Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis

Abstract

Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have investigated their effects at a proteome level. Herein, we characterise the protein expression changes that occur in lamina propria (LP) and the intestinal epithelial cells (IEC) of mice with dextran sulfate sodium (DSS)-induced colitis treated with Ancylostoma caninum excretory/secretory (ES) products using a quantitative proteomic approach. We have shown how parasite products can significantly alter the expression of proteins involved in immune responses, cell death and with an antioxidant activity. Interestingly, significant changes in the expression levels of different mucins were observed in this study. MUC13, a mucin implicated in gastrointestinal homeostasis, was upregulated in the LP of mice with DSS-induced colitis treated with ES, while MUC2, a major component of mucus, was upregulated in the IEC. In addition, A. caninum proteins have an important effect on proteins with antioxidant functions and proteins involved in intestinal homeostasis and tissue integrity and regeneration. Understanding how parasites can ameliorate IBD pathogenesis can help us design novel treatments for autoimmune diseases.

Revisiting the Ancylostoma Caninum Secretome Provides New Information on Hookworm–Host Interactions

“…The ESPs contain proteins that facilitate a parasitic existence, notably penetration of and migration within a host, feeding on host tissues, and evasion of the host immune response . In addition, recently, hookworm ESPs have been shown to contain immunoregulatory properties that can protect mice against inflammatory diseases such as inflammatory bowel diseases and asthma …”

Exosomes Derived from Dendritic Cells Treated with Schistosoma japonicum Soluble Egg Antigen Attenuate DSS-Induced Colitis

“…Furthermore, Schistosoma mansoni and Ancylostoma caninum soluble proteins hold therapeutic potential to treat 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice ( Ruyssers et al, 2009 ). A. caninum excretory/secretory products can ameliorate the pathogenesis of colitis in mice ( Sotillo et al, 2017 ), and Schistosoma japonicum and S. mansoni eggs have modulatory effects on colitis in mice ( Yang et al, 2007 ; Xia et al, 2011 ; Hasby et al, 2015 ). Therefore, parasite therapy has been suggested; however, this treatment has potentially harmful effects.…”

The excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis induces autophagy via the Sonic hedgehog pathway in mouse brain astrocytes

“…During parasitic helminth infection, parasites, including Angiostrongylus cantonensis, Ancylostoma caninum, hookworm, Trichinella spiralis, Trichuris suis, Haemonchus contortus, Anisakis pegreffii and Contracaecum osculatum, can produce excretory-secretory products (ESPs) to modulate the host response [33,[37][38][39][40][41][42][43]. ESPs play an important role in the interaction between parasites and hosts and are essential factors for host tissue invasion, digestion, feeding, development, molting, host immune responses, oxidative stress, and apoptosis [40,44].…”