Changes in Platelet Life Span in Dogs with Mitral Valve Regurgitation

Tanaka, Ryou; Murota, Ayako; Nagashima, Yukiko; Yamane, Yoshihisa

doi:10.1892/0891-6640(2002)016<0446:ciplsi>2.3.co;2

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…23 –26 In dogs with mitral valve disease, intramural infarction occurs not uncommonly, potentially as a result of reduced lumen diameter of remodeled coronary arteries causing reduced oxygen delivery, endothelial dysfunction, and increased vascular stiffness. 18,27 –29 In the young beagle dogs described in this report, medial hypertrophy occurs not as a result of an identified pathologic condition, but likely as a physiologic response to pressure within the PM.…”

Section: Discussionmentioning

confidence: 86%

Characterization of Spontaneous Vascular Findings in the Papillary Muscles of Beagle Dogs

Kohnken

Weber

2020

Toxicol Pathol

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Standard histology trimming practices for toxicity studies in dogs include preparation of a section of the papillary muscle associated with the left ventricular free wall—the anteriolateral or anterior muscle. In contrast, the posteromedial or posterior papillary muscle, associated with the interventricular septum, is not commonly evaluated. In humans, the posterior papillary muscle is more often affected in ischemic myocardial disease, in large part due to the absence of collateral circulation, in contrast to the anterior muscle. Due to the differential vascular supply to the papillary muscles, we sought to determine whether there is a higher incidence of spontaneous coronary vascular changes in the posterior papillary muscle versus the anterior muscle in dogs. The hearts of 30 vehicle-treated or untreated beagle dogs that were euthanized for other purposes were collected and sectioned in a consistent manner to include both papillary muscles for histologic evaluation. The posterior muscle consistently had higher incidences of intramural coronary arteries affected by vascular medial hypertrophy, medial arteriosclerosis, intimal hyperplasia, and/or disruption or loss of the internal elastic lamina. This observation may have significant implications for the evaluation and characterization of spontaneous and xenobiotic-induced cardiovascular lesions in dogs.

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Section: Discussionmentioning

confidence: 86%

Characterization of Spontaneous Vascular Findings in the Papillary Muscles of Beagle Dogs

Kohnken

Weber

2020

Toxicol Pathol

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“…During the progression of CHF, turbulent high velocity blood ow and changes in blood shear stress around the degenerative mitral valve lea ets may produce platelet activation in dogs (4,5). Although clinical relevance of the platelet activation is not clear (6), some studies in this species have shown that activated platelets might contribute to the disease progression and an increased risk of sudden death by development of intra-myocardial coronary arteriosclerosis and micro-thrombi due to CHF (4-8).…”

Section: Introductionmentioning

confidence: 99%

Platelet proteome changes in dogs with congestive heart failure

Levent

Kocatürk

Akgun

et al. 2020

Preprint

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Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in congestive heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n=10) and dogs with acute CHF due to myxomatous mitral valve disease (MMVD, n=10) were used. Acute CHF was defined based on the clinical (increased respiratory rate or difficulty breathing) and radiographic findings of pulmonary edema. Dogs Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris. Results: Totally 104 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in acute CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in acute CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses.Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with acute CHF due to MMVD. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF caused by MMVD and could be a tool for further investigations about the prevention and treatment of this disease.

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“…2 Both conditions cause turbulent high-velocity flow and high fluid shear stress, which in turn might cause platelet activation. 3 Indeed, dogs with MVP have been shown to have platelets with an increased aggregation response 4 and decreased life span, 5 indicating increased platelet activity. Conversely, dogs with symptomatic mitral regurgitation (MR) and Cavalier King Charles Spaniels (Cavaliers) with and without heart murmurs had decreased platelet-aggregation responses.…”

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confidence: 99%

Dogs with Heart Diseases Causing Turbulent High-Velocity Blood Flow Have Changes in Platelet Function and von Willebrand Factor Multimer Distribution

Tarnow¹,

Kristensen²,

Olsen³

et al. 2005

J Vet Int Med

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The purpose of this prospective study was to investigate platelet function using in vitro tests based on both high and low shear rates and von Willebrand factor (vWf) multimeric composition in dogs with cardiac disease and turbulent high-velocity blood flow. Client-owned asymptomatic, untreated dogs were divided into 4 groups: 14 Cavalier King Charles Spaniels (Cavaliers) with mitral valve prolapse (MVP) and no or minimal mitral regurgitation (MR), 17 Cavaliers with MVP and moderate to severe MR, 14 control dogs, and 10 dogs with subaortic stenosis (SAS). Clinical examinations and echocardiography were performed in all dogs. PFA100 closure times (the ability of platelets to occlude a hole in a membrane at high shear rates), platelet activation markers (plasma thromboxane B2 concentration, platelet surface P-selectin expression), platelet aggregation (in whole blood and platelet-rich plasma with 3 different agonists), and vWf multimers were analyzed. Cavaliers with moderate to severe MR and dogs with SAS had longer closure times and a lower percentage of the largest vWf multimers than did controls. Maximal aggregation responses were unchanged in dogs with SAS but enhanced in Cavaliers with MVP (regardless of MR status) compared with control dogs. No significant difference in platelet activation markers was found among groups. The data suggest that a form of platelet dysfunction detected at high shear rates was present in dogs with MR and SAS, possibly associated with a qualitative vWf defect. Aggregation results suggest increased platelet reactivity in Cavaliers, but the platelets did not appear to circulate in a preactivated state in either disease.

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Changes in Platelet Life Span in Dogs with Mitral Valve Regurgitation

Cited by 7 publications

References 33 publications

Characterization of Spontaneous Vascular Findings in the Papillary Muscles of Beagle Dogs

Characterization of Spontaneous Vascular Findings in the Papillary Muscles of Beagle Dogs

Platelet proteome changes in dogs with congestive heart failure

Dogs with Heart Diseases Causing Turbulent High-Velocity Blood Flow Have Changes in Platelet Function and von Willebrand Factor Multimer Distribution

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