Changes in Plasma Membrane Fluidity of Immortal Rodent Cells Induced by Anticancer Drugs Doxorubicin, Aclarubicin and Mitoxantrone

Jedrzejczak, M; Koceva‐Chyła, Aneta; Gwoździński, Krzysztof; Jóźwiak, Zofia

doi:10.1006/cbir.1999.0399

Cited by 19 publications

(13 citation statements)

References 31 publications

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“…17 Therefore, one possible result of these changes in membrane fluidity is linked to alterations in the physiological processes of the cell membrane, such as carrier-mediated transport, activity of membranebound enzyme, and membrane fluorescence anisotropy. 16,17 This linkage was also revealed after cells FIGURE 4. After 48 h of SMF exposure: (a) the alkaline phosphatase activity of the SMF-exposed cells was significantly greater than that of the controls (n = 4; *p < 0.05); the greatest difference between the untreated control (b) and SMF-exposed cells (c) was in the extracellular areas from TEM.…”

Section: Discussionmentioning

confidence: 69%

Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Chiu

Lee

et al. 2007

Ann Biomed Eng

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The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field (SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the changes in alkaline phosphatase activity and electron microscopy images. Membrane fluidity was used to evaluate alterations in the biophysical properties of the cellular membranes after the SMF simulation. Our results show that SMF exposure increases alkaline phosphatase activity and extracellular matrix release in MG63 cells. On the other hand, MG63 cells exposed to a 0.4-T SMF exhibited a significant increase in fluorescence anisotropy at 6 h, with a significant reduction in the proliferation effects of growth factors noted at 24 h. Based on these findings, the authors suggest that one of the possible mechanisms that SMF affects osteoblastic maturation is by increasing the membrane rigidity and reducing the proliferation-promoting effects of growth factors at the membrane domain.

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Section: Discussionmentioning

confidence: 69%

Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Chiu

Lee

et al. 2007

Ann Biomed Eng

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“…Short 2 h incubations of cells with low doxorubicin concentrations (0.5 and 1 μmol/L) caused an increase in membrane fluidity, while higher drug concentrations (above 5 μmol/L) induced a decrease in membrane fluidity. The most notable changes were observed for 10 and 20 μmol/L DOX concentrations (Jędrzejczak et al 1999). Cells treated with 7, 50 and 100 μmol/L H 2 O 2 were used for comparison.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Different effectiveness of piperidine nitroxides against oxidative stress induced by doxorubicin and hydrogen peroxide

et al. 2007

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The piperidine nitroxides Tempamine and Tempace have been studied for their effect on doxorubicin (DOX) and hydrogen peroxide (H(2)O(2)) cytotoxicity in immortalized B14 cells, a model for neoplastic phenotype. The significance for nitroxide performance of the substituent in position 4 of the piperidine ring was evaluated. The cells were exposed to DOX/H(2)O(2) alone or in combination with the nitroxides Tempamine or Tempace. Two other piperidine nitroxides, Tempo and Tempol, were used for comparison. All the nitroxides except Tempamine modestly reduced DOX cytotoxicity. Tempamine evoked a biphasic response: at concentrations lower than 200 micromol/L the nitroxide decreased DOX cytotoxicity, while at concentrations higher than 200 micromol/L, it enhanced DOX cytotoxicity. In contrast to Tempo and Tempol, Tempamine and Tempace ameliorated hydrogen peroxide cytotoxicity, but none of the nitroxides influenced TBARS stimulated by hydrogen peroxide. The cytoprotective effect of Tempace, Tempo and Tempol in DOX-treated cells correlated with the inhibition of DOX-induced lipid peroxidation. The bioreduction rates of the investigated nitroxides differed significantly and were variously affected by DOX depending on the nitroxide substituent. In combination with DOX, Tempo and Tempol were reduced significantly more slowly, while no influence of DOX on Tempamine and Tempace bioreduction was observed. Our results suggest that the structure of the 4-position substituent is an important factor for biological activity of piperidine nitroxides. Among the investigated nitroxides, Tempace displayed the best protective properties in vitro but Tempamine was the only nitroxide that potentiated cytotoxicity of DOX and did not influence DOX-induced lipid peroxidation. However, this nitroxide showed different performance depending on its concentration and conditions of oxidative stress.

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“…Hydrophobic interactions, membrane fluidity, and drug lipophilicity are decisive for efficient intracellular uptake of anticancer drugs (Jedrzejczak et al 1999;Schuldes et al 2001). Additionally, membrane fluidity studies have also been considered as a promising approach for cancer therapy (Ferreira et al 2005).…”

mentioning

confidence: 99%

Concentration Dependent Different Action of Tamoxifen on Membrane Fluidity

Kazancı

Severcan

2007

Bioscience Reports

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Tamoxifen (TAM) is a non-steroidal antiestrogen drug, which is widely used to prevent and treat breast, liver, pancreas and brain cancers. The present work investigates, in detail, the concentration dependent behavior of TAM (varying from 1 mol% to 45 mol%) on membrane fluidity. The differential scanning calorimetry (DSC) studies showed that tamoxifen eliminates the pre-transition and decreases the main phase transition to lower temperatures. Using visible spectroscopy at 440 nm and Fourier transform infrared (FTIR) spectroscopy it was found that membrane dynamics decreases for 1 and 3 mol% tamoxifen in both the gel and liquid crystalline phases. Above these concentrations up to 18-24 mol%, it increases and reaches its maximum values. As tamoxifen concentration was further increased, the membrane dynamics is found to be gradually decreased, although TAM still has fluidifying effect in comparison to pure phospholipid membrane. These findings are important for the effective use of tamoxifen in the cancer therapy to eliminate its dose dependent side effects reported in the literature.

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Changes in Plasma Membrane Fluidity of Immortal Rodent Cells Induced by Anticancer Drugs Doxorubicin, Aclarubicin and Mitoxantrone

Cited by 19 publications

References 31 publications

Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Different effectiveness of piperidine nitroxides against oxidative stress induced by doxorubicin and hydrogen peroxide

Concentration Dependent Different Action of Tamoxifen on Membrane Fluidity

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