Changes in pools of autoantibodies and anti-bacterial antibodies in patients suffering from recurrent infections of the urinary tract and undergoing bacterial immunization treatment

Stojanovi, Miodrag

doi:10.1016/s0165-2478(04)00110-5

Cited by 3 publications

(4 citation statements)

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“…Titers of anti‐LMN Abs were elevated in patients with viral and parasitic infections. Serum Abs against LMN have been detected in patients with SSc, Raynaud's phenomena, and the MRL‐lpr‐mouse strain 16–18 . In a recent study, pathogenic murine lupus Abs reacted with a 21‐mer peptide located in the globular part of the alpha‐chain of LMN.…”

Section: Discussionmentioning

confidence: 99%

Autoantibodies in Nonautoimmune Individuals during Infections

Berlin

Zandman‐Goddard

Blank

et al. 2007

Annals of the New York Academy of Sciences

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Infections can act as environmental triggers inducing or promoting autoimmune disease in genetically predisposed individuals. Identification of microbial peptides similar to self-tissues may by molecular mimicry, provide the inducing mechanism for an immune response. The aim of this study was to identify autoantibodies (autoAbs) in nonautoimmune individuals during acute bacterial, viral, or parasitic infections. Specific Abs or specific infections with an increased autoAb load may shed insight into the mechanisms of autoimmune disease. Sera from 88 patients with acute infections (41 bacterial, 23 viral, 17 parasitic, and 7 rickettsial) were tested by the ELISA method for antinuclear antibodies (ANA) 8 Pro, and Abs to thyroid peroxidase (TPO), thyroglobulin, phospholipids, annexin-V, laminin, anti-Saccharomyces cervisiae (ASCA), and prothrombin, along with 80 normal controls. Elevated titers of Abs to annexin-V and prothrombin were the most prevalent in viral, parasitic, and rickettsial infections and to laminin in viral and parasitic infections. Elevated titers of ASCA and ANA were found in viral and bacterial infections. Antiphospholipid Abs were found in parasitic and Q-fever infections. Thirty-four individuals harbored elevated titers of at least two Abs. An autoAb burden was detected in individuals with hepatitis A, hepatitis B, toxoplasma or Q-fever infections. In nonautoimmune individuals with various (bacterial, viral, parasitic, and rickettsial) infections, elevated titers of Abs to annexin-V, prothrombin, laminin, ASCA, ANA, and phospholipids were most frequently detected.

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Section: Discussionmentioning

confidence: 99%

Autoantibodies in Nonautoimmune Individuals during Infections

Berlin

Zandman‐Goddard

Blank

et al. 2007

Annals of the New York Academy of Sciences

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“…Among the possible mechanisms that contribute to autoAb generation, the most cited mechanisms are molecular mimicry (9, 10) and polyclonal cell activation (11, 12). Available data show that long‐term stimulation by micAg is accompanied by augmentation of self‐reactivity, but not by obvious induction of autoimmune diseases (13). Hence, we have tried to create a model system for studying the connection between micAg, autoimmunity and autoimmune disease on the basis of hyper‐immunization and application of micAg in different contexts.…”

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confidence: 99%

The context of tetanus toxoid application influences the outcome of antigen‐specific and self‐directed humoral immune response

Stojanović

Živković

Inić-Kanada

et al. 2009

Microbiology and Immunology

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Results are presented concerning our attempts to create a suitable model system for studying the connection between microbial antigen (micAg), autoimmunity and autoimmune disease on the basis of hyper-immunization and application of micAg in different contexts. Our research was focused on tetanus toxoid (TTd) as a model micAg. Non-pretreated and complete Freund's adjuvant pretreated BALB/c mice were immunized with high doses of TTd mixed with glycerol or aluminum hydroxide as adjuvants. The main aims of the experiments were to evaluate the properties of induced humoral immune responses, evaluate the pathological potential of induced immune responses and determine possible correlations between the properties of a humoral immune response and its pathological potential. The production of TTd-specific and self-reactive β 2 -glycoprotein I (β 2 -GP I)-specific antibodies (Abs) was detected in all groups but with specific, context-related properties. Analysis of pregnancy-related pathology (anti-β 2 -GP I Abs-associated) showed differences in the pathological potential of the induced immune response. It was demonstrated that severity of pathology is positively correlated to the abundance of IgG that recognizes β 2 -GP I adsorbed onto phosphatidylserine, and to IgG affinity. Furthermore, it was demonstrated that molecular mimicry, which results in generation of anti-β 2 -GP I Abs upon TTd immunization, is necessary but not sufficient for the development of pregnancy-related pathology.Key words adjuvant, anti-β 2 -glycoprotein I antibody, tetanus toxoid.Various data that connect exposure to micAg, during infections (1-4) and vaccinations (5-8), to autoAb production have been published in recent years. Among the possible mechanisms that contribute to autoAb generation, the most cited mechanisms are molecular mimicry (9, 10) and polyclonal cell activation (11,12). Available data show that long-term stimulation by micAg is accompanied by augmentation of self-reactivity, but not by obvious induc- tion of autoimmune diseases (13). Hence, we have tried to create a model system for studying the connection between micAg, autoimmunity and autoimmune disease on the basis of hyper-immunization and application of micAg in different contexts.With the intention of creating a suitable model system, we focused our research on TTd, a chemical derivative of tetanus toxin, as a model antigen. As a single protein

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“…Patients were therapeutically vaccinated with a polyvalent mixture containing seven different strains of heat-inactivated bacteria (Stojanovic et al, 2004 (Pseudomonas aeruginosa) and Serratia sp. (Serratia marcescens).…”

Section: Patients Immunization and Bleeding Proceduresmentioning

confidence: 99%

“…In our previous work, we have described the case of female patients suffering from uncomplicated recurrent bacterial infections of the lower urinary tract (RBI) (Stojanovic et al, 2004). The patients were therapeutically vaccinated with the mixture of heat-inactivated bacteria (Prevalur ® ) of which Proteus sp., Klebsiella sp.…”

Section: Introductionmentioning

confidence: 99%

Changes in Composition of IgM Polymers in Patients Suffering from Recurrent Urinary Bacterial Infections after Bacterial Immunization Treatment

Petrušić

Stojanović

Živković

et al. 2010

Immunological Investigations

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IgM is the first antibody produced during the immune response to infection or immunization, and it can be secreted as pentamer (containing a small polypeptide, termed as J chain) or hexamer (lacking J chain). In this paper we have analyzed structural characteristics (by electrophoresis and immunoblot) and anti-bacterial specificity (by enzyme-linked immunosorbent assay) of IgM antibodies purified from female patients suffering from recurrent bacterial infections of lower urinary tract and therapeutically immunized with the mixture of heat-inactivated uropathogenic bacteria. We report on changes in the composition of IgM polymers in tested patients. The immunization induced the raise of the levels of hexamers in patients that did not respond to immunization (non-responders), while the IgM polymers remained on the pentameric level in immunization dependent responders. We propose that the composition of IgM polymers could influence the immunization outcome and should be taken in count regarding the treatment of recurrent bacterial infections.

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Changes in pools of autoantibodies and anti-bacterial antibodies in patients suffering from recurrent infections of the urinary tract and undergoing bacterial immunization treatment

Cited by 3 publications

References 0 publications

Autoantibodies in Nonautoimmune Individuals during Infections

Autoantibodies in Nonautoimmune Individuals during Infections

The context of tetanus toxoid application influences the outcome of antigen‐specific and self‐directed humoral immune response

Changes in Composition of IgM Polymers in Patients Suffering from Recurrent Urinary Bacterial Infections after Bacterial Immunization Treatment

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