Changes in properties of phytopathogenic bacteria effected by plasmid pRD1

Kozyrovska, Natalia; Gvozdyak, R. I.; Muras, V. A.; Kordyum, V. A.

doi:10.1007/bf00410731

Cited by 17 publications

(11 citation statements)

References 9 publications

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“…The loss of pathogenic phenotype has been reported upon the transfer of pRD1 from E. coli to E. carotovora [23]. The transconjugants of E. carotovora obtained in the present study showed no such loss of pathogenicity and retained the ability to cause maceration of potato tuber tissue, thus indicating that Inc-P plasmids do not affect the pathogenic phenotype of E. carotovora.…”

Section: Carotovorasupporting

confidence: 53%

Studies on Inc-P plasmids inErwinia carotovorasubsp.carotovora

Jayaswal

Bressan

Charles

et al. 1986

FEMS Microbiology Letters

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Inc‐P plasmids, RP4, R751, pMO850, and pRK2013 were transferred to Erwinia carotovora. These plasmids were stably maintained in E. carotovora and the transconjugants were efficient donors of respective plasmids to other strains of E. carotovora and Escherichia coli. These plasmids were not able to mobilize chromosomal markers from one strain of E. carotovora to another strain of E. carotovora even in the presence of homologous DNA sequences on the plasmid and the bacterial chromosome. The presence of Inc‐P plasmid does not affect the pathogenic phenotype of E. carotovora. A broad host range Inc‐P cosmid, pLAFR1, was transferred to E. carotovora with the help of pRK2013, suggesting the potential use of a binary plasmid system for genetic complementation in E. carotovora.

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Section: Carotovorasupporting

confidence: 53%

Studies on Inc-P plasmids inErwinia carotovorasubsp.carotovora

Jayaswal

Bressan

Charles

et al. 1986

FEMS Microbiology Letters

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“…The presence of pectic substrates in the soil and the diminished gene/protein production resulting from the deletion of the pectate lyase gene may have contributed to the significantly lower survival of the genetically engineered strain in the loam soil. Such alterations in cell physiology and biochemistry have been reported with E. carotovora transconjugants [24]. With respect to the variable survival pattern of the GEM in the loam soil, Scanferlato and colleagues [15] show a similar variable recovery pattern of a genetically engineered Erwinia strain using MPN analysis.…”

Section: Discussionmentioning

confidence: 66%

Survival and ecological effects of genetically engineered Erwinia carotovora in soil and aquatic microcosms

Palmer

Scanferlato

Orvos

et al. 1997

Enviro Toxic and Chemistry

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The release of genetically engineered organisms into the environment has raised concerns about their potential ecological impact. In this study, genetically engineered Erwinia carotovora strains expressing varying levels of reduced phytopathogenicity and wildtype E. carotovora strains were used in aquatic and soil microcosms to assess the survival, intraspecific competition, and effects upon specific groups of indigenous bacteria. In aquatic microcosms, the densities of Erwinia genetically engineered organisms (GEMs) and wildtype strains declined and fell below the detectable limit of plate counts 15 d after the microcosms were inoculated. In aquatic microcosms, engineered E. carotovora did not exhibit a competitive advantage over the wildtype. The effect of engineered and wildtype E. carotovora on densities of total and selected bacteria was not significantly different. Treatment with engineered E. carotovora did not change biomass values of the receiving community but did cause a transitory increase in metabolic activity. In aquatic microcosms, the inability of genetically engineered E. carotovora to persist, displace resident species, or affect the metabolic activity of aquatic communities indicates the low risk of adverse ecological consequences in aquatic ecosystems. Unlike previous investigations involving soil microcosms, densities of both the genetically engineered and wildtype E. carotovora remained at detectable levels over 60 d in both agricultural clay and forest loam soils. The type of soil significantly affected the survival of the GEM and the wildtype. The sorptive properties of clay particles, as well as low concentrations of soil nutrients and organic matter, may have contributed to the unexpected patterns of GEM and wildtype survival.

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“…The presence of pectic substrates in the soil and the diminished gene/protein production resulting from the deletion of the pectate lyase gene may have contributed 0 2 5 10 15 20 25 30 67 72 85 74 85 103 102 99 9 10 8 7 1 26 11 32 81 84 87 78 103 100 89 117 13 13 25 17 25 18 17 21 to the significantly lower survival of the genetically engineered strain in the loam soil. Such alterations in cell physiology and biochemistry have been reported with E. carotovora transconjugants [24]. With respect to the variable survival pattern of the GEM in the loam soil, Scanferlato and colleagues [15] show a similar variable recovery pattern of a genetically engineered Erwinia strain using MPN analysis.…”

Section: Discussionmentioning

confidence: 67%

Survival and Ecological Effects of Genetically Engineered Erwinia Carotovora in Soil and Aquatic Microcosms

Palmer¹,

Scanferlato²,

Orvos³

et al. 1997

Environ Toxicol Chem

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Abstract-The release of genetically engineered organisms into the environment has raised concerns about their potential ecological impact. In this study, genetically engineered Erwinia carotovora strains expressing varying levels of reduced phytopathogenicity and wildtype E. carotovora strains were used in aquatic and soil microcosms to assess the survival, intraspecific competition, and effects upon specific groups of indigenous bacteria. In aquatic microcosms, the densities of Erwinia genetically engineered organisms (GEMs) and wildtype strains declined and fell below the detectable limit of plate counts 15 d after the microcosms were inoculated. In aquatic microcosms, engineered E. carotovora did not exhibit a competitive advantage over the wildtype. The effect of engineered and wildtype E. carotovora on densities of total and selected bacteria was not significantly different. Treatment with engineered E. carotovora did not change biomass values of the receiving community but did cause a transitory increase in metabolic activity. In aquatic microcosms, the inability of genetically engineered E. carotovora to persist, displace resident species, or affect the metabolic activity of aquatic communities indicates the low risk of adverse ecological consequences in aquatic ecosystems. Unlike previous investigations involving soil microcosms, densities of both the genetically engineered and wildtype E. carotovora remained at detectable levels over 60 d in both agricultural clay and forest loam soils. The type of soil significantly affected the survival of the GEM and the wildtype. The sorptive properties of clay particles, as well as low concentrations of soil nutrients and organic matter, may have contributed to the unexpected patterns of GEM and wildtype survival.

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Changes in properties of phytopathogenic bacteria effected by plasmid pRD1

Cited by 17 publications

References 9 publications

Studies on Inc-P plasmids inErwinia carotovorasubsp.carotovora

Studies on Inc-P plasmids inErwinia carotovorasubsp.carotovora

Survival and ecological effects of genetically engineered Erwinia carotovora in soil and aquatic microcosms

Survival and Ecological Effects of Genetically Engineered Erwinia Carotovora in Soil and Aquatic Microcosms

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