Therefore, saliva and serum could present complementary information when analyzed.…”

Section: Discussionsupporting

confidence: 51%

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

López-Martínez

Cerón

Ortín-Bustillo

et al. 2022

IJMS

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Sepsis is a systemic inflammatory response triggered by an infectious agent and is recognized by the World Health Organization as a global concern, since it is one of the major causes of severe illness in humans and animals. The study of the changes that can occur in saliva and serum in sepsis can contribute to a better understanding of the pathophysiological mechanisms involved in the process and also to discover potential biomarkers that can help in its diagnosis and monitoring. The objective of this study was to characterize the changes that occur in the salivary and serum proteome of pigs with experimentally-induced sepsis. The study included five pigs with sepsis induced by LPS administration and five pigs with non-septic inflammation induced by turpentine for comparative purposes. In saliva, there were eighteen salivary proteins differentially expressed in the sepsis condition and nine in non-septic inflammation. Among these, significant increments in aldolase A and serpin B12 only occurred in the sepsis model. Changes in aldolase A were validated in a larger population of pigs with sepsis due to Streptococcus suis infection. In serum, there were 30 proteins differentially expressed in sepsis group and 26 proteins in the non-septic group, and most of the proteins that changed in both groups were related to non-specific inflammation. In the saliva of the septic animals there were some specific pathways activated, such as the organonitrogen compound metabolic process and lipid transport, whereas, in the serum, one of the main activated pathways was the regulation of protein secretion. Overall, saliva and serum showed different proteome variations in response to septic inflammation and could provide complementary information about the pathophysiological mechanisms occurring in this condition. Additionally, salivary aldolase A could be a potential biomarker of sepsis in pigs that should be confirmed in a larger population.

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Section: Discussionsupporting

confidence: 51%

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

López-Martínez

Cerón

Ortín-Bustillo

et al. 2022

IJMS

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“…One study even showed a correlation between histopathological gastric glandular inflammation to duodenal inflammation, including lymphoplasmacytic inflammation and eosinophilic infiltrate, but not to more distal segments of the GI tract [ 67 ]. This evidence of EGGD as a form of gastritis is supported by evidence of immune upregulation in EGGD, demonstrated by altered protein composition of serum and saliva of horses with EGGD compared to controls [ 89 ]. Further work is needed to deepen the understanding of inflammation in EGGD pathophysiology.…”

Section: Pathophysiologymentioning

confidence: 97%

Equine Gastric Ulcer Syndrome: An Update on Current Knowledge

Vokes

Lovett

Sykes

2023

Animals

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Equine Gastric Ulcer Syndrome (EGUS) is a term that has been used since 1999, initially being used to describe all gastric mucosal disease in horses. Since this time, the identification of two distinct main disease entities of the equine gastric mucosa have been described under the umbrella of EGUS; these are Equine Squamous Gastric Disease (ESGD) and Equine Glandular Gastric Disease (EGGD). In 2015 the European College of Equine Internal Medicine (ECEIM) released a consensus statement defining these disease entities. This document highlighted the lack of evidence surrounding EGGD compared to ESGD, and identified knowledge gaps for further research to be directed. Subsequently, many studies on EGGD have been published, especially on pathophysiology, diagnosis, and treatment. This article updates current knowledge on both ESGD and EGGD as understanding has evolved since the last large-scale review.

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“…In addition, other biomarkers related to oxidative stress, such as ferric-reducing activity of saliva (FRAS), uric acid (UA), and the advanced oxidation protein products (AOPP) were increased in the saliva of horses with EGGD compared with healthy animals, whereas no significant increase was observed in cases of ESGD [ 8 ]. Moreover, a proteomic approach comparing saliva of horses with ESGD and EGGD showed that proteins related with epithelial regulation, such as serpin B5 and keratins 15 (KRT15) and 4 (KRT4), were upregulated in ESGD compared to EGGD [ 9 ]. Additionally, other salivary biomarkers have been reported to be potentially useful in horses with acute abdominal disease [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome

Muñoz-Prieto

Cerón

Rubio

et al. 2022

Animals

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In this report, the measurement of salivary biomarkers as an aid for diagnosis of equine gastric ulcer syndrome (EGUS) was studied. A comprehensive panel of 23 salivary analytes was measured in the saliva of horses affected by EGUS and compared to healthy animals and horses with other diseases clinically similar to EGUS but with a negative diagnosis at gastroscopic examination. A total of 147 horses were included in the study and divided into heathy population (n = 12), the EGUS group (n = 110), and the group of horses with other diseases (n = 25). From the 23 analytes studied, 17 showed increased values in EGUS horses when compared to healthy ones, and uric acid, triglycerides, and calcium were significantly increased in horses with EGUS compared to the group of other diseases. The receiver operating characteristic curve analyses showed a modest but significant discriminatory power of those three analytes to identify EGUS from other diseases with similar symptoms. The discriminatory power enhanced when the results of the three analytes were combined. In conclusion, the results showed that selected salivary analytes could have potential use as biomarkers in horses with EGUS.

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Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach

Cited by 12 publications

References 40 publications

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

A Proteomic Approach to Elucidate the Changes in Saliva and Serum Proteins of Pigs with Septic and Non-Septic Inflammation

Equine Gastric Ulcer Syndrome: An Update on Current Knowledge

Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome

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