Changes in plasma levels of hepatocyte growth factor and its associated factors during pregnancy

Watanabe, Takashi; Sakata, Yoichi; Matsubara, Shigeki; Yamagishi, Toshiya; Nagaike, Kazuhiro; Kuwata, Tomoyuki; Suzuki, Mitsuaki

doi:10.1111/j.1447-0756.2006.00346.x

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…5). These included cadherin-5, which is found at 0.3-4.9 ng/mL, neutrophil gelatinase-associated lipocalin with levels estimated at ∼2.5 ng/mL, and hepatocyte growth factor activator, which is present at 3-6 ng/mL [45-47]. …”

Section: Resultsmentioning

confidence: 99%

A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma

Drake

Schilling

Niles

et al. 2011

Analytical Biochemistry

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Glycans are cell-type specific, post-translational protein modifications that are modulated during developmental and disease processes. As such, glycoproteins are attractive biomarker candidates. Here, we describe a mass spectrometry-based workflow that incorporates lectin affinity chromatography to enrich for proteins that carry specific glycan structures. As increases in sialylation and fucosylation are prominent among cancer-associated modifications, we focused on Sambucus nigra agglutinin (SNA) and Aleuria aurantia lectin (AAL), lectins which bind sialic acid- and fucose-containing structures, respectively. Fucosylated and sialylated glycopeptides from human lactoferrin served as positive controls, and high mannose structures from yeast invertase served as negative controls. The standards were spiked into Multiple Affinity Removal System (MARS) 14-depleted, trypsin-digested human plasma from healthy donors. Samples were loaded onto lectin columns, separated by HPLC into flow-through and bound fractions, and treated with peptide: N-glycosidase F to remove N-linked glycans. The deglycosylated peptide fractions were interrogated by ESI HPLC-MS/MS. We identified a total of 122 human plasma glycoproteins containing 247 unique glycosites. Importantly, several of the observed glycoproteins (e.g., cadherin 5 and neutrophil gelatinase-associated lipocalin) typically circulate in plasma at low ng/mL levels. Together, these results provide mass spectrometry-based evidence of the utility of incorporating lectin-separation platforms into cancer biomarker discovery pipelines.

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Section: Resultsmentioning

confidence: 99%

A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma

Drake

Schilling

Niles

et al. 2011

Analytical Biochemistry

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“…In the literature, it can be found few and contradictory reports about plasma HGF levels during normal pregnancy and in pregnancies complicated with obesity. In normal pregnancy, HGF levels increased with gestational age until term [18,19] or there was no change during pregnancy [20]. In pregnancies complicated with obesity, HGF levels were similar to normal women and remained unchanged with increasing BMI during the second trimester of pregnancy [21].…”

mentioning

confidence: 92%

Hepatocyte growth factor is elevated in amniotic fluid from obese women and regulates placental glucose and fatty acid metabolism

et al. 2015

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Mitochondrial Function and Resistance to Oxidative Stress in the Kidney during Pregnancy

Popkov,

Buyan,

Makievskaya

et al. 2024

Bull Exp Biol Med

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Changes in plasma levels of hepatocyte growth factor and its associated factors during pregnancy

Abstract: A high concentration of HAI-1 during pregnancy may decrease the plasma level of active HGFA. This may in turn inhibit the activation of the precursor form of HGF, leading to a decrease in the level of plasma active HGF.

Cited by 8 publications

References 23 publications

A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma

A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma

Hepatocyte growth factor is elevated in amniotic fluid from obese women and regulates placental glucose and fatty acid metabolism

Mitochondrial Function and Resistance to Oxidative Stress in the Kidney during Pregnancy

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