Changes in polypeptides in Pharbitis cotyledons during the first flower-inductive photoperiod

Ono, Michiyuki; Ono, Kimiyo Sage; Yasui, Masaru; Okasaki, Mitsuo; Harada, Hiroshi

doi:10.1016/0168-9452(93)90121-f

Cited by 12 publications

(9 citation statements)

References 17 publications

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“…These physiological characteristics make P. nil an attractive model plant for research on flowering (Vince-Prue and Gressel, 1985). In several studies at the molecular level, attempts have been made to identify genes associated with flowering (Lay-Yee et al, 1987;Ono et al, 1993Ono et al, , 1996Zheng et al, 1993Zheng et al, , 1998O'Neill et al, 1994;Sage-Ono et al, 1998). However, there is no conclusive evidence that any of these genes play a role in flower initiation.…”

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confidence: 99%

Isolation of a CONSTANS Ortholog from Pharbitis nil and Its Role in Flowering

Liu¹,

Yu²,

McIntosh³

et al. 2001

Plant Physiology

114

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The short-day plant Pharbitis nil is a model plant for the study of photoperiodic control of floral initiation. Flower formation can be induced at the cotyledon stage by a single long night of at least 14 h in duration. Using differential display of mRNA we identified a P. nil ortholog of the ArabidopsisCONSTANS (CO) gene, which will be referred to as PnCO. Expression of PnCOwas high after a 14-h night, but low when the dark period was 12 h or less. Our results indicate that the level of the PnCOtranscript is photoperiodically regulated. After transfer from continuous light to darkness, PnCO showed a circadian pattern of expression. Expression of the CAB gene, which is a molecular marker for the circadian clock, exhibited a different pattern of expression than did PnCO and was not subject to the same photoperiodic control. A major portion of thePnCO transcripts contained an unspliced intron. Only the intron-free PnCO was able to complement theco mutant of Arabidopsis by shortening the time to flower.

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confidence: 99%

Isolation of a CONSTANS Ortholog from Pharbitis nil and Its Role in Flowering

Liu¹,

Yu²,

McIntosh³

et al. 2001

Plant Physiology

114

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“…Qualitative changes in the protein complement of induced organs leaves or cotyledons have been reported for Xanthium strumarium (Kannangara et al 1990), but have not been found among other short day (SD) plants (SDPs), such as Pharbitis nil (Ono et al 1993), Impatiens balsamina (Sawhney et al 1976) and Amaranthus caudatus (Ballard and Jenkins 1991), or among long day (LD) plants (LDPs) such as Spinacia oleracea (Lefort et al 1991). On the other hand, quantitative changes have been found and may be subtle since some were not observed in two-dimensional (2D) gels after regular staining, but only after in-vivo labelling and gel-autoradiography (Lefort et al 1991;Ono et al 1993).…”

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confidence: 96%

Changes in gene expression in the leaf of Lolium temulentum L. Ceres during the photoperiodic induction of flowering

Périlleux¹,

Ongena²,

Bernier³

1996

Planta

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Abstract. Unifoliated plants of Lolium temulentum L.Ceres were induced to flower by a unique 24-h long day (LD) consisting of the extension of the regular 8-h short day (SD) (400 I.tmol photons-m-2-s 1, fluorescence + incandescence) with incandescence at 10-15 gmolphotons. m-2.s-~. The polyadenylated-RNA complement of leaf blade tissues was analysed at 4-h intervals during the photoperiod extension in LD vs. SD, by using two-dimensional polyacrylamide gel electrophoresis to resolve in-vitro-translated products. Of the 991 spots that were analysed, none appeared or disappeared during the inductive cycle, i.e. no qualitative effect of floral induction was detected, at any time. Sixty-eight spots were found whose intensity was influenced by lengthening of the photoperiod; 50 of them, i.e. ca. 5% of the population analysed, were affected before the end of the extension period and were thus potentially related to floral induction. Many of these RNAs were not quantitatively constant during a 24-h cycle in SD. Seven of them oscillated according to the 'light-on' and the 'light-off" signals, among which three seemed to be controlled by phytochrome since their relative amount increased under the standard light conditions but decreased under incandescence even faster than in darkness. The large majority of other RNAs varied with a timing that was not clearly driven by the alternation of light and darkness, indicating that genes related to the biological clock may be especially sensitive to the lengthening of the photoperiod. Furthermore, seven spots were observed that underwent a phase-shift in LD, which consisted, for six of them, of a phase advance of 4-8 h. The steady-state level of CAB mRNA was analysed because the CAB gene family (encoding the chlorophyll a/b-binding proteins of the light-harvesting complexes) is known to be controlled both by the biological clock and phytochrome. In SD, the level was high in the light and low in Abbreviations: CAB = chlorophyll a/b-binding proteins of the light-harvesting complexes; 2D = two-dimensional; LD(s)= long day(s); LDP(s) = long day plant(s); SD(s) = short day(s); SDP(s) = short day plant(s) Correspondence to: C. P6rilleux; FAX: 32 (41) 66 38 31; E-mail: cperille@vm 1.ulg.ac.be darkness; the fluctuation was conducted by a circadian rhythm. When plants were exposed to the inductive LD, the peak of mRNA accumulation that was expected according to the endogenous rhythmicity was abolished, possibly because of the change in light quality during the LD extension.

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“…35 S]Met in vivo (Ono et al, 1993). The level of the corresponding mRNA increased during the flower-inductive dark period and also showed circadian oscillations (Ono et al, 1996).…”

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confidence: 97%

“…We also predicted the existence of rare transcripts that are related to flowering (Ono et al, 1993). Therefore, to examine this issue we decided to exploit a method for the isolation of preferentially expressed mRNAs, namely, differential display by PCR (Liang and Pardee, 1992).…”

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confidence: 99%

Accumulation of a Clock-Regulated Transcript during Flower-Inductive Darkness in Pharbitis nil1

et al. 1998

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To clarify the molecular basis of the photoperiodic induction of flowering in the short-day plant Pharbitis nil cv Violet, we examined changes in the level of mRNA in cotyledons during the flowerinductive photoperiod using the technique of differential display by the polymerase chain reaction. A transcript that accumulated during the inductive dark period was identified and a cDNA corresponding to the transcript, designated PnC401 (P. nil C401), was isolated. RNA-blot hybridization verified that levels of PnC401 mRNA fluctuated with a circadian rhythm, with maxima between 12 and 16 h after the beginning of the dark period) and minima of approximately 0. This oscillation continued even during an extended dark period but was damped under continuous light. Accumulation of PnC401 mRNA was reduced by a brief exposure to red light at the 8th h of the dark period (night-break treatment) or by exposure to far-red light at the end of the light period (end-of-day far-red treatment). These results suggest that fluctuations in levels of PnC401 mRNA are regulated by phytochrome(s) and a circadian clock and that they are associated with photoperiodic events that include induction of flowering.

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Changes in polypeptides in Pharbitis cotyledons during the first flower-inductive photoperiod

Cited by 12 publications

References 17 publications

Isolation of a CONSTANS Ortholog from Pharbitis nil and Its Role in Flowering

Isolation of a CONSTANS Ortholog from Pharbitis nil and Its Role in Flowering

Changes in gene expression in the leaf of Lolium temulentum L. Ceres during the photoperiodic induction of flowering

Accumulation of a Clock-Regulated Transcript during Flower-Inductive Darkness in Pharbitis nil1

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